Molecular identification of Lavendula dentata L., Mentha longifolia (L.) Huds. and Mentha × piperita L. by DNA barcodes

Ahmed, Shawkat M.

doi:10.3329/bjpt.v25i2.39519

Cited by 6 publications

(4 citation statements)

References 11 publications

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“…Kalfagianni [ 67 ] ( trnH-psbA and ITS) points again to strongly heterogeneous results signifying probably high putative intraspecific variability, but possibly due to labeling issues the results may be incomparable and inconclusive. Moreover, the rbcL -based phylogeny by Ahmed [ 28 ] is clearly different, which might also be a consequence of sampling (bias).…”

Section: Discussionmentioning

confidence: 99%

“…The incongruences between evolutionary histories of nuclear and plastid DNA in the subtribe Menthinae are not surprising given the high frequency of interspecific hybridizations [ 123 ]. The low genetic differences found between mints using plastid markers [ 28 , 93 , 97 , 121 , 123 ] show that these markers on their own are not as good as nuclear markers to infer interspecific relationships between mints. Bunsawatt et al [ 93 ] explicitly mention that some parts of the trnL-trnF and the ITS phylogenies lack resolution.…”

Section: Discussionmentioning

confidence: 99%

“…Many authors (e.g., [25][26][27][28][29][30][31][32]) have already described the complex (morphological, chemical and molecular) diversity in mints. The complicated historical nomenclature (2630 names for Mentha on https://www.tropicos.org and https://www.catalogueoflife.org; accessed on 17 January 2021) strongly contrasts with the apparent simplicity presented by some authors nowadays.…”

Section: Introductionmentioning

confidence: 99%

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A Revised Phylogeny of the Mentha spicata Clade Reveals Cryptic Species

Heylen¹,

Debortoli²,

Marescaux³

et al. 2021

Plants

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The genus Mentha is taxonomically and phylogenetically challenging due to complex genomes, polyploidization and an extensive historical nomenclature, potentially hiding cryptic taxa. A straightforward interpretation of phylogenetic relationships within the section Mentha is further hindered by dominant but outdated concepts on historically identified hybrid taxa. Mentha spicata is traditionally considered to be of hybrid origin, but the evidence for this is weak. Here, we aim to understand the phylogenetic relationships within the section Mentha using large sample sizes and to revisit the hybrid status and identity of M. spicata. We show that two of three traditional species in the subsection Spicatae are polyphyletic, as is the subsection as a whole, while the real number of cryptic species was underestimated. Compared to previous studies we present a fundamentally different phylogeny, with a basal split between M. spicata s.s. and M. longifolia s.s. Cluster analyses of morphological and genotypic data demonstrate that there is a dissociation between morphologically and genotypically defined groups of samples. We did not find any evidence that M. spicata is of hybrid origin, and we conclude its taxonomic status should be revised. The combination of genetic and phenotypic information is essential when evaluating hyperdiverse taxonomic groups.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Revised Phylogeny of the Mentha spicata Clade Reveals Cryptic Species

Heylen¹,

Debortoli²,

Marescaux³

et al. 2021

Plants

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“…A similar report was revealed by Zabta et al (2014 that the rbcl region was more effective to identify and discriminate the species of Acacia and Abizia. DNA barcoding could be considered as a good approach for distinguishing and identifying the mint plants (Hameed 2018).…”

Section: Phylogenetic Analysis Of Isoberlinia Doka and Isoberliniamentioning

confidence: 99%

Morphological, anatomical and molecular characterisation of the leaves of Isoberlinia doka Craib and Stapf and Isoberlinia tomentosa (Harms) Craib and Stapf

Bello

Katsayal²,

Ambi³

et al. 2023

JOMPED

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Background: Isoberlinia (Craib and Stapf) is a genus with high economic and pharmacological values.Aim: This study aimed at establishing the morphological, anatomical and molecular characterisation of the leaves of I. doka and I. tomentosa, which were conducted for proper authentication.Setting: The leaves of I. doka and I. tomentosa were obtained from Shika, kaduna State, Nigeria.Method: Morphological and anatomical characters were determined according to standard procedures, while molecular identifications were performed using ribulose-1,5-bisphosphate carboxylase (rbcl) gene and internal transcribed spacer (ITS) DNA barcode’s region.Result: Morphological studies revealed similar features for both species except for the shiny leaves of I. doka and rough abaxial surfaces of I. tomentosa because of the presence of trichomes. Variations were observed in their epidermal features, stomatal index, stomata frequency, presence or absence of trichomes, trichomes frequency and their quantitative anatomical features. The quantity and quality of DNA measured at A260/280 ratio using nanodrop spectrophotometer were 29.1 ng/μL and 1.74 ng/μL for I. doka, respectively, while the I. tomentosa concentration and purity were 71.1 ng/μL and 1.85 ng/μL, respectively. Agarose gel electrophoresis revealed two DNA bands with 700 bp (rbcl) and 600 bp (ITS). The sequence analysis revealed maximum identity with National Centre for Biotechnology Information (NCBI) GeneBank Isoberlinia species. Evolutionary analysis supported the monophyletic origin of the genus Isoberlinia. The morphological and anatomical characters of I. doka and I. tomentosa leaves have provided a significant taxonomy tool for proper authentication of this plant.Conclusion: The findings ascertained that ITS and rbcl served as an improved and efficient tool for species identification of these studied species and could serve as potential DNA barcodes for these taxa.Contribution: This article suggests that further studies the on screening of these plants, for various pharmacological potentials, might be useful for new drug development.

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Species identification and germplasm conservation of origanum based on chloroplast genes

Furan

2023

Genet Resour Crop Evol

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Molecular identification of Lavendula dentata L., Mentha longifolia (L.) Huds. and Mentha × piperita L. by DNA barcodes

Cited by 6 publications

References 11 publications

A Revised Phylogeny of the Mentha spicata Clade Reveals Cryptic Species

A Revised Phylogeny of the Mentha spicata Clade Reveals Cryptic Species

Morphological, anatomical and molecular characterisation of the leaves of Isoberlinia doka Craib and Stapf and Isoberlinia tomentosa (Harms) Craib and Stapf

Species identification and germplasm conservation of origanum based on chloroplast genes

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