Molecular identification of similar species of the genus Biomphalaria (Mollusca: Planorbidae) determined by a polymerase chain reaction-restriction fragment length polymorphism

Analysis of the first and second internal transcribed spacer sequences of the ribosomal DNA in Biomphalaria tenagophila complex (Mollusca: Planorbidae)

“…Species abbreviations are as defined in Table. DISCUSSION In previous studies, using different molecular techniques, we reported a high degree of intra-specific variability in several species of Biomphalaria from different Brazilian localities, as well as from Uruguay and Argentina (Vidigal e al. 1994, 1996, 1998, Pires et al 1997, Caldeira et al 2001, Campos et al 2002. This variability is also observed when morphological characters such as vaginal pouch, vaginal wrinkling, penis sheath, prepuce, and prostatic diverticula are carefully analyzed (Paraense 1975(Paraense , 1981(Paraense , 1984(Paraense , 1888.…”

“…Paraense (1981) using the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) technique. This methodology has been successfully used in molecular systematic and also genetic variability studies among morphologically similar species (Vidigal et al 1998, Caldeira et al 1998). In fact, Spatz et al (1999), using PCR-RFLP and morphological analysis, proposed to cluster B. tenagophila, B. occidentalis, and B. t. guaibensis into the B. tenagophila complex based on their genetic and morphological similarities.…”

Morphological and polymerase chain reaction-restriction fragment lenght polymorphism characterization of Biomphalaria kuhniana and Biomphalaria amazonica from Colombia

“…Caldeira et al (1998), by using PCR-RFLP analysis of the ITS region of rRNA of B. straminea, B. intermedia, B. kuhniana and B. peregrina observed the cluster of three groups, which comprise: I) B. straminea and B. kuhniana; II) B. intermedia and III) B. peregrina. Groups I and II are more closely related while the third one showed to be a distant group.…”

A Multiplex-PCR approach to identification of the Brazilian intermediate hosts of Schistosoma mansoni

“…Paraense (1966) remarks that, due to the different environments occupied by some species, they show a high morphological intraspecific variation. Other species such as B. straminea, B. kuhniana and B. intermedia have very few morphological differences regarding the genital system, making their distinction difficult (Paraense 1988, Caldeira et al 1998.…”