Molecular identification of the rumen flukes Paramphistomum leydeni and Paramphistomum cervi in a concurrent infection of the red deer Cervus elaphus

Sindičić, Magda; Martinković, Franjo; Strišković, Tanja; Špehar, Marina; Štimac, Iva; Bujanić, Miljenko; Konjević, Dean

doi:10.1017/s0022149x16000699

Cited by 12 publications

(6 citation statements)

References 18 publications

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“…The BLAST analysis of the obtained sequence revealed 100% similarity with P. cervi of China and 98% with P. epiclitum and other Paramphistomum species of India indicating the common phylogenetic history owing to the results of a previous study [25,29]. This similarity may be due to the fact that these areas are neighboring to Pakistan and sharing the same geographical, environmental conditions with free movement of infected hosts.…”

Section: Discussionmentioning

confidence: 67%

Molecular Characterization of Paramphistomum cervi in Buffaloes

Rafiq

Niaz

Zeb

et al. 2020

Acta Scientiae. Vet.

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Background: Paramphistomiasis (Rumen fluke disease) in ruminants is a major health problem, while documented reports on Paramphistomum species are limited in Asian countries. The present study aimed to identify paramphistomoid flukes that infects buffaloes with the goal of characterization of prevalence in Pakistan and its comparison with neighbor countries.Materials, Methods & Results: In 2018, a total of 178 slaughtered buffaloes aged four to six years were examined and flukes were collected from their infected rumen and reticulum using sterilized forceps. After amplification and sequencing of 18S rRNA partial fragment, the generated sequences were edited (810bp) and aligned with the other sequences of Paramphistomum species retrieved from NCBI. A phylogenetic tree was constructed using maximum likelihood method in MEGA X. The 18S rRNA sequence was found 100% similar with Paramphistomum cervi of China and 98% with Paramphistomum epiclitum and other Paramphistomum species of India. The parasitic Pharamphistomum species was identified molecularly as P. cervi.Discussion: Molecular studies provide insight into the biology and phylogenetic relationship among various parasites. These studies are reliable in the genetic-based identification and description of several disease causing agents. The 18S rRNA sequence of P. cervi generated in this study was found closely identical to the P. cervi of the neighbor countries (China and India) which may be due to the similar geographical, environmental conditions and transboundary movement of infected hosts. This is the first nature of study which provides the molecular-based evidence of P. cervi existence in Pakistan and revealed the 18S rRNA as novel molecular marker for the identification and further characterization of Paramphistomum species across Pakistan. The submitted sequence of this study will provide a baseline for further molecular characterization and to compare with other Paramphistoma species from different regions of Pakistan.

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Section: Discussionmentioning

confidence: 67%

Molecular Characterization of Paramphistomum cervi in Buffaloes

Rafiq

Niaz

Zeb

et al. 2020

Acta Scientiae. Vet.

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“…In the past, the histomorphological examination of fluke specimens of bovine origin from Germany led to a controversial interpretation with respect to the identification of P. leydeni , which was considered synonymous with P. cervi by some authors (Odening et al 1978 ; Eduardo 1982 ; Odening 1983 ). Using molecular diagnosis, P. leydeni has been identified parasitizing cervids in Europe in the recent past, including one case of co-infection with P. cervi (O’Toole et al 2014 ; Sindičić et al 2017 ), but was occasionally identified from fluke eggs excreted by sheep and cattle in Ireland and the Netherlands, respectively (Martinez-Ibeas et al 2016 ; Ploeger et al 2017 ).…”

Section: Resultsmentioning

confidence: 99%

DNA barcoding of rumen flukes (Paramphistomidae) from bovines in Germany and Austria

et al. 2021

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Rumen flukes have received growing veterinary attention in western and central Europe during the past two decades because of an increase in prevalence of infection in cattle and sheep, including cases of severe clinical disease. Historically, rumen fluke infections in Europe were assumed to be caused mainly by Paramphistomum cervi (or species, which were later considered to be synonymous with P. cervi), but more recently molecular studies demonstrated Calicophoron daubneyi to be the predominating species. For the present investigation, adult rumen flukes isolated from 23 cattle originating from ten farms in Germany (Saxony [1], Baden-Württemberg [4], Bavaria [5]) and one farm in Austria (Tyrol) were analyzed to establish partial sequences of the mitochondrial cytochrome c oxidase subunit I (COI) and the complete sequence of the nuclear internal transcribed spacer 2 (ITS2). Flukes of five animals (dairy cows from three farms in Bavaria) were determined as P. leydeni, and flukes of 18 animals (dairy cows or cattle from cow-calf operations from eight farms in Saxony [1], Baden-Württemberg [4], Bavaria [2], and Tyrol [1]) were identified as C. daubneyi. Based on the molecular analysis of adult rumen flukes collected from cattle, the results of this investigation confirm the common occurrence of C. daubneyi in Germany and reveal the first definitive findings of P. leydeni in Germany and C. daubneyi in Austria.

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“…Paramhistomum parasites collected from a reindeer in Croatia reported P.cervi and P. leydeni as a result of sequence analysis conducted in the rDNA ITS-2 gene region by molecular methods (23). Sanabria et al (19) Paramphistomum leydeni was identified molecular using 5.8S and 28S partial ITS-2 rDNA sequences.…”

Section: Discussionmentioning

confidence: 99%

Molecular identification of Paramphistomidae obtained from ruminants in Van province

Padak¹,

Karakuş

2021

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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This study was aimed to identify Paramphistomum species from infected ruminants (sheep and cattle) by molecular methods between March 2018 and September 2018 at Van municipality slaughterhouse. In the research, the rDNA ITS-2 gene region of adult Paramphistomums was amplified by PCR method. Amplicons 399 bp long were viewed in agarose gel. As a result of bidirectional sequence analysis made from PCR amplicons, Paramphistomum leydeni and Calicophoron daubneyi species were identifyed. Amplicon sequences were compared by BLAST with reference sequences from Genbank. Phylogenetic tree was created with the Neighbor-Joining method by using the MEGA 7 program. Genotypes obtained from isolates were compared for exact or closest similarities. In conclusion, C. daubneyi, which was previously detected by morphological methods in Turkey, in this study, it was identified for the first time by using molecular methods. Also in this study, P. leydeni was reported for the first time in Turkey.

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Molecular identification of the rumen flukes Paramphistomum leydeni and Paramphistomum cervi in a concurrent infection of the red deer Cervus elaphus

Cited by 12 publications

References 18 publications

Molecular Characterization of Paramphistomum cervi in Buffaloes

Molecular Characterization of Paramphistomum cervi in Buffaloes

DNA barcoding of rumen flukes (Paramphistomidae) from bovines in Germany and Austria

Molecular identification of Paramphistomidae obtained from ruminants in Van province

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