Molecular identification targeting cox1 and 18S genes confirms the high prevalence of Sarcocystis spp. in cattle in the Netherlands

Hoeve-Bakker, B. J. A.; Giessen, J.W.B. van der; Franssen, Frits

doi:10.1016/j.ijpara.2019.05.008

Cited by 28 publications

(29 citation statements)

References 33 publications

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“…For example, S. heydorni tissue tropism was noted in cattle from China [35], with the highest prevalence (9.7%) recorded in skeletal muscles, followed by 3.4% prevalence in the oesophagus, 2.5% in the diaphragm and 0.1% in the tongue muscles; however, S. heydorni was absent in the heart muscle. This zoonotic species has also been identified in cattle from the Netherlands (1%) and Turkey [4,10]. It should be noted that due to the different morphological, serological or molecular methods used in studies, it is difficult to compare the distribution of Sarcocystis species identified in cattle.…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, several trends in the prevalence of Sarcocystis species can be indicated. Most studies carried out in cattle reported the highest prevalence of S. cruzi employing canids as definitive hosts [10,14,15,18,37,[40][41][42][43][44]. By contrast, a relatively low prevalence of S. hirsuta using felids as definitive hosts was often recorded [14,15,37,41,45].…”

Section: Discussionmentioning

confidence: 99%

“…There has been much debate about the validity and classification of several Sarcocystis species from cattle, as well as their specificity to the intermediate host [5][6][7][8]. Current consensus is that that cattle may harbour up to six Sarcocystis species, namely S. bovifelis, S. bovini, S. cruzi, S. heydorni, S. hirsuta and S. hominis [4,[8][9][10][11]. As morphological analysis is not commonly sufficient to identify Sarcocystis species in cattle [12][13][14], the need for molecular methods is increasing.…”

mentioning

confidence: 99%

“…Sarcocystis spp. from cattle have been molecularly characterised at the genes for 18S rRNA (18S rRNA), 28S rRNA (28S rRNA) and cytochrome c oxidase subunit I (cox1) and at nuclear rDNA internal transcribed spacer 1 (ITS1) [8,10,11,15]. Databases contain many 18S rRNA gene sequences of the genus Sarcocystis used for species identification [16][17][18].…”

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Molecular identification of four Sarcocystis species in cattle from Lithuania, including S. hominis, and development of a rapid molecular detection method

et al. 2020

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Background Six Sarcocystis species are known to use cattle (Bos taurus) as the intermediate host, two of which, S. hominis and S. heydorni, are zoonotic. There is a need for a method that will enable rapid identification of the Sarcocystis species in cattle. Methods The diaphragm muscles of 102 cattle from Lithuania were examined for the presence of Sarcocystis spp., using two different methods for species identification. Individual sarcocysts were isolated from squash preparations of the diaphragm muscle under the light microscope, followed by genetic characterisation of excised cysts using sequence analysis of the 18S rRNA (18S rRNA) and cytochrome c oxidase subunit I (cox1) genes. The same cattle muscle samples were digested and species-specific PCR analyses targeting cox1 were developed to identify the Sarcocystis isolates to the species level. Results Under the light microscope, sarcocysts were detected in 87.3% of animals, and Sarcocystis infection was verified in all digested samples. Three species, namely S. cruzi (n = 20), S. bovifelis (n = 23) and S. hirsuta (n = 6), were identified by DNA sequence analysis of isolated sarcocysts. Based on sequence analysis of cox1, the level of genetic variability depended on Sarcocystis species and geographical location. Four Sarcocystis species, S. cruzi (96.1%), S. bovifelis (71.6%), S. hirsuta (30.4%) and S. hominis (13.7%), were confirmed in the digested samples. In individual samples, the most common finding was two species of Sarcocystis (44.1%), followed by three species (26.5%), a single species (24.5%) and four species (4.9%). Conclusions Although examination of tissue preparations under the light microscrope did not detect any sarcocysts belonging to S. hominis, this species was identified in the digested samples subjected to a cox1-specific PCR analysis. These results demonstrate the need for effective molecular diagnosis techniques to detect Sarcocystis spp., which may be present at a lower prevalence and not detectable among the limited number of sarcocysts identified individually under the light microscope.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Molecular identification of four Sarcocystis species in cattle from Lithuania, including S. hominis, and development of a rapid molecular detection method

et al. 2020

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“…2018; Hoeve‐Bakker et al . 2019). Some Sarcocystis species are specific to the intermediate host, while others have a wider range of hosts (Prakas et al .…”

Section: Introductionmentioning

confidence: 99%

Molecular phylogeny of Sarcocystis fayeri (Apicomplexa: Sarcocystidae) from the domestic horse Equus caballus based on 18S rRNA gene sequences and its prevalence

Abdel‐Gaber

Quraishy

Dkhil

et al. 2020

Lett Appl Microbiol

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Sarcocystosis is a parasitic disease caused by an intracellular protozoan parasite Sarcocystis belonging to the phylum Apicomplexa. These parasites have a requisite two‐host life cycle. Recently, there are many Sarcocystis species that identified morphologically. In the present study, diaphragmatic muscle samples from the domestic horse (Equus caballus) were examined for Sarcocystis infection. The natural infection with sarcocysts was recorded to be 62·5% for only microcysts in the infected muscles. Molecular analysis using the 18S rRNA gene was conducted to swiftly and accurately identify the recovered species. Studies on the expression of the 18S rRNA gene have confirmed that the present parasite isolates belong to the Sarcocystis genus. The sequence data showed significant identities (>80%) with archived gene sequences from species within the Sarcocystidae family, and a dendrogram showing the phylogenetic relationship was constructed. The most closely related species were the previously described Sarcocystis fayeri and Sarcocystis bertrami. The current data showed that the present species was identified as S. fayeri and deposited in GenBank (accession number MF614956.1). This study highlights the importance of the genetic data in the exact taxonomy within sarcocystid species.

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Molecular identification of Sarcocystis aucheniae in the wild South American camelid Vicugna vicugna

Wieser,

Cafrune,

Romero

et al. 2024

Vet Res Commun

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Molecular identification targeting cox1 and 18S genes confirms the high prevalence of Sarcocystis spp. in cattle in the Netherlands

Cited by 28 publications

References 33 publications

Molecular identification of four Sarcocystis species in cattle from Lithuania, including S. hominis, and development of a rapid molecular detection method

Molecular identification of four Sarcocystis species in cattle from Lithuania, including S. hominis, and development of a rapid molecular detection method

Molecular phylogeny of Sarcocystis fayeri (Apicomplexa: Sarcocystidae) from the domestic horse Equus caballus based on 18S rRNA gene sequences and its prevalence

Molecular identification of Sarcocystis aucheniae in the wild South American camelid Vicugna vicugna

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