Molecular identification of the agent of Q fever – Coxiella burnetii – in domestic animals in State of Rio de Janeiro, Brazil

Mares-Guia, Maria Angélica Monteiro de Mello; Rozental, Tatiana; Guterres, Alexandro; Gomes, Raphael; Almeida, D.; Moreira, Namir Santos; Barreira, Jairo Dias; Favacho, Alexsandra Rodrigues de Mendonça; Santana, Andrea Lopes; Lemos, Elba Regina Sampaio de

doi:10.1590/0037-8682-0076-2013

Cited by 42 publications

(36 citation statements)

References 10 publications

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“…Since the 1950s, several studies have demonstrated that wind plays a role in C. burnetii transmission. [28][29][30] In this context, our results, in combination with conclusions from these studies associated with the presence of infected animals (sheep, dogs, and cats) in the index case's house, 11,12 indicate that wind can be an epidemiologic factor in Q fever outbreaks occurring near sheep-rearing areas.…”

Section: Discussionsupporting

confidence: 51%

“…Cases 1, 2, 6, 7, and 8 were identified in peri-urban areas within 5 km of index area where animals that excreted C. burnetii were also detected. 12 The other four cases were located more than 10 km away from the index case's house, in farm areas. One of these nine patients also had dengue infection, indicating that the patient was coinfected with both pathogens.…”

Section: Resultsmentioning

confidence: 99%

“…This hospital-based crosssectional study was conducted at the Desembargador Leal Junior Hospital (DLJH) in the municipality of Itaboraí (22°44′51′′ S, 42°51′21′′ W), Rio de Janeiro, where C. burnetii infection was detected in human and domesticated animals in 2008. 11,12 From March 2013 to October 2014, all patients admitted to DLJH with a diagnosis of dengue infection and who lived in Itaboraí or the surrounding areas were considered for entry into the study. Clinical and epidemiological data were systematically collected by the hospital's surveillance group using the national dengue surveillance system (Information System for Notifiable Diseases).…”

Section: Methodsmentioning

confidence: 99%

“…9 Although serological tests for C. burnetii infection have been available in Brazil since 1953, 10 it was not until 2008 that the bacterium was characterized molecularly from a patient with fever, thrombocytopenia, and a history of contact with goat abortion products in Rio de Janeiro, Brazil. 11,12 Since then, although sporadic cases have been confirmed [13][14][15] including endocarditis, the most severe form of chronic disease, 16 it was only in 2014 that Q fever become a notifiable disease in Brazil. Acute Q fever is difficult to diagnose because it shares similar manifestations with a large number of infectious diseases, for example, fever, headache, myalgia, and/or pneumonia.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Identification of Q Fever in Patients with a Suspected Diagnosis of Dengue in Brazil in 2013–2014

Mares-Guia¹,

Rozental²,

Guterres³

et al. 2016

The American Society of Tropical Medicine and Hygiene

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Abstract. Q fever is an important cause of undifferentiated fever that is rarely recognized or reported in Brazil. The objective of this study was to look for the presence of Coxiella burnetii during a dengue fever outbreak in the municipality of Itaboraí, Rio de Janeiro, Brazil, where this bacterium had previously infected humans and domesticated animals. Blood samples from clinically suspected dengue fever patients were tested by polymerase chain reaction (PCR) for C. burnetii; the DNA was detected in nine (3.3%) of 272 patients. One was coinfected with dengue virus, which was also detected in another 166 (61.3%) patients. The nucleotide sequence of PCR amplification and DNA sequencing of the IS1111 transposase elements in the genome of C. burnetii exhibited 99% identity with the sequence in GenBank. The detection of C. burnetii in patients suspected of dengue fever indicates that awareness and knowledge of Q fever should be strengthened and that this bacterium is present in Brazil. Finally, because a negative molecular result does not completely rule out the diagnosis of Q fever and the serological assay based on seroconversion was not available, the actual number of this zoonosis is likely to be much higher than that reported in this study.

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Section: Discussionsupporting

confidence: 51%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular Identification of Q Fever in Patients with a Suspected Diagnosis of Dengue in Brazil in 2013–2014

Mares-Guia¹,

Rozental²,

Guterres³

et al. 2016

The American Society of Tropical Medicine and Hygiene

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“…In Hungary, 1 sample in a total of 126 shepherd, hunting, and stray dogs was PCR-positive, while 20.3% of dogs seroconverted [20]. Based on molecular results, this pathogen was also identified in dogs from Japan [127] and Brazil [128].…”

Section: The Disease In Dogsmentioning

confidence: 97%

Bacterial canine vector-borne zoonotic diseases in “One Health” concept

Valiakos

2016

Int J One Health

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Canine vector-borne diseases constitute a large group of diseases transmitted by arthropods with worldwide distribution. A wide range of bacterial, viral, and parasitic agents that are transmitted by vectors cause disease to dogs, many of which can also affect humans and thus have an important zoonotic potential. Bacterial agents that are transmitted by vectors have been considered less important than viral or parasitic agents and are not commonly discussed in companion animal practice. However, close contact between pet animals and people offers favorable conditions for transmission of these bacteria. Many of these diseases have become a focus of interest for scientists in recent years. Increase in reservoir abundance, climate change, changing habitat structure, socio-political changes, and imports of dogs for welfare reasons and trade as well as traveling are considered to be potential factors for the pathogens and vectors introduction into new areas. Apart from, the veterinary aspect of these diseases, domestic dogs could play a central epidemiological role in the transmission of bacterial agents to humans, acting as reservoirs and sentinels, a circumstance that requires a One Health approach. This review highlights the most important of these bacterial agents, presenting updated current knowledge with special reference to treatment approach and One Health aspect.

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A rapid bio‐optical sensor for diagnosing Q fever in clinical specimens

Koo

Jin

Park

et al. 2018

Journal of Biophotonics

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Recent zoonotic outbreaks, such as Zika, Middle East respiratory syndrome and Ebola, have highlighted the need for rapid and accurate diagnostic assays that can be used to aid pathogen control. Q fever is a zoonotic disease caused by the transmission of Coxiella burnetii that can cause serious illness in humans through aerosols and is considered a potential bioterrorism agent. However, the existing assays are not suitable for the detection of this pathogen due to its low levels in real samples. We here describe a rapid bio-optical sensor for the accurate detection of Q fever and validate its clinical utility. By combining a bio-optical sensor, that transduces the presence of the target DNA based on binding-induced changes in the refractive index on the waveguide surface in a label-free and real-time manner, with isothermal DNA amplification, this new diagnostic tool offers a rapid (<20 min), 1-step DNA amplification/detection method. We confirmed the clinical sensitivity (>90%) of the bio-optical sensor by detecting C. burnetii in 11 formalin-fixed, paraffin-embedded liver biopsy samples from acute Q fever hepatitis patients and in 16 blood plasma samples from patients in which Q fever is the cause of fever of unknown origin.

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Molecular identification of the agent of Q fever – Coxiella burnetii – in domestic animals in State of Rio de Janeiro, Brazil

Cited by 42 publications

References 10 publications

Molecular Identification of Q Fever in Patients with a Suspected Diagnosis of Dengue in Brazil in 2013–2014

Molecular Identification of Q Fever in Patients with a Suspected Diagnosis of Dengue in Brazil in 2013–2014

Bacterial canine vector-borne zoonotic diseases in “One Health” concept

A rapid bio‐optical sensor for diagnosing Q fever in clinical specimens

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