Molecular Identification of Mealybugs

Rebijith, K. B.; Asokan, R.; Kumar, Narendra

doi:10.1007/978-81-322-2677-2_5

Cited by 3 publications

(3 citation statements)

References 25 publications

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“…In animals, the COI gene is frequently used to identify species through the use of the polymerase chain reaction (PCR) technique and universal primers to facilitate amplification (Folmer et al, 1994). This gene sequence is always preserved within a species, and the rate of mutation is fast enough to distinguish between closely related species (Rebijith et al, 2016). For milkfish conservation and aquaculture development in Vietnam, it is important to gain a better understanding of the genetic variability in milkfish stocks.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of Chanos Chanos (Forsskål, 1775) from Natural Populations in Vietnam

Trang

Ngân

et al. 2022

vjas

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The genetic diversity of five natural populations of milkfish (Chanos chanos) collected in Nghe An, Quang Binh, Binh Dinh, Phu Yen, Khanh Hoa provinces in Vietnam was examined using COI gene sequence analysis. Twelve haplotypes were noted from a total of 50 sequences along with 12 variable sites and 6 parsimony informative sites. The Quang Binh milkfish population had the highest haplotype (0.889 ± 0.060) and nucleotide diversities (0.00301 ± 0.00049). Overall, haplotype and nucleotide diversities were 0.804 ± 0.036 and 0.00212 ± 0.00026, respectively. Genetic differentiation (FST) was high between the milkfish populations of Nghe An – Quang Binh (0.21744) and Nghe An - Phu Yen (0.26215). Haplotype network analysis indicated that milkfish populations shared common haplotypes and each population had its own private haplotypes. Population structure and demographic expansion were not evident for all populations except for Quang Binh. This is the first principal endeavor to understand genetic information of milkfish in Vietnam, thereby providing information for scientists, managers, and the general public to establish timely strategies to explore, protect, and develop milkfish genetic resources in the future.

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Section: Introductionmentioning

confidence: 99%

Genetic Diversity of Chanos Chanos (Forsskål, 1775) from Natural Populations in Vietnam

Trang

Ngân

et al. 2022

vjas

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“…They have been indistinguishable in many studies based on morphological characteristics and misidentified as P. rudis as a result [ 3 , 5 ]. Molecular techniques have been developed to overcome such challenges, and allow identification regardless of variables such as life stage, sex, and colour morph [ 22 ]. DNA barcoding in animal species uses short strands of DNA sequences which are frequently isolated from the mitochondrial cytochrome c oxidase I gene ( COI ), as it is widely considered the standard gene for such analyses.…”

Section: Introductionmentioning

confidence: 99%

Molecular identification of the potentially forensically relevant cluster fliesPollenia rudis(Fabricius) andPollenia vagabunda(Meigen) (Diptera: Polleniidae) — non-recorded species in Algeria

Taleb

Tail

Açikgöz

2021

Forensic Sciences Research

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Cluster flies are represented by the genus Pollenia Robineau-Desvoidy, 1830 of the family Polleniidae Brauer and Bergenstamm, 1889. Their larvae are known to be internal parasites or predators of earthworms. Herein, we report for the first time the occurrence of the cluster flies Pollenia rudis Fabricius, 1794 and Pollenia vagabunda (Meigen, 1826) (Diptera: Polleniidae) on carcasses in Algeria and identify them through DNA barcoding. A region of the mitochondrial cytochrome c oxidase I gene ( COI ) was amplified and sequenced. Genetic distances were determined. A phylogenetic tree was constructed with the maximum parsimony method using 10 000 bootstrap replicates. A total number of 157 adults of P. rudis were collected together with 325 adults of Pollenia vagabunda . The occurrence of Pollenia on animal carcasses does not seem to be correlated with a particular stage of decomposition. All the sequences were correctly identified using the BLASTn tool from the GenBank database and the BOLD identification engine. Intra- and interspecific sequence divergence values were less than 1% and greater than 3%, respectively. COI barcodes obtained from this study were robust enough to identify and distinguish unambiguously between P. rudis and P. vagabunda . In the tree-based analysis, the cluster flies were all assigned to their respective species separately from each other confirming the morphological identification. These results provide DNA barcodes that contribute to the growth of reference databases and allow fast and accurate identification.

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“…8 This sequence of genes is always conserved among individuals in the same species and the rate of mutation is fast enough to distinguish between species with close genetic relationships. 9 In this study, the mitochondrial COI gene sequence was used to identify species and genetic relationship analysis in Meretrix genus that were collected in some Northern coastal provinces in Vietnam.…”

Section: Introductionmentioning

confidence: 99%

Genetic relationship of asiatic hard clam populations collected in northern coastal provinces in vietnam based on mtDNA sequence analysis

Trang¹

2018

JAMB

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The genetic relationship of some Asiatic hard clam (Meretrix meretrix) based on mtDNA COI sequence analysis was investigated for populations collected in Thai Binh, Nam Dinh, Nghe An provinces in Vietnam. In addition, this research also targets at species identification based on COI sequences. In total of 59 sequences analyzed, 19 sequences belonged to Meretrix meretrix species with Gen Bank accession number DQ399399.1. 17 sequences of M. meretrix were used for genetic relationship analysis among 3 populations. In which, 6 polymorphic sites, 3 parsimony informative sites and 4 haplotypes observed for the COI gene. Moderately genetic population diversity was observed, overall haplotype and nucleotide diversity were 0.476±0.233 and 0.00151±0.00069, respectively. Generally, genetic differentiation (F ST ) (F ST < 0.15) was moderate. The genetic distance was rather low, which ranged from 0.001 (Thai Binh-NgheAn, Thai Binh-Nam Dinh populations) to 0.002 (Nam Dinh -Nghe An populations). The result of haplotype network constructing indicated that populations shared common haplotype and there was no specific isolation of the haplotypes of the populations. Hence, it showed M. meretrix populations had intimate genetic relationship. The result of phylogenic tree indicated that three M. meretrix populations (Thai Binh, Nam Dinh, Nghe An) had a very small or no genetic variation among populations.

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Molecular Identification of Mealybugs

Cited by 3 publications

References 25 publications

Genetic Diversity of Chanos Chanos (Forsskål, 1775) from Natural Populations in Vietnam

Genetic Diversity of Chanos Chanos (Forsskål, 1775) from Natural Populations in Vietnam

Molecular identification of the potentially forensically relevant cluster fliesPollenia rudis(Fabricius) andPollenia vagabunda(Meigen) (Diptera: Polleniidae) — non-recorded species in Algeria

Genetic relationship of asiatic hard clam populations collected in northern coastal provinces in vietnam based on mtDNA sequence analysis

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