Molecular identification of some Hoplolaimus species from the USA based on duplex PCR, multiplex PCR and PCR-RFLP analysis

Bae, Chang−Hwan; Robbins, R. T.; Szalanski, Allen L.

doi:10.1163/156854109x447042

Cited by 11 publications

(5 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The manufacturer's protocol was modified by reducing volumes to one eighth of the recommended amount (Ma et al, 2011). The ITS1 was amplified with primers Hoc-1f (5 -AACCTGCTGCTGGATCATTA-3 ) and LSUD3r (5 -TATGCTTAAGTTCAGCGGGT-3 ) following Bae et al (2008Bae et al ( , 2009; and a portion of cytochrome c oxidase subunit I (COI) sequence was amplified with primers JB3 (5 -TTTTTTGGGCATCCTGAGGTTTAT-3 ) and JB5 (5 -AGCACCTAAACTTAAAACATAATGAAA-3 ) (Derycke et al, 2005). For COI, the initial denaturation was set at 95°C for 3 min, followed by 33 cycles of 95°C for 45 s, 50°C for 1 min 15 s, 72°C for 2 min and final extension at 72°C for 10 min (Holguin et al, 2015a).…”

Section: Molecular Profiles and Phylogenymentioning

confidence: 99%

Morphological and molecular characterisation of Hoplolaimus smokyensis n. sp. (Nematoda: Hoplolaimidae), a lance nematode from Great Smoky Mountains National Park, USA

Robbins

Bernard

et al. 2019

Nematol.

View full text Add to dashboard Cite

SummaryHoplolaimus smokyensis n. sp. is a new species of lance nematode collected in Great Smoky Mountains National Park, USA. Females of H. smokyensis n. sp. have a labial region characterised by six, occasionally five, annules. The basal lip annule is subdivided by about 24 longitudinal striae. The stylet averages 47 μm long with robust, tulip-shaped stylet knobs bearing anterior projections. The hemizonid is ca 4 μm anterior to the excretory pore. The lateral field is incompletely areolated and has four continuous incisures from the metacorpus region to the tail region. There are three pharyngeal gland nuclei. Vulval epiptygma are absent. The scutellate phasmids are located one anterior and one posterior to the vulva. The male is shorter than the female and the head region is higher and more rounded than that of the female. The bursa extends to the tail tip and the gubernaculum is large and protrusible and has titillae and a capitulum. Morphologically, H. smokyensis n. sp. is most similar to H. galeatus and H. stephanus, but can be distinguished by differences such as the number of annules and longitudinal striae on the lip region and morphometric values. Hoplolaimus smokyensis n. sp. is also genetically distinct from other species according to comparisons of ribosomal and mitochondrial DNA sequences. Phylogenetic analyses based on ribosomal and mitochondrial gene sequences suggest that H. smokyensis n. sp. is a lineage distinct from related Hoplolaimus species.

show abstract

Section: Molecular Profiles and Phylogenymentioning

confidence: 99%

Morphological and molecular characterisation of Hoplolaimus smokyensis n. sp. (Nematoda: Hoplolaimidae), a lance nematode from Great Smoky Mountains National Park, USA

Robbins

Bernard

et al. 2019

Nematol.

View full text Add to dashboard Cite

show abstract

“…In addition, the PCR-RFLP method was applied by different researchers to evaluate the genetic diversity of Hoplolaimus spp. ( Robbins et al, 2009 ; Bae et al, 2009 ). Later, a species-specific primer was developed to distinguish Hoplolaimus stephanus Sher, 1963 from another similar species viz.…”

mentioning

confidence: 99%

Morphological and molecular characterization of Hoplolaimus pararobustus (Schuurmans Stekhoven and Teunissen, 1938) Sher 1963 with its first report on Zea mays roots in Namibia

Marais¹,

Berg²,

Fourie

et al. 2020

Journal of Nematology

View full text Add to dashboard Cite

In the summer of 2018, specimens of a Hoplolaimus population were extracted from a maize root sample collected near Stampriet, Namibia. This population was identified as Hoplolaimus pararobustus and is described and illustrated based on its morphological, morphometric, and molecular characteristics. To our knowledge, this is the first report H . pararobustus from maize roots. Females of the population had a mean body and stylet length of 1,100 µm and 36 µm, respectively. Esophagus with three nuclei in three pharyngeal glands. Lateral field reduced, ranging from a very faint line to just breaks in striae. The males were shorter than the females with a mean body length of 925 µm and the stylet slightly shorter, with a mean length of 34 µm. Phylogenetic analyses using partial sequences of 18 S and the expansion fragment D2–D3 of 28 S rDNA genes showed the close relation of this species and H. columbus . This Namibian population of H. pararobustus is the first Hoplolaimus species from Africa to be molecularly characterized.

show abstract

“…Sample collection: Twenty golf putting greens established with creeping bentgrass or a mix of creeping bentgrass and Poa annua were sampled in April, June, August and October of 2021 and 2022. Based on their broad geographic distribution and previously observed high lance nematode populations, seven putting green sites in Missouri and three in the Kansas City area of Kansas [KC: Mission Hills ( 2 ) and Olathe ( 1 )] were sampled in 2021. In 2022, ten additional sites in Indiana without historic nematode population data were sampled based on their broad geographic distribution across the state ( Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

“…The rDNA ITS region from morphologically identified lance nematodes was amplified with genus-level primer sequences Hoc-1f (5′- AACCTGCTGCTGGATCATTA-3′) and LSUD-03r (5′- TATGCTTAAGTTCAGCGGGT-3′) and were subsequently sequenced ( 1 ). The D2/D3 region of the 28S gene from morphologically identified root-knot nematodes was amplified with primer sequences ((RK28SF (5′- CGGATAGAGTCGGCGTATC-3′) and MR (5′- AACCGCTTCGGACTTCCACCAG-3′)) designed by Ye et al ( 36 ).…”

Section: Methodsmentioning

confidence: 99%

“…Amplicons were purified with ExoSAP-IT (ThermoFisher, Waltham, MA) following the manufacturer's recommendations and sent to Eurofins Genomics (Eurofins Genomics, Louisville, KY) for sequencing. Lance and root-knot sample DNA was also amplified using species-specific primers ( Table 3 ) developed for H. stephanus , H. columbus and H. galeatus ( 19 , 1 ) and M. naasi , M. marylandi , M. graminis and M. incognita ( 36 ), respectively. Species-specific amplicons were visualized after electrophoresis on a 1% agarose gel for further evidence of speciation.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Depth distribution of plant-parasitic nematodes on bentgrass golf greens in Missouri and Indiana

McCurdy,

Barizon,

Miller

2024

Journal of Nematology

View full text Add to dashboard Cite

Control of plant-parasitic nematodes (PPNs) on golf putting greens with nematicides is dependent on the seasonal occurrence and depth distribution of target PPN populations. This study aimed to determine if plant-parasitic nematode populations on golf course putting greens in Missouri and Indiana peaked at a targetable depth at a specific time in the year, focusing primarily on lance (Hoplolaimus spp.) and root-knot (Meloidogyne spp.) nematodes. To elucidate species diversity in the region, rDNA from a subset of lance and root-knot nematodes was sequenced and analyzed, with additional micromorphology of a lance nematode assessed in scanning electron micrographs (SEM). Soil samples were taken to a depth of 25 cm and stratified into 5 cm increments during April, June, August and October at seven sites across Missouri, three in the Kansas City metro of Kansas in 2021 and in ten sites across Indiana in 2022. Samples were stratified in five-centimeter increments and aggregated for a total of 100 cm3 of soil at each depth for each sampling. Samples were processed using a semi-automatic elutriator followed by the sucrose-flotation method, and populations were counted using a hemocytometer and recorded. For molecular characterization, rDNA was extracted and analyzed from 31 individual lance nematodes from one site in Missouri and eight sites in Indiana, and 13 root-knot nematodes from nine sites across Indiana. A significant interaction occurred between sampling month and depth for lance and ring nematodes Missouri/KS, with both PPN populations peaking at the 0–5 cm depth during October, which is well after most targeted nematicide applications are applied. Ring nematodes in Indiana did not follow this trend and were most abundant in August at a depth of 0–5 cm. No significant interaction between depth and month occurred for lance or root-knot nematodes in Indiana, or root-knot nematodes in Missouri/KS. Hoplolaimus stephanus and H. magnistylus were the lance species identified on golf greens, and Meloidogyne naasi, M. graminicola and M. marylandi were the root-knot species identified. Scanning-electron micrographs confirmed morphological characteristics unique to H. stephanus.

show abstract

Molecular identification of some Hoplolaimus species from the USA based on duplex PCR, multiplex PCR and PCR-RFLP analysis

Cited by 11 publications

References 10 publications

Morphological and molecular characterisation of Hoplolaimus smokyensis n. sp. (Nematoda: Hoplolaimidae), a lance nematode from Great Smoky Mountains National Park, USA

Morphological and molecular characterisation of Hoplolaimus smokyensis n. sp. (Nematoda: Hoplolaimidae), a lance nematode from Great Smoky Mountains National Park, USA

Morphological and molecular characterization of Hoplolaimus pararobustus (Schuurmans Stekhoven and Teunissen, 1938) Sher 1963 with its first report on Zea mays roots in Namibia

Depth distribution of plant-parasitic nematodes on bentgrass golf greens in Missouri and Indiana

Contact Info

Product

Resources

About