Molecular Identification of Staphylococcus aureus Isolates from Subclinical Mastitis in Cattle

Tresamol, P. V.; Suchithra, S.; Rathish, R. Joseph; Saseendranath, M. R.

doi:10.5455/ijlr.20170804045412

Cited by 1 publication

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“…bp confirming species identification (Fig.1.). Similar to our work,Yamagishi et al (2007),El- Razik, et al (2010) andTresamol et al (2018) used the same PCR technique for species level identification of S. aureus from bubaline and bovine milk respectively. In short, the prevalence of caprine mastitis was observed to be 15.15 per cent during a period of 8 months in the present study.A variable prevalence rate of S. aureus has been reported in lactating animals by different authors.…”

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confidence: 54%

“…The amplification procedure followed for pvl gene was an initial denaturation (95℃ for 5 min) followed by 30 cycles of denaturation (95℃ for 30 s), annealing (59℃ for 30s), extension (72℃ for 60s) and a final extension at 72℃ for 5 min). The DNA isolated from known S. aureus constituted the positive control (Tresamol et al, 2018) and DNA from known Escherichia coli constituted the negative control (confirmed at Department of Veterinary Epidemiology and Preventive Medicine, College of Veterinary and Animal Sciences, Mannuthy). The PCR products were subjected to 1.5 per cent agarose gel electrophoresis for visualisation and documentation of the amplified products.…”

Section: Methodsmentioning

confidence: 99%

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Molecular detection of methicillin resistant Staphylococcus aureus associated with mastitis in goats

Bhagya¹,

Tresamol²,

Vijayakumar³

et al. 2022

Journal of Veterinary and Animal Sciences

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Antimicrobial resistance is an emerging concern in the world while combating with a wide range of pathogen associated with various disease conditions. It is considered as a silent pandemic nowadays. A constant monitoring and measures for controlling emergence of antimicrobial resistance are crucial in the current situation. The present study was conducted with the objective of detection of methicillin resistant Staphylococcus aureus (MRSA) associated with mastitis in goats. Milk samples were collected from 66 goats suffering from mastitis and screened for the presence of S. aureus. Conventional microbiological tests and species specific PCR were employed for the species confirmation. A total of 10 samples (15.15%) were found to be positive for S. aurues. Among the ten isolates of S. aureus obtained two isolates (3.03%) were found to harbour mecA gene suggestive of the presence of MRSA phenotype. All the isolates were negative for the presence of pvl gene which is present in highly virulent strains. In short, even though the study demonstrated the presence of MRSA at a lower rate, the results are of a marked public health significance due to the zoonotic significance of the pathogen.

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confidence: 54%

Section: Methodsmentioning

confidence: 99%