Molecular identification of burn spot disease syndrome agent Fusarium avenaceum in Turkish crayfish Pontastacus leptodactylus populations

Taştan, Büşra; Akhan, Süleyman

doi:10.3354/dao03570

Cited by 2 publications

(1 citation statement)

References 16 publications

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“…Due to overall lack of research of crayfish mycobiome, and the absence of cultivation-independent data based on high throughput sequencing, only limited comparative information was available in the literature, except for several studies that analyzed fungal isolates from crayfish by sequencing the ITS region (e.g. [ 52 , 53 ]).…”

Section: Discussionmentioning

confidence: 99%

The mycobiome of a successful crayfish invader and its changes along the environmental gradient

et al. 2023

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Background The microbiome plays an important role in biological invasions, since it affects various interactions between host and environment. However, most studies focus on the bacteriome, insufficiently addressing other components of the microbiome such as the mycobiome. Microbial fungi are among the most damaging pathogens in freshwater crayfish populations, colonizing and infecting both native and invasive crayfish species. Invading crayfish may transmit novel fungal species to native populations, but also, dispersal process and characteristics of the novel environment may affect the invaders’ mycobiome composition, directly and indirectly affecting their fitness and invasion success. This study analyzes the mycobiome of a successful invader in Europe, the signal crayfish, using the ITS rRNA amplicon sequencing approach. We explored the mycobiomes of crayfish samples (exoskeletal biofilm, hemolymph, hepatopancreas, intestine), compared them to environmental samples (water, sediment), and examined the differences in fungal diversity and abundance between upstream and downstream segments of the signal crayfish invasion range in the Korana River, Croatia. Results A low number of ASVs (indicating low abundance and/or diversity of fungal taxa) was obtained in hemolymph and hepatopancreas samples. Thus, only exoskeleton, intestine, sediment and water samples were analyzed further. Significant differences were recorded between their mycobiomes, confirming their uniqueness. Generally, environmental mycobiomes showed higher diversity than crayfish-associated mycobiomes. The intestinal mycobiome showed significantly lower richness compared to other mycobiomes. Significant differences in the diversity of sediment and exoskeletal mycobiomes were recorded between different river segments (but not for water and intestinal mycobiomes). Together with the high observed portion of shared ASVs between sediment and exoskeleton, this indicates that the environment (i.e. sediment mycobiome) at least partly shapes the exoskeletal mycobiome of crayfish. Conclusion This study presents the first data on crayfish-associated fungal communities across different tissues, which is valuable given the lack of studies on the crayfish mycobiome. We demonstrate significant differences in the crayfish exoskeletal mycobiome along the invasion range, suggesting that different local environmental conditions may shape the exoskeletal mycobiome during range expansion, while the mycobiome of the internal organ (intestine) remained more stable. Our results provide a basis for assessing how the mycobiome contributes to the overall health of the signal crayfish and its further invasion success.

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Section: Discussionmentioning

confidence: 99%

The mycobiome of a successful crayfish invader and its changes along the environmental gradient

et al. 2023

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Fusarium oxysporum causes black gill disease in narrow-clawed crayfish Pontastacus leptodactylus

Taştan

Çağatay

2022

Dis. Aquat. Org.

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Morphological and molecular analyses were carried out to determine the causative agent of black gill disease in narrow-clawed crayfish Pontastacus leptodactylus specimens collected from 6 lakes (Taşkısığı, Beyşehir, Karaidemir, Karataş, Manyas, and Gölhisar) in Turkey. Fungi were isolated from the tissues of crayfish displaying putative black gill disease symptoms. Morphological evaluation was conducted, and the isolates were determined to be similar to Fusarium oxysporum. Subsequent molecular cloning of the ITS region of nrDNA by PCR confirmed species identification; DNA sequences from all 6 isolates were 99% similar to those of F. oxysporum. An experimental infection trial was conducted in triplicate using 1 of the 6 isolates to fulfill Koch’s postulates. Three groups of crayfish were used: (1) wounded and contaminated (WC), (2) wounded and not contaminated (WNC), and (3) non-wounded and contaminated (NWC). On Day 3, one individual died in the WC group. Throughout the total 74 d monitoring period, no other mortality was recorded. On Day 14, all crayfish in the WC group showed blackened gills, whereas all crayfish in the NWC displayed partial blackening and all crayfish in the WNC group displayed normal gill coloration. F. oxysporum was re-isolated in pure culture from the WC and NWC groups; thus, the disease was confirmed. Our results indicated that F. oxysporum causes black gill disease in P. leptodactylus crayfish. Moreover, we demonstrated that F. oxysporum can also infect non-wounded crayfish.

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Molecular identification of burn spot disease syndrome agent Fusarium avenaceum in Turkish crayfish Pontastacus leptodactylus populations

Cited by 2 publications

References 16 publications

The mycobiome of a successful crayfish invader and its changes along the environmental gradient

The mycobiome of a successful crayfish invader and its changes along the environmental gradient

Fusarium oxysporum causes black gill disease in narrow-clawed crayfish Pontastacus leptodactylus

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