Molecular identification of Anisakis type I larvae isolated from hairtail fish off the coasts of Taiwan and Japan

Umehara, Azusa; Kawakami, Yasushi; Ooi, Hong-Kean; Uchida, Akihiko; Ohmae, Hiroshi; Sugiyama, Hiromu

doi:10.1016/j.ijfoodmicro.2010.08.011

Cited by 49 publications

(40 citation statements)

References 25 publications

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“…The high prevalence of Anisakis species in fish hosts in this study is therefore thought to reflect the richness of micro/macroinvertebrates and possibly subsequently have relations with the biomass of other predators such as pinnipeds and cetaceans in waters investigated. Several papers also reported relatively a high prevalence of anisakid nematodes larvae in the largehead hairtail (Borges et al 2012;Kong et al 2015;Umehara et al 2010), as in this study. The low number of the nematodes larvae in the previous study (Lee et al 2009) may just reflect the low abundance of micro/macroinvertebrates in the areas where the largehead hairtail were caught.…”

Section: Discussionsupporting

confidence: 83%

“…Several papers described anisakid nematodes L3 from the largehead hairtail (Borges et al 2012;Kong et al 2015;Lee et al 2009;Shih 2004;Umehara et al 2010;Zhang et al, 2013), but there was no report in which T. japonicus was mentioned as host species, except for our study. It is not clear if the host species mentioned was in fact T. japonicus or other Trichiurus species in their studies because the identification of host species was not clearly conducted.…”

Section: Discussionmentioning

confidence: 55%

“…There have been many papers regarding anisakid nematodes isolated from Trichiurus species (Borges et al 2012;Kong et al 2015;Lee et al 2009;Shih 2004;Umehara et al 2010;Zhang et al 2013). But, the identification of host species was not clearly conducted, and several scientific names (e.g., Trichiurus lepturus, Trichiurus haumela, Trichiurus sp.)…”

Section: Introductionmentioning

confidence: 99%

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Genetic identification of anisakid nematodes isolated from largehead hairtail (Trichiurus japonicus) in Korea

2016

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Background: The nematode species belonging to genus Anisakis occur at their third larval stage in numerous marine teleost fish species worldwide and known to cause accidental human infection through the ingestion of raw or undercooked fish or squids. They may also draw the attention of consumers because of the visual impact of both alive and dead worms. Therefore, the information on their geographical distribution and clear species identification is important for epidemiological survey and further prevention of human infection. Results: For identification of anisakid nematodes species isolated from largehead hairtail (Trichiurus japonicus), polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of internal transcribed spacers of ribosomal DNA were conducted. Mitochondrial cytochrome c oxidase subunit 2 gene was also sequenced, and phylogenetic analysis was conducted. From the largehead hairtail (n = 9), 1259 nematodes were isolated in total. Most of the nematodes were found encapsulated throughout the viscera (56.2 %, 708/1259) or moving freely in the body cavity (41.5 %, 523/1259), and only 0.3 % (4/1259) was found in the muscles. By PCR-RFLP, three different nematode species were identified. Anisakis pegreffii was the most dominantly found (98. 7 %, 1243/1259) from the largehead hairtail, occupying 98.7 % (699/708) of the nematodes in the mesenteries and 98.1 % (513/523) in the body cavity. Hybrid genotype (Anisakis simplex × A. pegreffii) occupied 0.5 %, and Hysterothylacium sp. occupied 0.2 % of the nematodes isolated in this study. Conclusions:The largehead hairtail may not significantly contribute accidental human infection of anisakid nematode third stage larvae because most of the nematodes were found from the viscera or body cavity, which are not consumed raw. But, a high prevalence of anisakid nematode larvae in the largehead hairtail is still in concern because they may raise food safety problems to consumers. Immediate evisceration or freezing of fish after catch will be necessary before consumption.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 55%

Section: Introductionmentioning

confidence: 99%

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Genetic identification of anisakid nematodes isolated from largehead hairtail (Trichiurus japonicus) in Korea

2016

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“…In PCR-RFLP analysis, primer pairs A-B and NC2-NC5 were used for the amplification of ITS region, AniF-Ani1R for 5.8 rRNA and JB3-JB4.5 for mt-CO1 genes 7,8,[14][15][16][22][23][24][25] . The most used restriction enzymes were HinfI, HhaI and TagI for the analysis of both ribosomal and mitochondrial genes 7,8,[14][15][16][22][23][24][25] 7,[14][15][16]22,25 .…”

Section: Umehara Et Almentioning

confidence: 99%

“…The most used restriction enzymes were HinfI, HhaI and TagI for the analysis of both ribosomal and mitochondrial genes 7,8,[14][15][16][22][23][24][25] 7,[14][15][16]22,25 . In this study, we used universal primers A and B for the amplification of ITS region, and HinfI for digestion of ITS products.…”

Section: Umehara Et Almentioning

confidence: 99%

Türkiye’nin Erzurum İlinde İnsan Tüketimi İçin Satılan İstavrit Balıklarında (Trachurus trachurus) Anisakis pegreffii’nin Moleküler Tanısı

Ütük¹,

Pişkin²,

Balkaya³

2009

Kafkas Univ Vet Fak Derg

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SummaryThis study was planned to identify six parasites detected in horse mackerels (Trachurus trachurus) sold for human consumption in Erzurum province. DNA extraction was performed on six parasites diagnosed as nematode. Species identification was done by PCR and PCR-RFLP analysis of ITS (ITS-1, 5.8S subunit rRNA gene and ITS-2) region of ribosomal DNA (rDNA) and partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) and mt-CO2 genes. According to the PCR and PCR-RFLP results, all parasites were identified as Anisakis pegreffii. Partial sequence of mt-CO1 gene of one randomly selected parasite was corresponding with A. simplex and mt-CO2 genes of two parasites were corresponding with A. pegreffii. With this study, A. pegreffii was molecularly detected for the first time in Turkey. Keywords: Anisakis pegreffii, Fish, PCR, PCR-RFLP, Sequencing Türkiye'nin Erzurum İlinde İnsan Tüketimi İçin Satılan İstavrit Balıklarında (Trachurus trachurus) Anisakis pegreffii'nin Moleküler Tanısı ÖzetBu çalışma, Erzurum ilinde insan tüketimi için satılan istavrit balıklarında (Trachurus trachurus) tespit edilen altı parazitin tür tanısını yapmak amacıyla planlanmıştır. Nematod olarak belirlenen altı parazitten DNA ekstraksiyonu yapılmıştır. Tür tanısı, rDNA'nın ITS bölgelerinin (ITS1, 5.8 alt ünite rRNA ve ITS2) PCR ve PCR-RFLP analizleri ve mitokondrial sitokrom c oksidaz alt ünite 1 (mt-CO1) ve mt-CO2 genlerinin kısmi olarak sekanslanması ile yapılmıştır. PCR ve PCR-RFLP sonuçlarına göre tüm parazitlerin Anisakis pegreffii olduğu belirlenmiştir. Rastgele seçilen bir parazitin mt-CO1 geninin kısmi sekans sonucu A. simplex, iki parazitin mt-CO2 geninin kısmi sekans sonuçları ise A. pegreffii ile uyuşmuştur. Bu çalışma ile A. pegreffii Türkiye'de ilk kez moleküler olarak tespit edilmiştir.

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Occurrence of Hysterothylacium and Anisakis nematodes (Ascaridida: Ascaridoidea) in the tanaka’s snailfish Liparis tanakae (Gilbert & Burke) (Scorpaeniformes: Liparidae)

Guo

Zhang

et al. 2014

Parasitol Res

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The tanaka's snailfish Liparis tanakae (Gilbert & Burke) (Scorpaeniformes: Liparidae) is an economically important marine fish species in China. However, the helminth parasites of this fish are still poorly known. During a helminthological survey of Chinese marine fishes from 2011 to 2012, we revealed that L. tanakae was heavily infected with third-stage larvae and adults of ascaridoid nematodes (total prevalence 100% and mean intensity 82.3 nematodes per fish). Four species of third-stage larvae Hysterothylacium liparis Li, Xu & Zhang, 2007, H. aduncum (Rudolphi, 1802), Hysterothylacium fabri (Rudolphi, 1819), and Anisakis pegreffii (Campana-Rouget & Biocca, 1955) and a single species of adults H. liparis were differentiated and identified by morphological and molecular methods. The detailed morphology of the four species of third-stage larvae was also studied using light microscopy and scanning electron microscopy. The morphological and molecular characterization of the third-stage larvae of H. liparis was reported. Liparis tanakae represents a new host record for A. pegreffii and H. fabri. In addition, a new name, Hysterothylacium zhoushanense nom. nov. was also given to Hysterothylacium zhoushanensis Li, Liu & Zhang, 2012 to make the latinized specific epithet agree with this neuter generic name.

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Molecular identification of Anisakis type I larvae isolated from hairtail fish off the coasts of Taiwan and Japan

Cited by 49 publications

References 25 publications

Genetic identification of anisakid nematodes isolated from largehead hairtail (Trichiurus japonicus) in Korea

Genetic identification of anisakid nematodes isolated from largehead hairtail (Trichiurus japonicus) in Korea

Türkiye’nin Erzurum İlinde İnsan Tüketimi İçin Satılan İstavrit Balıklarında (Trachurus trachurus) Anisakis pegreffii’nin Moleküler Tanısı

Occurrence of Hysterothylacium and Anisakis nematodes (Ascaridida: Ascaridoidea) in the tanaka’s snailfish Liparis tanakae (Gilbert & Burke) (Scorpaeniformes: Liparidae)

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