Molecular Identification of IgE-Dependent Histamine-Releasing Factor as a B Cell Growth Factor

Kang, Hyung Sik; Lee, Min Ju; Song, Hyun Keun; Han, Seung Hyun; Kim, Yong Man; Im, Joo Young; Choi, In Pyo

doi:10.4049/jimmunol.166.11.6545

Cited by 71 publications

(46 citation statements)

References 34 publications

(40 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An example included IgE-dependent histamine-releasing factor, a B cell growth factor and basophil activator. This basophil activator is expressed in myeloid cells and is involved in delayed-type allergic responses (39). Connexin 43, the most abundant of all SAGE tags in this study, may also indicate a connection with macrophages, since activated macrophages express this gap junction protein (40).…”

Section: Discussionmentioning

confidence: 99%

Serial Analysis of Gene Expression in Circulating γδ T Cell Subsets Defines Distinct Immunoregulatory Phenotypes and Unexpected Gene Expression Profiles

Meissner

Radke

Hedges

et al. 2003

The Journal of Immunology

View full text Add to dashboard Cite

Gene expression profiles were compared in circulating bovine GD3.5+ (CD8−) and GD3.5− (predominantly CD8+) γδ T cells using serial analysis of gene expression (SAGE). Approximately 20,000 SAGE tags were generated from each library. A comparison of the two libraries demonstrated 297 and 173 tags representing genes with 5-fold differential expression in GD3.5+ and GD3.5− γδ T cells, respectively. Consistent with their localization into sites of inflammation, GD3.5+ γδ T cells appeared transcriptionally and translationally more active than GD3.5− γδ cells. GD3.5− γδ T cells demonstrated higher expression of the cell proliferation inhibitor BAP 37, which was associated with their less activated gene expression phenotype. The immune regulatory and apoptosis-inducing molecule, galectin-1, was identified as a highly abundant molecule and was higher in GD3.5+γδ T cells. Surface molecules attributed to myeloid cells, such as CD14, CD68, and scavenger receptor-1, were identified in both populations. Furthermore, expression of B lymphocyte-induced maturation protein, a master regulator of B cell and myeloid cell differentiation, was identified by SAGE analysis and was confirmed at the RNA level to be selectively expressed in γδ T cells vs αβ T cells. These results provide new insights into the inherent differences between circulating γδ T cell subsets.

show abstract

Section: Discussionmentioning

confidence: 99%

Serial Analysis of Gene Expression in Circulating γδ T Cell Subsets Defines Distinct Immunoregulatory Phenotypes and Unexpected Gene Expression Profiles

Meissner

Radke

Hedges

et al. 2003

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…TCTP has been shown to trigger the release of histamine from eosinophils, basophils, and mast cells and was also found to be a potent stimulus for interleukin-4 and interleukin-8 production (19,20). In addition, TCTP was shown to have a B-cell stimulating activity (21). Under allergic conditions, TCTP was detected in nasal and skin blister fluids from patients with late phase allergic inflammation (22) and in bronchoalveolar lavages from patients with idiopathic eosinophilic pneumonia (23).…”

Section: Tsap6mentioning

confidence: 99%

“…The high levels of constitutive secretion of endogenous TCTP and the low transfection efficiency obtained may account for the absence of augmented secretion of endogenous TCTP by TSAP6 in this cell line. Finally, as TCTP was originally identified as a secreted factor in hematopoietic cells (18,21), we addressed whether overexpression of TSAP6 could enhance the secretion of TCTP in the erythroleukemia cell line K562. Similar to the effects observed in 293T cells, overexpression of HA-TSAP6 increased the levels of both endogenous and exogenous TCTP in the supernatants of K562 cells (Fig.…”

Section: Tsap6 Facilitates the Secretion Of Tctp 46108mentioning

confidence: 99%

TSAP6 Facilitates the Secretion of Translationally Controlled Tumor Protein/Histamine-releasing Factor via a Nonclassical Pathway

Amzallag

Passer

Allanic

et al. 2004

Journal of Biological Chemistry

192

184

View full text Add to dashboard Cite

Translationally controlled tumor protein (TCTP) is cytoplasmic and structurally related to guanine-nucleotide free chaperones. TCTP (also called histamine-releasing factor) has been described previously as a secreted protein that participates in inflammatory responses by promoting the release of histamine. How TCTP is eventually exported out of the cell to promote such activities is unknown. Here we show that TCTP secretion was insensitive to either brefeldin A or monensin, suggesting that it proceeds via an endoplasmic reticulum/Golgi-independent or nonclassical pathway. Moreover, our analyses also suggest that secreted TCTP originates from pre-existing pools. TSAP6, a p53-inducible 5-6 transmembrane protein, was found to interact with TCTP in a yeast two-hybrid hunt. GST pull down assays confirmed their direct interaction, and immunofluorescence analysis revealed their partial co-distribution to vesicular-like structures at the plasma membrane and around the nucleus. Functionally, the overexpression of TSAP6 consistently leads to enhanced secretion of both endogenously and exogenously expressed TCTP. Finally, we found TCTP in preparations of small secreted vesicles called exosomes, which have been suggested as a possible pathway for nonclassical secretion. Overexpression of TSAP6 also increased TCTP levels in exosome preparations. Altogether, these data identify a novel role for TSAP6 in the export of TCTP and indicate that this multipass membrane protein could have a general role in the regulation of vesicular trafficking and secretion.

show abstract

“…More recently, a protein with B cell stimulatory activity isolated from the culture supernatants of the murine erythroleukemia cell line LK1 was identified as murine TCTP/HRF (9). Incubation of murine splenic B cells with cloned recombinant murine HRF (mHRF) was found to stimulate proliferation, increase MHC class II expression, and up-regulate the message for the cytokines IL-1, IL-6, and IL-10.…”

Section: Inhibition Of Cytokine Gene Transcription By the Human Recommentioning

confidence: 99%

“…To investigate the in vivo effects of mHRF on B cell function, BALB/c mice were injected i.m. with mHRF, and the effects on B cell Ig production and proliferation were investigated (9). Mice injected with mHRF demonstrated increased serum Ig production as well as enhanced proliferation and Ig production of their anti-CD40-stimulated B cells.…”

Section: Inhibition Of Cytokine Gene Transcription By the Human Recommentioning

confidence: 99%

Inhibition of Cytokine Gene Transcription by the Human Recombinant Histamine-Releasing Factor in Human T Lymphocytes

Vonakis¹,

Sora²,

Langdon³

et al. 2003

The Journal of Immunology

View full text Add to dashboard Cite

Human recombinant histamine-releasing factor (HrHRF) preincubation enhances the secretion of histamine, IL-4, and IL-13 from FcεRI-stimulated human basophils. In GM-CSF-primed human eosinophils, HrHRF increases IL-8 production. Our recent experiments were designed to evaluate the effects of HrHRF on human T cell cytokine production. Purified T cells were preincubated with GST-tagged HrHRF, followed by stimulation with PMA and A23187 overnight. A partial inhibition of IL-2 and IL-13 production (30 and 75%, respectively) was detected compared with that in cells treated with PMA/A23187 alone. However, the production of IFN-γ was similar in PMA/A23187 stimulated cells with or without HrHRF. The inhibition of cytokine protein production was dose dependent and specific to the HrHRF portion of GST-HrHRF. The inhibition was not due to endotoxin, since preincubation with polymyxin B and HrHRF gave similar results to that with HrHRF alone. The same pattern and specificity of cytokine regulation were replicated in the Jurkat T cell line as for primary T cells. The PMA/A23187-stimulated activity of a proximal promoter IL-13, IL-4, or IL-2 luciferase construct transfected into Jurkat cells was partially inhibited (60, 32, or 70%, respectively) upon GST-HrHRF preincubation, suggesting that HrHRF functions to inhibit cytokine production in Jurkat cells by preventing gene transcription. The inhibition of IL-2 promoter activation was specific to the HrHRF portion of GST-HrHRF. We conclude that HrHRF, in addition to functioning as a histamine-releasing factor, can differentially modulate the secretion of cytokines from human basophils, eosinophils, T cells, and murine B cells, suggesting that it may induce a complex array of responses at sites of allergic inflammation.

show abstract

Molecular Identification of IgE-Dependent Histamine-Releasing Factor as a B Cell Growth Factor

Cited by 71 publications

References 34 publications

Serial Analysis of Gene Expression in Circulating γδ T Cell Subsets Defines Distinct Immunoregulatory Phenotypes and Unexpected Gene Expression Profiles

Serial Analysis of Gene Expression in Circulating γδ T Cell Subsets Defines Distinct Immunoregulatory Phenotypes and Unexpected Gene Expression Profiles

TSAP6 Facilitates the Secretion of Translationally Controlled Tumor Protein/Histamine-releasing Factor via a Nonclassical Pathway

Inhibition of Cytokine Gene Transcription by the Human Recombinant Histamine-Releasing Factor in Human T Lymphocytes

Contact Info

Product

Resources

About