Molecular identification of virulence genes of Pseudomonas aeruginosa isolated from fish (Cyprinus carpio) in Mosul city

Altaee, Asmaa J.; Aldabbagh, Sumaya Y.

doi:10.33899/ijvs.2022.132660.2119

Cited by 4 publications

(5 citation statements)

References 15 publications

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“…The microbial load was highest (gills and fins) and may be attributed to the highest ambient river temperature. Microbial load in fish may rise with increasing temperature of water [16,17].In study [18] acquired eighty isolates of P. aeruginosa from samples of fish market specimens' gills, skin, gut, and muscles. The gills had the largest proportion of isolation at 42.5%, followed by the skin at 37.5%, the intestine at 15%, and the muscles at 5%.…”

Section: Discussionmentioning

confidence: 99%

“…The maximum sensitivity of drug screening in different organisms is a reasonable technique that can significantly reduce the dose of antibiotics. After starting therapy, isolates that were initially susceptible or intermediately resistant to one antibiotic may develop resistance [18].Ipenem and gentamicin were both ineffective against 96% of the isolates, whereas doxycycline, ciprofloxacin, trimethoprim-sulfamethoxazole, norfloxacin, and ceftriaxone were next in line. None of them were levofloxacin-resistant on Disease Aeromonas hydrophila [21].…”

Section: Discussionmentioning

confidence: 99%

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A First Record of Sphingobacterium thalpophilum Isolated from Coptodon zillii and Oreeochromis aureus in the Al-Diwaniya River/Iraq

Al-Jubouri,

Obead,

Salman

2023

IOP Conf. Ser.: Earth Environ. Sci.

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This investigation was done to determine whether C. zillii and O. aureus in the Al-Diwaniya Stream included every possibly harmful species of Gram-negative microbes. 100 C. zillii and 100 O. aureus fish samples were taken from the Al-Diwaniya River between Apr 2021 and Mar 2022. bacteria he was found us the VITEK 2 system. Here, we reported the discovery of S. thalpophilum, which was isolated from the intestine, fins, and gills of C. zillii and O. aureus, for the first time in Iraq. S. thalpophilum were found in C. zillii with a different proportion as follows: skin 1%, gills 70%, fins 24%, and intestine 5%; and in O. aureus with a different percentage as follows: skin 1%, gills 49%, fins 30%, and intestine 20%. Additionally, the VITEK 2 system was utilized to antibiotic sensitivity. The findings showed that the bacteria were susceptible to minimum inhibitory concentration (MIC) of imipenem, ciprofloxacin, and levofloxacin (0.25 g/ml), as well as to S. thalpophilum, but resistant to cefazolin (>= 61 g/ml).

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A First Record of Sphingobacterium thalpophilum Isolated from Coptodon zillii and Oreeochromis aureus in the Al-Diwaniya River/Iraq

Al-Jubouri,

Obead,

Salman

2023

IOP Conf. Ser.: Earth Environ. Sci.

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“…(30). A study reported that the lethal concentrations of CuSO4 at 10 mg/l led to killing the fish, and mortality was 100% during 24 hours, while the concentration at five mg/l was the median lethal concentration which led to killing 50% of treated fish (31,32).…”

Section: Discussionmentioning

confidence: 99%

Bioaccumulation of heavy metals in common carp fish (Cyprinus carpio) and its relationship with the protein content

Kareem¹,

Husain²,

Rasheed³

2022

IJVS

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The objective of the present study was to investigate the concentration of some heavy metals in the dorsal muscle, gills, and liver of common carp (Cyprinus carpio) from the Dukan lake, Suleimani, Iraq, and its relationship with fish protein content. The sampling was carried out in both the summer and winter seasons of 2021. Fish samples were collected from the water of Dukan lake. Biometry analysis and determining of protein content were carried out. Moreover, the heavy metal concentrations were measured in different tissues and organs of fish (the dorsal muscle, gills, and liver). The metal concentration varied considerably among different tissues and organs. Overall, the mean metal concentration revealed an order of Zn > Fe > Cr > Cd > Cu > As > Pb. The mean concentrations of Zn and Pb were determined as the maximum and minimum concentrations of metals in the fish organs, respectively. Metal accumulation was higher in the gills and liver than in the muscle (except Cr). The content of protein showed a close relationship with some of the metal concentrations. The concentrations of metals like As, Cd, and Cu were higher during the summer than in the winter, except for Cr and Fe. Due to the concerns related to the adverse effects of heavy metals in fish meat on human health, investigation of the concentration of pollution and regular monitoring of the physicochemical and heavy metal contents and its relationship with those measured in fish is strongly recommended.

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“…The electrophoresis condition was set at 80 V, 300 mA ,50 min by using Wide Mini-Sub Cell GT gel electrophoresis systems and basic power supply (Bio-Rad, USA), A 4 µl of 100 bp DNA ladder, (GeNet Bio, Korea) was used as molecular weight standard. After that, the gel was viewed using Gel doc Ez system to detect the specific bands (18,19).…”

Section: Detection Of E Coli and Their Esbl Gene By Pcr Reaction Methodsmentioning

confidence: 99%

Detection of extended spectrum beta lactam producing Escherichia coli isolated from Cyprinus carpio in Mosul city

Mahmmoud¹,

Aldabbagh²

2022

IJVS

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Extended-spectrum b-lactamase (ESBL) producing Escherichia coli constitute an emerging health problem globally, fish act as a potential reservoir for ESBLs E. coli and serve as a vehicle of transmission of ESBL resist genes to others strains of bacteria. A total of 75 samples of Cyprinus carpio were collected from the local fish market of Mosul city during the period between) October 2021 to February 2022(. each sample was placed separately in a sterile plastic bag and transported directly under cooling conditions to the microbiology lab, College of Veterinary Medicine. ESBLs producing Escherichia coli were isolated and characterized using MacConkey agar medium supplemented with 2 mcg/ml cefotaxime. Twenty-six isolates 34% of fish gut samples were obtained. A polymerase chain reaction was carried out to confirm the results of the isolation using special primers for E. coli (ECO223-f, ECO223-r). Resistance genes assay were performed using the primers Cefotaxamase (CTX-M) and sulphydryl variable (SHV). All isolates showed that possessed the CTX-M gene 100%, while none of the isolates possessed the SHV gene. This study showed that fish play a major role in the transmission of broad-spectrum beta-lactam-resistant Escherichia coli to humans as a result of handling or marketing it, or by consuming contaminated or infected fish.

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Molecular identification of virulence genes of Pseudomonas aeruginosa isolated from fish (Cyprinus carpio) in Mosul city

Cited by 4 publications

References 15 publications

A First Record of Sphingobacterium thalpophilum Isolated from Coptodon zillii and Oreeochromis aureus in the Al-Diwaniya River/Iraq

A First Record of Sphingobacterium thalpophilum Isolated from Coptodon zillii and Oreeochromis aureus in the Al-Diwaniya River/Iraq

Bioaccumulation of heavy metals in common carp fish (Cyprinus carpio) and its relationship with the protein content

Detection of extended spectrum beta lactam producing Escherichia coli isolated from Cyprinus carpio in Mosul city

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