Molecular identification of phytoplasmas associated with little leaf disease of brinjal in Andhra Pradesh and Telangana states of India

Srilatha, V.; Reddy, Panyam Karthik; Priya, Pasupuleti Reddy; Reddy, Madem Gurivi

doi:10.5958/2249-4677.2021.00004.9

Cited by 2 publications

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“…In Telangana; Tamil Nadu and Tirupati; Andhra Pradesh; New Delhi; Uttar Pradesh; Maharashtra and Chhattisgarh; Kerala; Mysore; and Dharwad, South Gujarat, the phytoplasmas associated with brinjal little leaf were identified as belonging to the 16SrVI group, mainly the 16SrVI-D subgroup [38][39][40][41][42]. However, in Andhra Pradesh, 16SrII-D phytoplasmas were also identified [43]. Kumar et al [25] reported little leaf disease associated with 16SrI phytoplasmas in the brinjal fields of Bihar.…”

Section: Discussionmentioning

confidence: 99%

Eggplant Little Leaf-Associated Phytoplasma Detection in Seedlings under Insect-Proof Conditions

Darabakula,

Mateeti,

Pacini

et al. 2024

IJPB

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Eggplant, or brinjal, is one of the most consumed and important tropical solanaceous vegetable crops grown worldwide. Little leaf is a disease associated with the presence of phytoplasmas especially widespread in brinjal in India. To clarify the epidemiology of this disease, a verification of its transmission through seeds to seedlings and their progeny derived from symptomatic mother plants was performed. Brinjal seeds field-collected in the Dharwad district of Karnataka State, India, were sowed in a greenhouse under insect-proof conditions. DNA was extracted from seedlings and their progeny and from symptomatic plant samples collected in the field. The first- and second-generation seedlings obtained *under these conditions were tested at various time points after germination by amplification of the 16S rRNA gene of phytoplasmas. The amplicons obtained were subjected to restriction fragment length polymorphism (RFLP) analysis and sequencing for the identification of detected phytoplasmas. Ribosomal groups 16SrI, 16SrII, 16SrIII, 16SrV, 16SrVI, and 16SrXII were identified. Moreover, a number of fruits produced from the first-generation seedlings showed precocious seed germination, and the young seedlings resulted as phytoplasma-positive. The seed transmission of phytoplasmas in eggplants for two subsequent generations highlights the risk of additional sources of infection of the disease represented by asymptomatic and infected seedlings in the presence of insect vectors. The seed transmission could explain the continuous presence of epidemic outbreaks of phytoplasmas in brinjal cultivations in several cultivation areas.

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Section: Discussionmentioning

confidence: 99%

Eggplant Little Leaf-Associated Phytoplasma Detection in Seedlings under Insect-Proof Conditions

Darabakula,

Mateeti,

Pacini

et al. 2024

IJPB

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show abstract

“…In Telangana, Tamil Nadu and Tirupati, Andhra Pradesh, New Delhi, Uttar Pradesh, Maharashtra and Chhattisgarh, Kerala, Mysore and Dharwad, south Gujarat the phytoplasmas associated with brinjal little leaf all belonged to the 16SrVI group, mainly 16SrVI-D subgroup [30][31][32][33][34]. However, in Kadapa and Andhra Pradesh 16SrII-D phytoplasmas were also identified [35]. Kumar et al [18] reported little leaf disease of brinjal associated with 16SrI phytoplasmas at brinjal field of Bihar.…”

Section: Discussionmentioning

confidence: 99%

Brinjal Little Leaf Disease Epidemiology in Insect Proof Conditions

Darabakula,

Mateeti,

Pacini

et al. 2024

Preprint

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Eggplant or brinjal is one of the most consumed and important nutrient-dense tropical solanaceous vegetable crops grown worldwide. Little leaf is a disease especially widespread in brinjal in India where it is associated with the presence of phytoplasmas. To clarify the epidemiology of this disease and verify its seed transmission potential, seedlings and their progeny derived from symptomatic mother plants field-collected in Dharwad district of Karnataka State, India, were sowed under insect proof conditions in greenhouse. The resulting first- and second-generation seedlings obtained were tested at different times after germination by DNA extraction and amplification of 16S rRNA gene of phytoplasmas. Tissues from field collected symptomatic brinjal plants were also tested following the same procedure. The amplicons obtained were subjected to restriction fragment length polymorphism (RFLP) analysis and sequencing for phytoplasma identification. The detected phytoplasmas belonged to 16SrI, 16SrII, 16SrIII, 16SrV, 16SrVI, and 16SrXII groups. Several fruits produced from the first-generation seedlings showed germination of the seeds inside the fruit and resulted to be phytoplasma positive. This demonstrates the seed transmission of phytoplasmas in eggplants for two subsequent generations. This finding highlights the risk of additional source of infection represented by asymptomatic and infected seedlings under field conditions and contribute to explain the epidemic spread of phytoplasmas in brinjal cultivations in India and in the other eggplant growing areas of the world.

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Molecular identification of phytoplasmas associated with little leaf disease of brinjal in Andhra Pradesh and Telangana states of India

Cited by 2 publications

References 0 publications

Eggplant Little Leaf-Associated Phytoplasma Detection in Seedlings under Insect-Proof Conditions

Eggplant Little Leaf-Associated Phytoplasma Detection in Seedlings under Insect-Proof Conditions

Brinjal Little Leaf Disease Epidemiology in Insect Proof Conditions

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