Molecular identification of Korean ginseng by amplification refractory mutation system-PCR

Park, Min Ju; Kim, Myung Kyum; In, Jun Gyo; Yang, Deok Chun

doi:10.1016/j.foodres.2005.11.004

Cited by 46 publications

(36 citation statements)

References 20 publications

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“…2). Reported ITSs sequences of P. notoginseng, P. japonicus, P. major, P. trifolius P. ginseng, P. quinquefolius (Wen and Zimmer 1996;Ngan et al 1999) as well as those of three accessions (Jakyung, Chungkyung, and Hwangsook) and two cultivars (Yunpoong and Chunpoong) of ginseng (Park et al 2006) were consistent with our results. It has been emphasized that it is necessary to classify a specific region of the various ginseng cultivars that can then be used to differentiate between domestic and foreign ginseng when exporting Korean ginseng, but information on such regions of P. ginseng that include all accessions and cultivars is still poor.…”

Section: Resultssupporting

confidence: 90%

“…It has been emphasized that it is necessary to classify a specific region of the various ginseng cultivars that can then be used to differentiate between domestic and foreign ginseng when exporting Korean ginseng, but information on such regions of P. ginseng that include all accessions and cultivars is still poor. According to the recent results described by Yang et al (2001) and Park et al (2006), the sequences of ITS1 and ITS2 were almost identical for every accession and cultivar within P. ginseng, but the sequence of 5.8S rDNA for the Hwangsook accession was different from the sequences of 5.8S rDNA for the others with respect to 1 bp at nucleotide position 14. In our results, the 5.8S rDNA sequence of the Hwangsook accession as well as those of two cultivars (Gopoong and Kumpoong) differed from those of other ginsengs at nucleotide position 14 (region labeled ''B'' in Fig.…”

Section: Resultsmentioning

confidence: 73%

“…Among these, three cultivars-Chunpoong, Gopoong, and Kumpoong-are suitable for generating ''red ginseng'' (steamed ginseng roots) because they have good root shapes. Recently, foreign ginseng cultivars have been passed off as Korean ginseng, which is an illegal practice and one that has caused serious social problems, because Korean ginseng cultivars fetch a much greater market value than those cultivated in other countries (Park et al 2006). Therefore, a scientific method of identifying ginseng cultivars at the molecular level is required, so that the commercial interests of consumers can be protected.…”

Section: Introductionmentioning

confidence: 99%

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Molecular authentication of ginseng cultivars by comparison of internal transcribed spacer and 5.8S rDNA sequences

Kim

Bang

et al. 2007

Plant Biotechnol Rep

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Molecular authentication among three Panax species and within cultivars and accessions of P. ginseng was investigated using the DNA sequence in the ribosomal ITS1-5.8S-ITS2 region. Four single-nucleotide polymorphisms were identified between P. ginseng and other Panax species. In the electrophoresis profile, obtained after digestion with the enzyme TaqI, three fingerprinting patterns were obtained from cultivars and accessions of Panax species. Consequently, this authentication procedure based upon the restriction fragment length polymorphism in the ribosomal ITS1-5.8S-ITS2 region can now be utilized to differentiate these Panax species as well as major Korean cultivars such as Gopoong and Kumpoong from other cultivars and accessions in Panax species at the DNA level.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

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Molecular authentication of ginseng cultivars by comparison of internal transcribed spacer and 5.8S rDNA sequences

Kim

Bang

et al. 2007

Plant Biotechnol Rep

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“…Magnoliae Flos due to their morphological similarities, most of medicinal plant products in the markets are packaged of powders of slices and Processed things that no longer bear the original morphological features of the plants (Park et al, 2006;Jigden et al, 2010;Jin et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Molecular Authentication of Magnoliae Flos Using Robust SNP Marker Base on trnL-F and ndhF Region

Kim¹,

Noh²,

Yan³

et al. 2015

Korean Journal of Plant Resources

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-Magnoliae Flos (Sini in Korean) is one of the most important oriental medicinal plants. In the Korean Herbal Pharmacopeia, the bud of the all species in Manolia denudate and Manolia genus were regarded as the botanical sources for 'Sini'. Most the dried bud of Manolia denudata, Manolia biondii and Manolia sprengeri were used as 'Xin-yi' in China. Therefore, the purpose of this study was to determine and compare the 'Magnolia' species, four species including Manolia denudata, M. biondii, M. liliiflora and M. Kobus were analysis of sequencing data revealed DNA polymorphisms. The based on tRNA coding leucine/phenylalanine (trnL-F) and NADH-plastoquinone oxidoreductase subunit 5 (ndhF) sequences in chloroplast DNA. For the identification of 'Magnolia' species, polymerase chain reaction (PCR) analysis of chloroplast DNA regions such as ndhF have proven an appropriate method. A single nucleotide polymorphism (SNP) has been identified between genuine "Sini" and their fraudulent and misuse. Specific PCR primers were designed from this polymorphic site within the sequence data, and were used to detect true plants via multiplex PCR.

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“…DNA 마커를 이용한 인삼의 종 판별연구는 internal transcribed spacer (ITS)와 5.8S 부위의 염기서열 변이를 이 용한 방법 (Wen and Zimmer, 1996;Ngan et al, 1999;Park et al, 2006;Kim et al, 2007), 엽록체 DNA의 변 이를 이용한 PCR-RFLP (Choi and Wen, 2000), universal primer를 적용시킨 RAPD (Um et al, 2001, Shim et al, 2003Bang et al, 2004) AFLP (Ha et al, 2002), ISSR (Fig. 2).…”

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Analysis of Mitochondrial DNA Sequence and Molecular Marker Development for Identification of Panax Species

Jo¹,

Bang²,

Kim³

et al. 2013

Korean Journal of Medicinal Crop Science

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: This study describes the identification of Panax species using mitochondrial consensus primers. Initially, a total of thirty primers were tested in ten Korean ginseng cultivars and two foreign Panax species, P. quinquefolius and P. notoginseng. In the polymerase chain reaction (PCR) amplification results, three primers (cox1, nad1/2-3 and nad2/1-2) generated co-dominant polymorphic banding patterns discriminating Korean ginseng cultivars from P. quinquefolius and P. notoginseng. However, these primers could not generated polymorphisms among the Korean ginseng cultivars, and simply represented species-specific polymorphisms for P. quinquefolius and P. notoginseng. Primers PQ91 and PN418 were designed from the consensus sequence of nad1/2-3 region. Two banding patterns (A or B) were detected in PQ91. Korean ginseng cultivars and P. notoginseng shared the same banding pattern (A type) and P. quinquefolius was identified another banding pattern (B type). In the case of PN418, two banding patterns (A or B) were detected in the Korean ginseng cultivars and two foreign Panax species. Korean ginseng cultivars and P. quinquefolius shared the same banding pattern (A type) and P. notoginseng was identified another banding pattern (B type). The combination banding patterns of three Panax species, Korean ginseng cultivars (Panax ginseng C. A. Mey.), P. quinquefolius and P. notoginseng, was identified as 'AA', 'BA' and 'AB', respectively. Consequently, PQ91 and PN418 primer sets can be used to distinguish among Panax species.

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Molecular identification of Korean ginseng by amplification refractory mutation system-PCR

Cited by 46 publications

References 20 publications

Molecular authentication of ginseng cultivars by comparison of internal transcribed spacer and 5.8S rDNA sequences

Molecular authentication of ginseng cultivars by comparison of internal transcribed spacer and 5.8S rDNA sequences

Molecular Authentication of Magnoliae Flos Using Robust SNP Marker Base on trnL-F and ndhF Region

Analysis of Mitochondrial DNA Sequence and Molecular Marker Development for Identification of Panax Species

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