Molecular Identification of Endophytic Fungi from Banana Leaves (Musa spp.)

Zakaria, Latiffah; Aziz, Wan Nuraini Wan

doi:10.21315/tlsr2018.29.2.14

Cited by 40 publications

(23 citation statements)

References 36 publications

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“…Each sample of banana roots was observed with a significant difference in the community composition and species abundance. Irrespective of the cultivars and locations, Proteobacteria and Actinobacteria were consistently enriched in the roots of both symptomatic and non-symptomatic samples, as observed by other researchers for the plant species of banana, rice, maize, wheat, and sugar cane [22][23][24], which are generally known for their good response to labile carbon sources with the fast growth of the plants [25,26]. Taken together, our results reveal that Proteobacteria and Actinobacteria are adapted to banana roots.…”

Section: Discussionsupporting

confidence: 86%

Metagenomic Insights of the Root Colonizing Microbiome Associated with Symptomatic and Non-Symptomatic Bananas in Fusarium Wilt Infected Fields

Kaushal

Mahuku

Swennen

2020

Plants

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Plants tissues are colonized by diverse communities of microorganisms called endophytes. They are key determinants of plant production and health, for example by facilitating nutrient exchanges or limiting disease development. Endophytic communities of banana plants have not been studied until very recently, and their potential role in disease development has not been explored so far. Roots from symptomatic and non-symptomatic banana plants were sampled from fields infected by Fusarium oxysporum f.sp. cubense race 1. The goal was to compare the endophytic microbiota between symptomatic and non-symptomatic plants through high throughput sequencing of 16s rDNA and shotgun metagenome sequencing. The results revealed that the endophytic root microbiome in bananas is dominated by Proteobacteria and Bacteroidetes followed to a lesser extent by Actinobacteria. The development of disease greatly impacted the endophytic microbial communities. For example, Flavobacteriales abundance was correlated with symptom development.

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Section: Discussionsupporting

confidence: 86%

Metagenomic Insights of the Root Colonizing Microbiome Associated with Symptomatic and Non-Symptomatic Bananas in Fusarium Wilt Infected Fields

Kaushal

Mahuku

Swennen

2020

Plants

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“…The genera found in the Musa seeds were largely similar to previous studies of Musa endophytes from roots and leaves ( Sikora et al, 2008 ; Wang et al, 2014 ; Zakaria et al, 2016 ; Zakaria and Aziz, 2018 ), as well as other tropical tree endophytes, such as from cacao branches ( Rubini et al, 2005 ) and rubber leaves ( Vaz et al, 2018 ) and tropical orchid roots ( Bayman and Otero, 2007 ). The most commonly found genera, Lasiodiplodia , Fusarium , and Aspergillus , are all ubiquitous in both endophytic and other contexts.…”

Section: Discussionsupporting

confidence: 84%

Seed Banks as Incidental Fungi Banks: Fungal Endophyte Diversity in Stored Seeds of Banana Wild Relatives

et al. 2021

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Seed banks were first established to conserve crop genetic diversity, but seed banking has more recently been extended to wild plants, particularly crop wild relatives (CWRs) (e.g., by the Millennium Seed Bank (MSB), Royal Botanic Gardens Kew). CWRs have been recognised as potential reservoirs of beneficial traits for our domesticated crops, and with mounting evidence of the importance of the microbiome to organismal health, it follows that the microbial communities of wild relatives could also be a valuable resource for crop resilience to environmental and pathogenic threats. Endophytic fungi reside asymptomatically inside all plant tissues and have been found to confer advantages to their plant host. Preserving the natural microbial diversity of plants could therefore represent an important secondary conservation role of seed banks. At the same time, species that are reported as endophytes may also be latent pathogens. We explored the potential of the MSB as an incidental fungal endophyte bank by assessing diversity of fungi inside stored seeds. Using banana CWRs in the genus Musa as a case-study, we sequenced an extended ITS-LSU fragment in order to delimit operational taxonomic units (OTUs) and used a similarity and phylogenetics approach for classification. Fungi were successfully detected inside just under one third of the seeds, with a few genera accounting for most of the OTUs–primarily Lasiodiplodia, Fusarium, and Aspergillus–while a large variety of rare OTUs from across the Ascomycota were isolated only once. Fusarium species were notably abundant–of significance in light of Fusarium wilt, a disease threatening global banana crops–and so were targeted for additional sequencing with the marker EF1α in order to delimit species and place them in a phylogeny of the genus. Endophyte community composition, diversity and abundance was significantly different across habitats, and we explored the relationship between community differences and seed germination/viability. Our results show that there is a previously neglected invisible fungal dimension to seed banking that could well have implications for the seed collection and storage procedures, and that collections such as the MSB are indeed a novel source of potentially useful fungal strains.

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“…were the most relatively abundant endophyte. Studies on EF community residing in banana plants were extensively studied by various authors [16–19]. However, role of EF from different agro-ecosystems in Enset (false banana) is yet to be revealed in details.…”

Section: Introductionmentioning

confidence: 99%

Endophytic fungal diversity isolated from different agro-ecosystem of Enset (Ensete ventericosum) in Gedeo zone, SNNPRS, Ethiopia

Chauhan

Aysa

2019

BMC Microbiol

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Background Endophytic Fungi (EF) are an underexplored group of microorganisms as only a few plants have been studied with regards to their community. Diversity of EF found in young and old leaves of Enset plant has not been well studied. We analyzed and compared the colonization frequency (CF), richness diversity and fungal communities of the EF inhabiting the young and old leaves of Enset plant from Southern region of Ethiopia. Standard methods were used for isolation and identification of endophytic species from Enset leaves. Results The study investigates the difference in quantity, in variety, in consistent pattern of community of EF along with different Enset varieties. A total number of 18 samples were analyzed and 108 morphospecies of EF were isolated and distributed among 17 genera. Aspergillus sp. and Penicillium sp. were the most common fungi reported in Enset plant. The largest numbers of EF isolates were observed in Maziya and Arkiya variety and the diversity index and species richness were found to be significant in Enset plant among these varieties. A high number of EF was isolated from old leaves in comparison to young leaves among all the varieties studied. Composition of EF at different altitudinal location also varied within each sites. Conclusion Isolation, characterization and distribution of the EF from Enset plant is the first approach that has been conducted in the developing country like Ethiopia. The findings of the present study show that the Enset agro-forestry system produces potential variability in the colonization, richness diversity and composition of EF in Enset plants. The assemblage of EF in healthy tissues of Enset plants may indicate that some of the fungi are possible latent pathogens and some may become saprophytic. Electronic supplementary material The online version of this article (10.1186/s12866-019-1547-y) contains supplementary material, which is available to authorized users.

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Molecular Identification of Endophytic Fungi from Banana Leaves (Musa spp.)

Cited by 40 publications

References 36 publications

Metagenomic Insights of the Root Colonizing Microbiome Associated with Symptomatic and Non-Symptomatic Bananas in Fusarium Wilt Infected Fields

Metagenomic Insights of the Root Colonizing Microbiome Associated with Symptomatic and Non-Symptomatic Bananas in Fusarium Wilt Infected Fields

Seed Banks as Incidental Fungi Banks: Fungal Endophyte Diversity in Stored Seeds of Banana Wild Relatives

Endophytic fungal diversity isolated from different agro-ecosystem of Enset (Ensete ventericosum) in Gedeo zone, SNNPRS, Ethiopia

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