Molecular Identification of "Chuanxiong" by Nucleotide Sequence and Multiplex Single Base Extension Analysis on Chloroplast trnK Gene

Zhu, Shu; Fushimi, Hirotoshi; Han, Guiru; Tsuchida, Takashi; Uno, Toshihiko; Takano, Akihito; Komatsu, Kenshi

doi:10.1248/bpb.30.527

Cited by 7 publications

(7 citation statements)

References 17 publications

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“…These data are consistent with the results of FISH/GISH, indicating that molecular and cytogenetic relationships between these two species were much closer than between either of these species and A. polymorpha. A recent study proposed that C. officinale and L. chuanxiong had different origins based on an analysis of the chloroplast trnK gene (Zhu et al, 2007). Conversely, phylogenetic analyses of the species based on the sequences of the rDNA and other chloroplast DNA (rbcL and rpoC1) have indicated that the two species are grouped together, but also distantly related to A. polymorpha (Kondo et al, 1996;Downie et al, 1998;Xue et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Inter-genomic relationships among three medicinal herbs: Cnidium officinale, Ligusticum chuanxiong and Angelica polymorpha

Lee

Choi

Sung³

et al. 2010

Genes Genom

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Cnidium officinale, Ligusticum chuanxiong and Angelica polymorpha have long been used as Chinese medicinal herbs to treat the same conditions. To elucidate the inter-specific relationships among these species, molecular and molecular cytogenetic analyses were conducted. Cytogenetically, C. officinale and A. polymorpha were found to be diploid with 2n=2x=22, but L. chuanxiong had a triploid chromosome number (2n= 3x=33). FISH analysis revealed that each two pairs of the 45S-and 5S rRNA genes were detected on the different chromosomes of C. officinale, and that triplet of the rRNA genes were localized on the same loci of the homologue in L. chuanxiong. GISH conducted using a genomic DNA probe detected strong cross-hybridization of genomes between C. officinale and L. chuanxiong, while no distinct GISH signal between C. officinale and A. polymorpha was observed. ITS and NTS data also revealed a very high sequence homology (95−96%) between C. officinale and L. chuanxiong. The RFLP profile of the rDNA-ITS enabled us to confirm the genetic distance among species. An effective species-specific SCAR marker for the identification of the wild plant, A. polymorpha, was also developed. Taken together, the results of this study suggest two possibilities; (1) L. chuanxiong originated from C. officinale, or (2) L. Chuanxiong and C. officinale diverged from the same parental plant.

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Section: Discussionmentioning

confidence: 99%

Inter-genomic relationships among three medicinal herbs: Cnidium officinale, Ligusticum chuanxiong and Angelica polymorpha

Lee

Choi

Sung³

et al. 2010

Genes Genom

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“…Similarly, the subtle distinction of L. jeholens e and L. tenuissimum (Korean name, Go-Bon) 33 resulted in improper utilization and counterfeit medicines being sold frequently in China and Korea. So far, a few complicated methods have been proposed, including multiplex PCR 24 , 33 and high-performance liquid chromatography 34 for precise identification, which depends on stringently experimental conditions. Generally, the reliable phylogeny implications and accurate and effective plant identification for those species, in this context, has become progressively imperative.…”

Section: Introductionmentioning

confidence: 99%

Uncovering dynamic evolution in the plastid genome of seven Ligusticum species provides insights into species discrimination and phylogenetic implications

Yuan

Sha

Xiong

et al. 2021

Sci Rep

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Ligusticum L., one of the largest members in Apiaceae, encompasses medicinally important plants, the taxonomic statuses of which have been proved to be difficult to resolve. In the current study, the complete chloroplast genomes of seven crucial plants of the best-known herbs in Ligusticum were presented. The seven genomes ranged from 148,275 to 148,564 bp in length with a highly conserved gene content, gene order and genomic arrangement. A shared dramatic decrease in genome size resulted from a lineage-specific inverted repeat (IR) contraction, which could potentially be a promising diagnostic character for taxonomic investigation of Ligusticum, was discovered, without affecting the synonymous rate. Although a higher variability was uncovered in hotspot divergence regions that were unevenly distributed across the chloroplast genome, a concatenated strategy for rapid species identification was proposed because separate fragments inadequately provided variation for fine resolution. Phylogenetic inference using plastid genome-scale data produced a concordant topology receiving a robust support value, which revealed that L. chuanxiong had a closer relationship with L. jeholense than L. sinense, and L. sinense cv. Fuxiong had a closer relationship to L. sinense than L. chuanxiong, for the first time. Our results not only furnish concrete evidence for clarifying Ligusticum taxonomy but also provide a solid foundation for further pharmaphylogenetic investigation.

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“…[1314] Molecular results suggested that the origin of L. chuanxiong is C. officinale . [15] According to Lee et al .,[16] C. officinale and L. chuanxiong are closely related species with 98% of sequence identity. Previous studies from our laboratory showed that both C. officinale and L. chuanxiong had the same inhibitory activities on lipid peroxidation and there were no significant differences between two herbs.…”

Section: Introductionmentioning

confidence: 99%

Free radical scavenging activities of Cnidium officinale Makino and Ligusticum chuanxiong Hort. methanolic extracts

Mahesh¹,

Park²

2010

Phcog Mag

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Background:Antioxidants from natural resources possess multifaceted and importance of the activities provides substantial scope in neurodegenerative diseases. The aim of this study was to assess and compare the free radical scavenging activities of Cnidium officinale and Ligusticum chuanxiong, which are closely related species.Materials and Methods:The scavenging activities of plant materials were evaluated using Trolox equivalent antioxidant capacity (TEAC), oxygen radical absorbance capacity (ORAC) and 1,1-diphenyl-2-picrylhydrazyl (DPPH), superoxide radical (O2·-), hydrogen peroxide (H2O2), hydroxyl (OH·), nitric oxide radical (NO·) and metal chelation. In addition, the cell viability and nitric oxide release were assayed using Neuro-2a (N2a) cells.Results:The methanolic extracts of C. officinale and L. chuanxiong showed scavenging activities of free radicals with an additional antioxidant capacity. Moreover, the efficacy on the cell viability and nitric oxide release in cell culture model has been established.Conclusion:Results of the present study suggests that the extracts of C. officinale and L. chuanxiong have comparatively similar free radical scavenging activities in vitro and may have important health effects.

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Molecular Identification of "Chuanxiong" by Nucleotide Sequence and Multiplex Single Base Extension Analysis on Chloroplast trnK Gene

Cited by 7 publications

References 17 publications

Inter-genomic relationships among three medicinal herbs: Cnidium officinale, Ligusticum chuanxiong and Angelica polymorpha

Inter-genomic relationships among three medicinal herbs: Cnidium officinale, Ligusticum chuanxiong and Angelica polymorpha

Uncovering dynamic evolution in the plastid genome of seven Ligusticum species provides insights into species discrimination and phylogenetic implications

Free radical scavenging activities of Cnidium officinale Makino and Ligusticum chuanxiong Hort. methanolic extracts

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