Molecular identification of Sarcocystis wobeseri-like parasites in a new intermediate host species, the white-tailed sea eagle (Haliaeetus albicilla)

Shadbolt, Tammy; Pocknell, Ann; Sainsbury, A. W.; Egerton-Read, S.; Blake, Damer P.

doi:10.1007/s00436-021-07103-0

Cited by 11 publications

(22 citation statements)

References 13 publications

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“…The results of the present study extend the knowledge of S. halieti speci city for the IH and indicate that this species could form sarcocysts in the birds belonging to at least three different orders, Accipitriformes (present study), Charadriiformes [21] and Suliformes [20]. More avian Sarcocystis species, S. calchasi, S. columbae, S. falcatula, S. wobeseri can form sarcocysts in IH belonging to different orders [1,10,21,22]. The development of molecular research and expansion of the diversity of the examined host species leads to the detection of the known Sarcocystis species in different bird orders [22].…”

Section: Discussionsupporting

confidence: 77%

“…Subsequently, S. wobeseri was identi ed in the muscles of the white-tailed sea eagle from the UK [10].…”

Section: Discussionmentioning

confidence: 99%

“…Also, there are numerous studies about birds of prey as IH of Sarcocystis spp. [4][5][6][7][8][9][10]. It was recorded that pathogenic S. falcatula may cause encephalitis in the free-ranging great horned owl (Bubo virginianus) [6], the golden eagle (Aquila chrysaetos) and the bald eagle (Haliaeetus leucocephalus) [7].…”

Section: Introductionmentioning

confidence: 99%

“…Likewise, an undescribed Sarcocystis species causing encephalitis has been detected in an immature northern goshawk (Accipiter gentilis atricapillus) from Minnesota [4]. Recently, S. wobeseri was identi ed in pectoral and cardiac muscles of the white-tailed sea eagle (Haliaeetus albicilla) [10]. Thus, sarcocysts of two Sarcocystis species, S. falcatula and S. wobeseri, were recorded in the tissues of birds of prey [7,10].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, S. wobeseri was identi ed in pectoral and cardiac muscles of the white-tailed sea eagle (Haliaeetus albicilla) [10]. Thus, sarcocysts of two Sarcocystis species, S. falcatula and S. wobeseri, were recorded in the tissues of birds of prey [7,10]. Three morphological types of sarcocysts were detected in the Eurasian buzzard (Buteo buteo) and the long-eared owl (Asio otus), and the third type of sarcocyst distinguished in the owl was recognised as S. otus [11].…”

Section: Introductionmentioning

confidence: 99%

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Molecular Identification of Sarcocystis Halieti in Birds of Prey From Spain

Prakas

Bea²,

Juozaiė-Ngugu

et al. 2021

Preprint

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Background: Members of the genus Sarcocystis are protozoan parasites characterized by a prey-predator two-host life cycle. Sarcocysts are formed in muscles or CNS of the intermediate host (IH), while sporocysts develop in the small intestine of the definitive host (DH). Various birds of prey were confirmed to be DH for Sarcocystis spp. By contrast, only two species, S. wobeseri and S. falcatula were identified in the muscles of birds of prey. The latter species is pathogenic and can cause encephalitis in various birds. The aim of the present study was to identify Sarcocystis species in the muscles of birds of prey from Spain. Methods: In the period between 2019 and 2020, muscle tissues of 59 birds collected from Spain were examined for the presence of Sarcocystis spp. Sarcocysts in fresh squashed samples were morphologically characterised under a light microscope (LM). Sarcocystis species were identified by means of 28S rRNA and ITS1 sequence analysis. Results: With the help of methylene blue-staining microscopic sarcocysts were detected in 3/59 (5.1%) birds of prey from Spain. Under LM, one type of sarcocysts was observed. Sarcocysts were thread-like (1050–2160 × 130–158 μm), had a thin (0.7–1.4 μm) and smooth cyst wall. Septa divided the cysts into compartments filled with banana-shaped (5.9 × 1.7 μm) bradyzoites. On the basis of DNA sequence results, S. halieti was identified in the western marsh harrier (Circus aeruginosus) and the black kite (Milvus migrans) for the first time. Sarcocysts of S. halieti detected in the black kite and the western marsh harrier were shorter and wider as compared to those observed in the great cormorant (Phalacrocorax carbo) and the herring gull (Larus argentatus). Hence, S. halieti might infect birds belonging to three different orders, Suliformes, Charadriiformes and Accipitriformes. Conclusions: This is the first report of S. halieti in birds of prey as IH. Due to the inconsistency of research on Sarcocystis spp. from birds of prey, further complex morphological, histopathological, and molecular studies are required.

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Section: Discussionsupporting

confidence: 77%

“…Subsequently, S. wobeseri was identi ed in the muscles of the white-tailed sea eagle from the UK [10].…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular Identification of Sarcocystis Halieti in Birds of Prey From Spain

Prakas

Bea²,

Juozaiė-Ngugu

et al. 2021

Preprint

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First report of Sarcocystis falcatula in naturally infected Razorbill auks (Alca torda) collected in Tunisian Mediterranean Sea shores

Hammami,

Timoumi,

Larbi

et al. 2024

Parasitol Res

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First report of Sarcocystis halieti (Apicomplexa) in bearded vulture (Gypaetus barbatus)

et al. 2023

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At least three Sarcocystis species (S. falcatula, S. halieti and S. wobeseri–like) have been detected infecting raptorial birds. By histopathology and PCR-sequencing of the ITS1 marker, S. halieti was detected in a bearded vulture (Gypaetus barbatus) and a black kite (Milvus migrans) from the Catalonia region in North Spain. The 241 bp-long sequences obtained from the Sarcocystis organisms detected in both raptors showed 97.5–99.6% and 97.9–100% similarity with those of previously identified S. halieti; also, the phylogenetic trees generated placed the identified sequences together with other sequences of S. halieti available in GenBank. In sum, the description of the bearded vulture as a new intermediate host for S. halieti adds new insights on the complex epidemiology of the genus involving avian hosts.

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Molecular identification of Sarcocystis wobeseri-like parasites in a new intermediate host species, the white-tailed sea eagle (Haliaeetus albicilla)

Cited by 11 publications

References 13 publications

Molecular Identification of Sarcocystis Halieti in Birds of Prey From Spain

Molecular Identification of Sarcocystis Halieti in Birds of Prey From Spain

First report of Sarcocystis falcatula in naturally infected Razorbill auks (Alca torda) collected in Tunisian Mediterranean Sea shores

First report of Sarcocystis halieti (Apicomplexa) in bearded vulture (Gypaetus barbatus)

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