Molecular identification of coliform bacteria from colicky breastfed infants

Savino, Francesco; Cordisco, Lisa; Tarasco, Valentina; Calabrese, R.; Palumeri, E; Matteuzzi, Diego

doi:10.1111/j.1651-2227.2009.01419.x

Cited by 127 publications

(141 citation statements)

References 28 publications

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“…The clinical significance of this is now recognized by its association with nosocomial infections in cohorts of hospitalized patients, including children and neonates. [8][9][10] KPC-producing K. pneumoniae are responsible for higher mortality as compared to non-KPC producers. 11 We observed three isolates of K. pneumoniae that were resistant to colistin and 10 isolates showed resistance to tigecyclin, while none of K. oxytoca showed any resistance to these two antibiotics.…”

Section: S60 Discussionmentioning

confidence: 99%

Klebsiella oxytoca: An emerging pathogen?

Singh

Cariappa

Kaur³

2016

Medical Journal Armed Forces India

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Section: S60 Discussionmentioning

confidence: 99%

Klebsiella oxytoca: An emerging pathogen?

Singh

Cariappa

Kaur³

2016

Medical Journal Armed Forces India

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“…Excessive intestinal gas production seems to be one of the causes of abdominal discomfort in breastfed infants with colic. The role of gut microbiota in formula-fed infants has not been explored yet and could be responsible for intra-intestinal gas load (6).…”

Section: Discussionmentioning

confidence: 99%

“…Sample size was calculated based on a difference between groups of 1.6 log10 cell/g faeces, according to the previous literature (6).…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, colicky infants present with higher counts of anaerobic Gram-negative bacteria and lower counts of intestinal lactobacilli than infants without colic (4,5). Additional proof of the link between the gut microbiota and infantile colic derives from a study in which gas-forming coliforms were prevalent in infants with colic but not in infants without colic (6). Investigations based on microarrays revealed that infants with colic in the first weeks of life showed a higher microbiota homogeneity and instability than control infants (7,8).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of formula‐fed infants with and without colic revealed significant differences in total bacteria, Enterobacteriaceae and faecal ammonia

Savino¹,

Quartieri

Marco³

et al. 2016

Acta Paediatrica

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Aim: This study compared the faecal microbial composition of formula-fed infants who did and did not have colic.Methods: Faecal samples from formula-fed infants under 16 weeks of age with (n = 38) and without (n = 39) colic were collected at Department of Pediatrics in Turin, Italy, between February 2014 and October 2015. The pH and faecal ammonia were determined and total bacteria, bifidobacteria, lactic acid bacteria and coliforms were quantified by fluorescent in situ hybridisation (FISH).Results: Faecal ammonia was significantly higher in the colicky infants than in the controls (483 vs. 216 lg/g, p < 0.05). The FISH counts of total bacteria were lower in colicky infants (1.8E10 AE 1.5E10) than in the controls (3.4E10 AE 3.0E10) (p < 0.05). The relative abundance of coliform bacteria was significantly higher in colicky infants (p < 0.05). No differences were observed for the bifidobacteria and lactic acid bacteria counts between the two groups.Conclusion: Our comparison of formula-fed infants with and without colic revealed significant differences in total bacteria, Enterobacteriaceae and faecal ammonia. This study provides the stimulus for further studies of the gut microbiome, using new methods of analysis such as 16S metagenomics sequencing in order to lead to more tailored dietary approaches.

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“…Recently a low amount of lactobacilli and an increased amount of coliform bacteria in the intestinal microbiota have been reported as a possible cause of gut dysmotility and increasing of gas production (6). Higher levels of ghrelin and motilin were found in infants affected by colic, even though further studies are required to clarify their role in infantile colic (7).…”

Section: Introductionmentioning

confidence: 94%

Infantile colic treatment: new prospects

Savino

Marco

Ceratto

2013

Preprint

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Infantile colic is a common disturbance occurring in the first three months of life. It is a benign condition and one of the main causes of pediatric consultation in the early part of life because of its great impact on family life.Some pediatricians are prone to undervalue this issue and there is no consensus concerning management and treatment.In literature are growing evidences about the effectiveness of dietary, pharmacological, complementary and behavioral therapies as options for the management of infantile colic.Dietary approach is based on the avoidance of cow's milk proteins in breast-feeding mothers and bottle-fed infants. Currently pharmacological agents over the counter or by prescription are not recommended because of reported side effects. There is limited evidence supporting the use of complementary and alternative treatments (herbal supplements, manipulative approach and acupuncture) or behavioral modification interventions.Recent studies have partially clarified the pathogenesis of this disturb and so new treatments, such as probiotics, have been proposed.Further investigations are needed in order to provide evidence-based guidelines.

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Molecular identification of coliform bacteria from colicky breastfed infants

Cited by 127 publications

References 28 publications

Klebsiella oxytoca: An emerging pathogen?

Klebsiella oxytoca: An emerging pathogen?

Comparison of formula‐fed infants with and without colic revealed significant differences in total bacteria, Enterobacteriaceae and faecal ammonia

Infantile colic treatment: new prospects

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