Molecular Identification of<i>Hypericum perforatum</i>by PCR Amplification of the ITS and 5.8S rDNA Region

Howard, Caroline; Bremner, Paul; Fowler, Mark R.; Isodo, Belinda; Scott, Nigel W.; Slater, Robert

doi:10.1055/s-0029-1185397

Cited by 28 publications

(30 citation statements)

References 18 publications

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“…According to Howard et al ., the nuclear ribosomal Internal transcribed spacer (ITS1 and ITS2) DNA region is the most suitable ‘‘barcode’’ for primer creation (through PCR), plant species distinguishing and detection of adulterants. Many DNA barcoding studies on Hypericum species identification have been conducted including those of Crockett et al ., Park and Kim, Howard et al ., Pilepic et al . and Costa et al …”

Section: Dna Barcodingmentioning

confidence: 99%

Quality control of Hypericum perforatum L. analytical challenges and recent progress

Agapouda

Booker

Kiss

et al. 2019

Journal of Pharmacy and Pharmacology

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Objectives The most widely applied qualitative and quantitative analytical methods in the quality control of Hypericum perforatum extracts will be reviewed, including routine analytical tools and most modern approaches. Key findings Biologically active components of H. perforatum are chemically diverse; therefore, different chromatographic and detection methods are required for the comprehensive analysis of St. John's wort extracts. Naphthodianthrones, phloroglucinols and flavonoids are the most widely analysed metabolites of this plant. For routine quality control, detection of major compounds belonging to these groups seems to be sufficient; however, closer characterization requires the detection of minor compounds as well. Conclusions TLC and HPTLC are basic methods in the routine analysis, whereas HPLC‐DAD is the most widely applied method for quantitative analysis due to its versatility. LC‐MS is gaining importance in pharmacokinetic studies due to its sensitivity. Modern approaches, such as DNA barcoding, NIRS and NMR metabolomics, may offer new possibilities for the more detailed characterization of secondary metabolite profile of H. perforatum extracts.

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Section: Dna Barcodingmentioning

confidence: 99%

Quality control of Hypericum perforatum L. analytical challenges and recent progress

Agapouda

Booker

Kiss

et al. 2019

Journal of Pharmacy and Pharmacology

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“…The working assay was used to test DNA extracted from commercial preparations of St. John’s Wort sold in capsule or tablet form, produced by companies A, B and C. The three DNA samples were previously confirmed by conventional PCR of the ITS regions [17]. DNA degradation and/or shearing was observed in the samples from companies A and B, as the 750-bp ITS region could not be amplified, while a smaller amplicon (160 bp) within this region was amplified.…”

Section: Resultsmentioning

confidence: 99%

PlantID – DNA-based identification of multiple medicinal plants in complex mixtures

et al. 2012

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BackgroundAn efficient method for the identification of medicinal plant products is now a priority as the global demand increases. This study aims to develop a DNA-based method for the identification and authentication of plant species that can be implemented in the industry to aid compliance with regulations, based upon the economically important Hypericum perforatum L. (St John’s Wort or Guan ye Lian Qiao).MethodsThe ITS regions of several Hypericum species were analysed to identify the most divergent regions and PCR primers were designed to anneal specifically to these regions in the different Hypericum species. Candidate primers were selected such that the amplicon produced by each species-specific reaction differed in size. The use of fluorescently labelled primers enabled these products to be resolved by capillary electrophoresis.ResultsFour closely related Hypericum species were detected simultaneously and independently in one reaction. Each species could be identified individually and in any combination. The introduction of three more closely related species to the test had no effect on the results. Highly processed commercial plant material was identified, despite the potential complications of DNA degradation in such samples.ConclusionThis technique can detect the presence of an expected plant material and adulterant materials in one reaction. The method could be simply applied to other medicinal plants and their problem adulterants.

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“…For instance, sequences of internal transcribed spacers (ITS) encoded in the nuclear genome and rbcL and trnL-trnF encoded in the plastid genome have been used to discriminate or identify plants (Artyukova et al, 2005;Kojoma, 2002;Newmaster et al, 2006). Medicinal plants, in particular, such as Hypericum perforatum and Lonicera japonica have been discriminated efficiently using ITS sequences (Howard et al, 2009;Peng et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Discrimination of Kalopanax pictus from its varieties and other ‘Kalopanacis Cortex’ plants by multiplex polymerase chain reaction (PCR)

Doh

Yang

et al. 2011

Genes Genom

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Kalopanacis Cortex' (KC) is an important medicinal material prescribed in Korea for the treatment of various ailments such as paralysis, arthritis, and rheumatism. In Korea, KC is defined as the dried stem bark of Kalopanax pictus. However, the stem barks of Zanthoxylum ailanthoides and Erythrina plants such as E. variegata have also been described as KC and prescribed for the same diseases. Since the pharmacological activities and contents of compounds isolated from the stem barks of these KC plants are obviously different, KC from K. pictus should be clearly discriminated from other stem bark in the best interest of public health. This study sequenced the internal transcribed spacers (ITS) of 52 samples of the KC or KC plants collected from Korea, China, and Vietnam. On the basis of different nucleotide sequences of specific ITS regions among the KC plants, the primer set KP F2/KC R1 was designed to amplify a 398-bp DNA marker for discriminating K. pictus from its varieties and from other KC plants. This primer set, along with the primer sets ZR F1/ZR R4, KP F1/EV R2, and KPF1/KC R1, was successfully amplified with the DNA markers of the Z. ailanthoides, E. variegata, and K. pictus taxa, respectively. The multiplex polymerase chain reaction method developed here not only discriminates the stem bark of K. pictus from stem barks of other KC plants but also identifies KC plants that supply KC in a single process.

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Molecular Identification ofHypericum perforatumby PCR Amplification of the ITS and 5.8S rDNA Region

Cited by 28 publications

References 18 publications

Quality control of Hypericum perforatum L. analytical challenges and recent progress

Quality control of Hypericum perforatum L. analytical challenges and recent progress

PlantID – DNA-based identification of multiple medicinal plants in complex mixtures

Discrimination of Kalopanax pictus from its varieties and other ‘Kalopanacis Cortex’ plants by multiplex polymerase chain reaction (PCR)

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