Molecular identification of clinical Candida isolates by simple and randomly amplified polymorphic DNA-PCR

Al-Tekreeti, Afnan R. A.; Al-Halbosiy, Mohammad M. F.; Dheeb, Batol Imran; Hashim, Abdulkareem Jasim; Al-Zuhairi, Anaam Fuad Hussain; Mohammad, Farooq I.

doi:10.1007/s13369-017-2762-1

Cited by 21 publications

(22 citation statements)

References 18 publications

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“…Out of these 15 papers describing a safety concern for one or several QPS yeast species, 10 referred to Candida kefyr (teleomorph = Kluyveromyces marxianus ) (Al‐Tekreeti et al., ; Aslani et al., ; Charsizadeh et al., ,b,c; Jahanshiri et al., ; Nejat et al., ; Ortiz et al., ; Sav et al., ; Scapaticci et al., )), five to Saccharomyces cerevisiae of which one was identified as Saccharomyces boulardii (Aslani et al., ; Dangarembizi et al., ; Scapaticci et al., ; Sekyere and Asante, ; Wasilewska and Wroblewska, )), five to Candida famata (teleomorph = Debaryomyces hansenii ) (Kumari et al. (); (Mohamed et al., ; Rajkowska and Kunicka‐Styczyńska, ; Taverna et al., ; Vieira et al., ) and one to Hanseniaspora uvarum (Siavoshi et al., ).…”

Section: Assessmentmentioning

confidence: 99%

“…From the remaining 15 references that describe a safety concern, 13 were related to human health (Al‐Tekreeti et al., ; Aslani et al., ; Charsizadeh et al., ,b,c; Jahanshiri et al., ; Nejat et al., ; Ortiz et al., ; Sav et al., ; Scapaticci et al., ; Siavoshi et al., ; Taverna et al., ; Wasilewska and Wroblewska, ); one to animal health (Dangarembizi et al., ) and four to antimicrobial resistance (Sav et al., ; Scapaticci et al., ; Sekyere and Asante, ; Taverna et al., ).…”

Section: Assessmentmentioning

confidence: 99%

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Update of the list of QPS‐recommended biological agents intentionally added to food or feed as notified to EFSA 9: suitability of taxonomic units notified to EFSA until September 2018

Koutsoumanis¹,

Allende²,

Álvarez‐Ordóñez³

et al. 2019

EFS2

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The qualified presumption of safety (QPS) procedure was developed to provide a harmonised generic pre‐evaluation to support safety risk assessments of biological agents performed by EFSA's Scientific Panels. The taxonomic identity, body of knowledge, safety concerns and antimicrobial resistance were assessed. Safety concerns identified for a taxonomic unit are, where possible and reasonable in number, reflected by ‘qualifications’ which should be assessed at the strain level by the EFSA's Scientific Panels. During the current assessment, no new information was found that would change the previously recommended QPS taxonomic units and their qualifications. Between April and September 2018, the QPS notification list was updated with 48 microorganisms from applications for market authorisation. Of these, 30 biological agents already had QPS status, 15 were excluded from the QPS exercise by the previous QPS mandate (five filamentous fungi) or from further evaluations within the current mandate (two notifications of Enterococcus faecium, one of Streptomyces spp. and seven of Escherichia coli). One taxonomic unit was (re)evaluated: Pseudomonas fluorescens had been previously evaluated in 2016, and was now re‐evaluated within this mandate. The revision of the literature supports the previously identified safety concerns (e.g. production of biocompounds with antimicrobial activity and virulence features), preventing the inclusion of P. fluorescens in the QPS list. Mycobacterium setense and Komagataeibacter sucrofermentans were evaluated for the first time. M. setense cannot be considered for the QPS assessment because there are significant safety concerns. K. sucrofermentans (Acetobacter xylinus subsp. sucrofermentans) can be proposed for the QPS list but only for production purposes. The QPS status of Corynebacterium glutamicum is confirmed with the qualification extended to other production purposes.

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Section: Assessmentmentioning

confidence: 99%

Section: Assessmentmentioning

confidence: 99%

Update of the list of QPS‐recommended biological agents intentionally added to food or feed as notified to EFSA 9: suitability of taxonomic units notified to EFSA until September 2018

Koutsoumanis¹,

Allende²,

Álvarez‐Ordóñez³

et al. 2019

EFS2

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“…The results of this test showed that most of the isolates under test were sensitive to the group of antifungal included in the test card (AST-YS07 Card) and some of them showed Types of diagnosed isolates and their percentages moderate sensitivity with a slight increase in the MIC values of some antifungal by non-albicans candida species (NACsp). as shown in Table (1)(2)(3)(4). The main component of the essential oil under study is (1,.…”

Section: -Vitek2 Compact Sensitivity Testmentioning

confidence: 99%

Synergistic effect of tea tree oil on fungi causing vaginal thrush in pregnant women

Dahham¹,

Omar²,

Dheeb³

2019

JoBRC

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Background: Vulvovaginal candidiasis(VVC), or Vaginal thrush, is a vaginitis caused by the overgrowth of some opportunistic yeasts of the genus Candida spp. Australian Tea Tree Oil (TTO) is one of the most important essential oils that contain many compounds that are effective against many bacteria and fungi, which may match the effect of common antibiotics. Objective: The aim of this study was to isolate and diagnose fungi that causing vaginal thrush, in pregnant women and to study the effect of (pregnancy and pregnancy sequence and age) on the rate of infection compared to non-pregnant, as well as to study the drug sensitivity of isolates towards a number of antifungal and compared that with the inhibitory effectiveness of tea tree oil. Material and Methods: 75 vaginal swabs from pregnant women and 50 from non-pregnant women were collected at ages ranging from 17-65 years. The isolates were diagnosed using several methods, including the Vitek2 Compact system. 17 isolates were selected to study the inhibitory effect of ten antifungal agents, six of them were automatically tested by the Vitek 2 compact system, which contains a sensitivity test kit (AST-YS07 Card). The essential oil (TTO) was analyzed by GC-MS to detect its content of active compounds. The inhibitory effect of TTO was studied according to "Broth dilution" method to determine minimum inhibitory concentration (MIC) for it, the inhibitory effect of four concentrations of TTO (100%, 50%, 25%, 12.5 %) was also tested according to "well diffusion" method. This investigation also included a study of the mechanism of action (TTO). RESULTS: The rate of infection among pregnant women was (41%) with the highest rate of infection during the third trimester of pregnancy, while the percentage of non-pregnant women was only 8%. Vaginal infection was also prevalent in the age group ranged between (17-29 years).The isolates showed resistance to (Ketoconazol, Terbinafine) while they were sensitive to (Nystatin, clotrimazol), as well as sensitive to all antifungal of (AST-YS07 Card). The analysis of the TTO using chromatogram showed that it contains 32 chemical compounds, most of them are monoterpene like (Terpinen-4-ol, 1, 8-Cineol). The MIC of TTO ranged between (4 - < 2 µl/ ml). The function of TTO is to destroy the structural structure of the cell membrane and change its permeability, thereby leakage of cellular components and cell death. Conclusion: Pregnancy increases the rate of vaginal candidiasis in women, especially during the third trimester. TTO is highly effective in inhibiting the growth of opportunistic candida yeasts.

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“…It is inexpensive and requires less amount of DNA [2,3]. Moreover, RAPD analysis is technically being commonly used as an indicator for determination the genetic diversity, while Inter-spread sequence repeat technique ISSR analysis based on variation found in the regions between microsatellites it has been used in genetic fingerprinting gene tagging and detection of clonal variation [4,5]. This technique which involved amplification of DNA segment present in between two identical microsatellite repeat regions by addition the oriental in opposite direction with suitable distances ISSR method has been reported produce more complex markers patterns than the RAPD markers In addition, ISSR method are more reproducible than RAPD method Because ISSR primers designed to anneal temperature to a microsatellite sequences are long than RAPD primers, allowing higher annealing temperature to be used.…”

Section: Introductionmentioning

confidence: 99%

Study genetic diversity between trichophyton rubrum isolates using ISSR and RAPD markers

Mezher¹,

Mohammad²,

Wahab³

et al. 2018

JoBRC

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The present study included detection of genetic variability, and identification of genetic relationship and finding a fingerprint of the ten clinical isolates related to Trichophyton rubrum using RAPD and ISSR markers. The experiment was carried out and the results performed using six primer of the RAPD markers these primers showed 239 amplified band , out of these band 90 of them was considered as a main band, and 149 was Polymorphic band , the largest number of bands was 30 in the isolate TR6 and less number of bands in the isolate TR7. The results clear the value of genetic diversity based on RAPD analysis the lowest value of genetic diversity (0.13005) between the isolate TR3 and TR9while the highest genetic diversity (0.55941) was between the isolates TR5 and TR8. The analysis of the relationship shows that there are three main groups: the first group include isolate TR8, while the second group included three isolates are isolate TR2, isolate TR10 and isolates TR3, The third group included three subgroups included the first isolate TR1,isolates TR4 and second isolate TR3 and isolate TR9 and the third included the isolate TR5 and isolates TR6.The results of ISSR experiments: the use six a primers in the of the ISSR showed 192 bands, in the isolate of Trichophyton rubrum, two of these primers showed monomorphic bands (in number and location) and six primers showed monomorphic and polymorphic bands, while one showed only polymorphic bands among Trichophyton rubrum isolates. And the largest number of bands was 24 in the TR5 and less number of bands 16 in isolate TR3 and isolate TR8 were finding DNA fingerprint to isolate TR1, isolate TR5, and isolate TR3. The ISSR markers showed lowest genetic polymorphism was (0.05561) between the isolates TR2 and isolate TR7 and the largest genetic distance was (0.40501) between the isolate TR4 and isolate TR8. The analysis of the relationship of genetic showed that there are three groups key first included isolates and only one isolate TR8 and the second involving isolates TR2 and isolate TR7, isolate TR4 and isolate TR3, while the group included three sub-groups, the first included isolate TR1 and isolate TR6, and the second involving isolate TR5, isolate TR10 and isolate TR9. The results confirmed the efficiency markers of the RAPD and ISSR in contrast, find a genetic fingerprint to the isolates of Trichophyton rubrum, and these markers differ in mechanical detection contrast, and coverage is Genome. Therefore Complementary to each other, although the ISSR markers were more efficient in terms of the number of binding sites in each type of the isolates of Trichophyton rubrum and so can the initiator of the discovery of a much larger area from Genome.

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Molecular identification of clinical Candida isolates by simple and randomly amplified polymorphic DNA-PCR

Cited by 21 publications

References 18 publications

Update of the list of QPS‐recommended biological agents intentionally added to food or feed as notified to EFSA 9: suitability of taxonomic units notified to EFSA until September 2018

Update of the list of QPS‐recommended biological agents intentionally added to food or feed as notified to EFSA 9: suitability of taxonomic units notified to EFSA until September 2018

Synergistic effect of tea tree oil on fungi causing vaginal thrush in pregnant women

Study genetic diversity between trichophyton rubrum isolates using ISSR and RAPD markers

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