Molecular Identification of the Colony-Associated Cultivable Bacteria of the Cyanobacterium<i>Microcystis aeruginosa</i>and Their Effects on Algal Growth

Shi, Limei; Cai, Yuanfeng; Li, Pengfu; Yang, Haiwang; Liu, Zhili; Kong, Ling-Dong; Yang, Yu; Kong, Fanxiang

doi:10.1080/02705060.2009.9664285

Cited by 24 publications

(19 citation statements)

References 37 publications

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“…Especially for bacterium L4, it shows high similarity to Pseudomonas sp. A8, which was reported to be also found in the cyanobacterial cultures [22]. Furthermore, these bacteria considerably enhanced H 2 accumulation of Cr849 ( Figs.…”

Section: Discussionsupporting

confidence: 68%

Improvement of hydrogen production of Chlamydomonas reinhardtii by co-cultivation with isolated bacteria

Huang

et al. 2013

International Journal of Hydrogen Energy

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Co-culture Isolated bacteriaHydrogen production a b s t r a c t Three bacteria, named L2, L3 and L4, were isolated from contaminated cultures of Chlamydomonas reinhardtii strain cc849 in laboratory. The phylogenetic analysis based on 16S rDNA sequences showed that L2, L3 and L4 belonged to genus Stenotrophomonas, Microbacterium and Pseudomonas, respectively. The co-cultivation of isolated L2, L3 and L4 with purified algae, respectively, demonstrated that moderate bacterial concentration did not affect algal growth significantly but improved algal H 2 production obviously. The maximal H 2 yields were gained by the co-culture of algae with L2 or L4, about 4.0 times higher than that of the single algal culture. Increased respiration rate or O 2 consumption was the main reason for the enhancement of H 2 yield of the co-cultures. journal h om epa ge: www.elsev ier.com/locate/he i n t e r n a t i o n a l j o u r n a l o f h y d r o g e n e n e r g y 3 8 ( 2 0 1 3 ) 1 0 7 7 9 e1 0 7 8 7

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Section: Discussionsupporting

confidence: 68%

Improvement of hydrogen production of Chlamydomonas reinhardtii by co-cultivation with isolated bacteria

Huang

et al. 2013

International Journal of Hydrogen Energy

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“…In the global alphaproteobacterial phylogeny, this clade and several other lineages that include exclusively environmental clones (with the one exception of Woodsholea maritima ) form a sister lineage to the Hyphomonadaceae clade, although the bootstrap statistical supports were weak (<50%, both in NJ and ML). According to the study that detected the A4 and A10 sequences, they both originated from a colony of the cyanobacterium Microcystis aeruginosa 34 . This organism is one of the main constituents of cyanobacterial water blooms that occur in eutrophic freshwater environments worldwide 35 .…”

Section: Resultsmentioning

confidence: 99%

A novel alphaproteobacterial ectosymbiont promotes the growth of the hydrocarbon-rich green alga Botryococcus braunii

Tanabe

Okazaki

Yoshida

et al. 2015

Sci Rep

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Botryococcus braunii is a colony-forming green alga that accumulates large amounts of liquid hydrocarbons within the colony. The utilization of B. braunii for biofuel production is however hindered by its low biomass productivity. Here we describe a novel bacterial ectosymbiont (BOTRYCO-2) that confers higher biomass productivity to B. braunii. 16S rDNA analysis indicated that the sequence of BOTRYCO-2 shows low similarity (<90%) to cultured bacterial species and located BOTRYCO-2 within a phylogenetic lineage consisting of uncultured alphaproteobacterial clones. Fluorescence in situ hybridization (FISH) studies and transmission electric microscopy indicated that BOTRYCO-2 is closely associated with B. braunii colonies. Interestingly, FISH analysis of a water bloom sample also found BOTRYCO-2 bacteria in close association with cyanobacterium Microcystis aeruginosa colonies, suggesting that BOTRYCO-2 relatives have high affinity to phytoplankton colonies. A PCR survey of algal bloom samples revealed that the BOTRYCO-2 lineage is commonly found in Microcystis associated blooms. Growth experiments indicated that B. braunii Ba10 can grow faster and has a higher biomass (1.8-fold) and hydrocarbon (1.5-fold) yield in the presence of BOTRYCO-2. Additionally, BOTRYCO-2 conferred a higher biomass yield to BOT-22, one of the fastest growing strains of B. braunii. We propose the species name ‘Candidatus Phycosocius bacilliformis’ for BOTRYCO-2.

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“…Since it is able to release amino acids and solubilize phosphates, Stenotrophomonas may form a mutualistic relationship with its host (Shi et al. ). This relationship could correspond to their positive associations with some microeukaryotes (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Stenotrophomonas was found as an endocytic bacterium in Noctiluca scintillans and reported as being associated with the nutrition of cyanobacteria (Seibold et al 2001). Since it is able to release amino acids and solubilize phosphates, Stenotrophomonas may form a mutualistic relationship with its host (Shi et al 2009). This relationship could correspond to their positive associations with some microeukaryotes ( Fig.…”

Section: T-rfsmentioning

confidence: 99%

An association network analysis among microeukaryotes and bacterioplankton reveals algal bloom dynamics

Tan

Zhou

Zhu

et al. 2015

Journal of Phycology

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Algal blooms are a worldwide phenomenon and the biological interactions that underlie their regulation are only just beginning to be understood. It is established that algal microorganisms associate with many other ubiquitous, oceanic organisms, but the interactions that lead to the dynamics of bloom formation are currently unknown. To address this gap, we used network approaches to investigate the association patterns among microeukaryotes and bacterioplankton in response to a natural Scrippsiella trochoidea bloom. This is the first study to apply network approaches to bloom dynamics. To this end, terminal restriction fragment (T-RF) length polymorphism analysis showed dramatic changes in community compositions of microeukaryotes and bacterioplankton over the blooming period. A variance ratio test revealed significant positive overall associations both within and between microeukaryotic and bacterioplankton communities. An association network generated from significant correlations between T-RFs revealed that S. trochoidea had few connections to other microeukaryotes and bacterioplankton and was placed on the edge. This lack of connectivity allowed for the S. trochoidea sub-network to break off from the overall network. These results allowed us to propose a conceptual model for explaining how changes in microbial associations regulate the dynamics of an algal bloom. In addition, key T-RFs were screened by principal components analysis, correlation coefficients, and network analysis. Dominant T-RFs were then identified through 18S and 16S rRNA gene clone libraries. Results showed that microeukaryotes clustered predominantly with Dinophyceae and Perkinsea while the majority of bacterioplankton identified were Alphaproteobacteria, Gammaproteobacteria, and Bacteroidetes. The ecologi-cal roles of both were discussed in the context of these findings.

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Molecular Identification of the Colony-Associated Cultivable Bacteria of the CyanobacteriumMicrocystis aeruginosaand Their Effects on Algal Growth

Abstract: Fourteen bacteria were isolated from a natural bloom of Microcystis aeruginosa colonies collected from Lake Taihu. According to 16s rDNA sequence analysis, seven isolates were related to Alphaproteobacteria including the genera Erythromicrobium,

Cited by 24 publications

References 37 publications

Improvement of hydrogen production of Chlamydomonas reinhardtii by co-cultivation with isolated bacteria

Improvement of hydrogen production of Chlamydomonas reinhardtii by co-cultivation with isolated bacteria

A novel alphaproteobacterial ectosymbiont promotes the growth of the hydrocarbon-rich green alga Botryococcus braunii

An association network analysis among microeukaryotes and bacterioplankton reveals algal bloom dynamics

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