Molecular identification of airborne bacteria associated with aerial spraying of bovine slurry waste employing 16S rRNA gene PCR and gene sequencing techniques

Murayama, Mayumi; Kakinuma, Yuki; Maeda, Yasunori; Rao, J. R.; Matsuda, Motoo; Xu, Jiru; Moore, Peter J.A.; Millar, B. Cherie; Rooney, Paul J.; Goldsmith, Colin E.; Loughrey, Anne; McMahon, M.A.S.; McDowell, D.A.; Moore, John E.

doi:10.1016/j.ecoenv.2009.10.003

Cited by 21 publications

(11 citation statements)

References 18 publications

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“…This result is similar to recent PCR-based aerosol studies conducted at dairies and during the aerial spreading of dairy slurry (Murayama et al, 2010;Ravva et al, 2011). In both cases, the authors did not detect either foodborne pathogens or pathogens associated with an inhalation route of transmission.…”

Section: Resultssupporting

confidence: 78%

“…To date, relatively few PCR-based studies have been conducted to identify airborne bacteria near animal operations and wastewater spray irrigation sites (Murayama et al, 2010;Ravva et al, 2011). Compared to culture-dependent approaches, molecular techniques allow investigators to tentatively identify a wider range of organisms that could not normally be isolated using traditional growth mediums (Dungan and Leytem, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Use of a culture-independent approach to characterize aerosolized bacteria near an open-freestall dairy operation

Dungan

2012

Environment International

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Animal manures are known to harbor a variety of zoonotic pathogens, which are suspected of being transported off-site as aerosols from confined feeding operations. In this study, aerosols were collected using a high-volume sampler downwind from a 10,000 cow open-freestall dairy and nearby fields being sprinkler irrigated with wastewater. DNA extracts were prepared from the aerosol samples, then a region of the16S ribosomal RNA gene was sequenced for bacterial identification and phylogenetic classification. At the dairy and irrigation sites, Proteobacteria (α-, β-, and γ-subdivisions) was the most abundant phylum, representing 78% and 69% of all sequences, respectively, while Actinobacteria, Bacteroidetes and Firmicutes represented only 10% or less of the sequences. Of the 191 clones sequenced from the dairy aerosol samples, 6 sequences were found to be homologous with uncultured bacteria from cow milk, rumen, and fecal samples. However, none of the sequence matches was affiliated with bacteria known to be pathogenic to otherwise healthy humans. Although our results do suggest a high diversity among the aerosolized bacteria, the sampling strategy employed in this study may not account for the variable nature of bioaerosol emissions.

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Section: Resultssupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Use of a culture-independent approach to characterize aerosolized bacteria near an open-freestall dairy operation

Dungan

2012

Environment International

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“…Using polymerase chain reaction (PCR) to amplify 16$ ribosomal RNA genes in air samples collected immediately adjacent to the spreading of swine and dairy cattle slurry. pathogens having an aerogenic route of infection were not identified (Murayama et al .. 2010;Dungan, 2012). While results from these and other studies suggest a low risk for exposure to pathogens (Brooks et al .. 2005: Tanner et al, 2005, significant knowledge gaps s till exist with respect to the fate and transport of bioaerosols, making it difficult to predicate the health risks associated with aerosolized pathogens accurately (Pillai and Ricke.…”

Section: Aerosolization Of Pathogensmentioning

confidence: 86%

Sustainable manure management.

Leytem¹,

Dungan²,

Kleinman³

2013

Sustainable Animal Agriculture

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“…based on conventional methods is difficult and should be confirmed with molecular assays. Bacteria in this genus can be misidentified as Oerskovia xanthineolytica when the API Coryne kit (bioMérieux, Marcy l’Étoile, France) is used ( 7 ); 16S rRNA gene sequencing seems to be useful for identification of E. sibiricum ( 8 ). Consequently, the frequency of this infection can be underdiagnosed.…”

mentioning

confidence: 99%