Molecular identification of the Asian lady beetle Harmonia axyridis in Israel

Pines, Gur; Partosh, Tamir; Protasov, Alex; Kramer, Reut; Singh, Shashwat; Davidovitz, Michael; Mazor, Michal; Kaspi, Roy

doi:10.3391/bir.2022.11.4.07

Cited by 3 publications

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“…As such, H. axyridis was extensively used for biocontrol purposes across the globe and is still being used in several locations. However, H. axyridis also displays high invasiveness properties, spreading beyond the original release sites (Kenis et al, 2020;Koch, 2003;Lombaert et al, 2010;Pines et al, 2022). H. axyridis has four primary phenotypic forms; three include a black background with red or orange spots (Forma conspicua, F. spectabilis, and F. axyridis), whereas the fourth form (F. succinea) displays black spots over a red background (Michie et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

CRISPR-Based Genome Editing inHarmonia Axyridis

Partosh,

Davidovitz,

Firer

et al. 2023

Preprint

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Harmonia axyridis(Pallas), commonly known as the Asian lady beetle, is a native insect species of Asia that has been intentionally introduced to various regions for biocontrol purposes. However, its widespread presence beyond its original release sites suggests a high degree of invasiveness. Previous studies have employed double-stranded RNA techniques to investigate the functional genomics of this species. In this study, we utilized the CRISPR-Cas9 approach to achieve precise genome editing inH. axyridis. Specifically, we targeted two distinct genes known to exhibit visible phenotypic effects. While the knockout of thelaccase2gene resulted in an early-detectable phenotype but also in lethality, we successfully established a viable and genetically stable mutant colony by disrupting thescarletgene. Our findings contribute to the expanding knowledge of genetic manipulation inH. axyridisand provide insights into its potential for future research and practical applications for biocontrol and invasive species management.

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Section: Introductionmentioning

confidence: 99%

CRISPR-Based Genome Editing inHarmonia Axyridis

Partosh,

Davidovitz,

Firer

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

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Ladybirds: biocontrol agents

Shandilya,

Singh,

Mishra

et al. 2024

Biocontrol Agents for Improved Agriculture

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Stable clustered regularly interspaced short palindromic repeats‐based genome editing in Harmonia axyridis

Partosh,

Davidovitz,

Firer

et al. 2024

J Applied Entomology

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Harmonia axyridis (Pallas), commonly known as the Asian lady beetle, is a native insect species of Asia that has been intentionally introduced to various regions for biocontrol purposes. However, its widespread presence beyond its original release sites suggests a high degree of invasiveness. In this study, we utilized the CRISPR‐Cas9 approach to achieve precise genome editing in H. axyridis. Specifically, we targeted two genes in H. axyridis, laccase2 and scarlet, knockdown of which orthologues in other insects showed visible phenotypic changes. The knockout laccase2 resulted not only in an early‐detectable phenotype but also in lethality. However, we successfully established a viable and genetically stable mutant colony by disrupting the scarlet gene, resulting in beetles with white eyes. Our findings contribute to the expanding knowledge of genetic manipulation in H. axyridis and provide insights into its potential for future research and practical applications for biocontrol and invasive species management.

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Molecular identification of the Asian lady beetle Harmonia axyridis in Israel

Cited by 3 publications

References 0 publications

CRISPR-Based Genome Editing inHarmonia Axyridis

CRISPR-Based Genome Editing inHarmonia Axyridis

Ladybirds: biocontrol agents

Stable clustered regularly interspaced short palindromic repeats‐based genome editing in Harmonia axyridis

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