Molecular identification of hemoparasites in ixodid ticks in Iran

Shiri, Aioub; Kheirandish, Farnaz; Sazmand, Alireza; Kayedi, Mohammad Hassan; Hosseini-Chegeni, Asadollah

doi:10.1016/j.vprsr.2023.100967

Cited by 2 publications

(1 citation statement)

References 48 publications

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“…The rst documented case of ovine theileriosis in India occurred at a government sheep farm in Kurukappi, Madras state (Mamatha et al 2017). Subsequent reports on ovine theileriosis in India have been documented by Nangru et al, (2023), Hassan et al, (2024) and Shiri et al, (2024). Among Theileria species affecting small ruminants, Theileria uilenbergi, Theileria lestoquardi, and Theileria luwenshuni are characterized by high pathogenicity (Ulucesme et al 2023), while Theileria separate, Theileria ovis, and Theileria recondita are considered non-infective or mildly infective species (Razmi et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Cross-sectional study on prevalence of theileriosis in Kendrapada sheep, Odisha

Mohanty,

Acharya,

Mishra

et al. 2024

Preprint

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Ovine theileriosis represents a significant threat to both animal welfare and global agricultural productivity, particularly impacting economically disadvantaged farmers. Vector-borne haemoparasitic diseases like theileriosis have a profound effect on the health of livestock, leading to increased illness and mortality, especially in the coastal regions of Odisha. This research aimed to investigate and comprehend the prevalence, clinical manifestations, hematological and biochemical parameters, as well as molecular aspects of theileriosis in sheep from Kendrapada. Among 148 blood samples collected from Kendrapada sheep across four blocks, 13% tested positive for theileriosis, indicating a notable prevalence. The prevalence rate was highest among sheep aged 1–4 years (19%), followed by those younger than 1 year (17.9%) and older than 4 years (13.3%). Female sheep exhibited a higher prevalence (19%) compared to males (16%). Block-wise prevalence was highest in Aul (27.9%), followed by Marshaghai (19.4%), Kendrapada (16.1%), and Derabish (5.88%). Hematological analysis revealed deviations in parameters such as hemoglobin, total erythrocyte count, total leukocyte count, and mean corpuscular volume, while packed cell volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, and differential leukocyte count remained within normal ranges. Significant differences (P < 0.05) were observed in various parameters between Theileria-positive and negative sheep. However, there were no substantial variations noted among different age groups and genders of Theileria-positive sheep. PCR analysis identified an overall incidence of theileriosis at 17.57% in the study population, with sequences closely resembling Theileria luwenshuni isolates. This study confirms the high variability of the 18S small subunit rRNA gene among Theileria spp.

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Section: Introductionmentioning

confidence: 99%

Cross-sectional study on prevalence of theileriosis in Kendrapada sheep, Odisha

Mohanty,

Acharya,

Mishra

et al. 2024

Preprint

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Investigation of Babesia spp. and Theileria spp. in ticks from Western China and identification of a novel genotype of Babesia caballi

Zhang,

Gao

et al. 2024

BMC Vet Res

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Babesia spp. and Theileria spp. are tick-borne protozoan parasites with veterinary importance. In China, epidemiological and genetic investigations on many Babesia and Theileria species were still absent in many areas and many tick species. From Aug 2021 to May 2023, 645 ticks were collected from the body surface of domestic animals (camels, goats, sheep, and cattle) using tweezers in seven counties in three provinces including Xinjiang (Qitai, Mulei, Hutubi, and Shihezi counties), Chongqing (Youyang and Yunyang counties), and Qinghai (Huangzhong county). Three tick species were morphologically and molecularly identified (334 Hyalomma asiaticum from Xinjiang, 245 Rhipicephalus microplus from Chongqing, and 66 Haemaphysalis qinghaiensis from Qinghai). A total of three Babesia species and two Theileria species were detected targeting the 18S gene. The COI and cytb sequences were also recovered from Babesia strains for further identification. In R. microplus from Chongqing, Babesia bigemina, the agent of bovine babesiosis, was detected. Notably, in H. asiaticum ticks from Xinjiang, a putative novel genotype of Babesia caballi was identified (0.90%, 3/334), whose COI and cytb genes have as low as 85.82% and 90.64–90.91% nucleotide identities to currently available sequences. It is noteworthy whether the sequence differences of its cytb contribute to the drug resistance of this variant due to the involvement of cytb in the drug resistance of Babesia. In addition, Theileria orientalis and Theileria annulata were detected in R. microplus from Chongqing (12.20%, 31/245) and H. asiaticum from Xinjiang (1.50%, 5/334), respectively. These results suggest that these protozoan parasites may be circulating in domestic animals in these areas. The pathogenicity of the novel genotype of B. caballi also warrants further investigation.

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Molecular identification of hemoparasites in ixodid ticks in Iran

Cited by 2 publications

References 48 publications

Cross-sectional study on prevalence of theileriosis in Kendrapada sheep, Odisha

Cross-sectional study on prevalence of theileriosis in Kendrapada sheep, Odisha

Investigation of Babesia spp. and Theileria spp. in ticks from Western China and identification of a novel genotype of Babesia caballi

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