2015
DOI: 10.7732/kjpr.2015.28.6.743
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Molecular Identification of Reynoutria japonica Houtt. and R. sachalinensis (F. Schmidt) Nakai Using SNP Sites

Abstract: -Reynoutria japonica and R. sachalinensis have been used as medicinal resources in Korea. However, it is difficult to identify and determine these medicinal herbs correctly because they are usually customized and purchased as the fragmented rhizomes types. To develop molecular markers for distinguishing two species, we analyzed and compared the chloroplast DNA sequences of seven loci (atpB, matK, ccD-psaI, atpF-H, trnL-trnF, psbK-I and rpl32-trnL). Among them, we found two effective SNPs in psbK-I region for R… Show more

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Cited by 2 publications
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“…There is even a report that seems to have treated R. japonica and R. forbesii as the same species [17]. Despite their outer morphological characteristics, and the fact that the genetic diversity or chemical constituents of some specimens have been previously reported, there are no previous reports of an anatomical or phytochemical study that can be applied to the decree of the botanical origin of the Reynoutria species [17][18][19][20][21][22][23][24][25]. Furthermore, it is very hard to discriminate crude drugs from these species precisely because they are usually sold as chopped rhizomes in the markets.…”
mentioning
confidence: 99%
“…There is even a report that seems to have treated R. japonica and R. forbesii as the same species [17]. Despite their outer morphological characteristics, and the fact that the genetic diversity or chemical constituents of some specimens have been previously reported, there are no previous reports of an anatomical or phytochemical study that can be applied to the decree of the botanical origin of the Reynoutria species [17][18][19][20][21][22][23][24][25]. Furthermore, it is very hard to discriminate crude drugs from these species precisely because they are usually sold as chopped rhizomes in the markets.…”
mentioning
confidence: 99%