Molecular Imaging of Biological Samples on Nanophotonic Laser Desorption Ionization Platforms

Stopka, Sylwia A.; Rong, Charles; Korte, Andrew R.; Yadavilli, Sridevi; Nazarian, Javad; Razunguzwa, Trust T.; Morris, Nicholas J.; Vertes, Akos

doi:10.1002/anie.201511691

Cited by 93 publications

(88 citation statements)

References 47 publications

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“…Atmospheric pressure MALDI‐MSI shows its ability for tissue imaging with sub‐micrometer resolution 5. Ambient ionization mass spectrometry techniques, such as desorption electrospray ionization (DESI) and laser ablation electrospray ionization (LAESI), have been developed for direct tissue imaging 6. Recently, an automated and biocompatible handheld mass spectrometry device, named MasSpec Pen was developed for rapid and nondestructive cancer diagnosis 7.…”

Section: Introductionmentioning

confidence: 99%

A Sensitive and Wide Coverage Ambient Mass Spectrometry Imaging Method for Functional Metabolites Based Molecular Histology

Sun

et al. 2018

Advanced Science

106

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Histological examination with a deep link between functional metabolites and tissue structure and biofunctions will provide important in situ biochemical information, and then essentially reveal what has happened in tissue at the molecular level. However, due to the complexity and heterogeneity of tissue samples and the large number of metabolites, it is still a challenge to globally map the diverse metabolites, especially for those low‐abundance functional ones. Here, a sensitive air flow‐assisted desorption electrospray ionization mass spectrometry imaging method for the mapping of a broad range of metabolites is presented. It exhibits properties characteristic of wide coverage, high sensitivity, wide dynamic range, rapid analysis procedure, and high specificity for tissue metabolites imaging. More than 1500 metabolites, including cholines, polyamines, amino acids, carnitines, nucleosides, nucleotides, nitrogen bases, organic acids, carbohydrates, cholesterol sulfate, cholic acid, lipids, etc., can be visualized in an untargeted analysis. The distribution of metabolites shows good spatial match with tissue histological structure and biofunctions in heterogeneous rat kidney, rat brain, and human esophageal cancer tissue. This method possesses the ability to globally showcase the molecular processes in tissue, and provide an insightful way for structural and functional molecular recognition in histological examination, even for intraoperative decision‐making.

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Section: Introductionmentioning

confidence: 99%

A Sensitive and Wide Coverage Ambient Mass Spectrometry Imaging Method for Functional Metabolites Based Molecular Histology

Sun

et al. 2018

Advanced Science

106

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“…Matrix-assisted laser desorption/ionization (MALDI) MS has displayed great superiority considering its fast analysis of analytes in seconds, accurate mass measurement for identification, simple sample preparation for broad application, and high sensitivity with low costs for practical use 1,2,5 . Inorganic particles (e.g., silicon 10,11 , carbon 12,13 , metal [14][15][16] , and metal oxide 17,18 ) have been preferred matrices in LDI MS for small metabolites as reported by research groups globally including ours. These particles can control the analytical variations and background signals in the mass region below m/z of 500, achieving the qualitative analysis of small metabolites, such as carbohydrates and amino acids 6,7,19 .…”

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confidence: 66%

Plasmonic silver nanoshells for drug and metabolite detection

et al. 2017

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In-vitro metabolite and drug detection rely on designed materials-based analytical platforms, which are universally used in biomedical research and clinical practice. However, metabolic analysis in bio-samples needs tedious sample preparation, due to the sample complexity and low molecular abundance. A further challenge is to construct diagnostic tools. Herein, we developed a platform using silver nanoshells. We synthesized SiO2@Ag with tunable shell structures by multi-cycled silver mirror reactions. Optimized nanoshells achieved direct laser desorption/ionization mass spectrometry in 0.5 μL of bio-fluids. We applied these nanoshells for disease diagnosis and therapeutic evaluation. We identified patients with postoperative brain infection through daily monitoring and glucose quantitation in cerebrospinal fluid. We measured drug distribution in blood and cerebrospinal fluid systems and validated the function of blood-brain/cerebrospinal fluid-barriers for pharmacokinetics. Our work sheds light on the design of materials for advanced metabolic analysis and precision diagnostics.

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“…These surfaces also do not display any matrix background, but they have a significant limitation for tissue analyses; the laser beam must penetrate the tissue before it can be absorbed by the underlying nanopore surface. This generally requires the tissue to be very thin (< 5 μm), although NAPA was successfully demonstrated for 10 μm thick tissue imaging . Bionanostructured surfaces, such as those from microalgae cell walls or commercialized celite, have also been used as matrices in LDI‐MS …”

Section: Development Of New Matrices For High‐spatial Resolution Imagingmentioning

confidence: 99%

“…This generally requires the tissue to be very thin (< 5 mm), although NAPA was successfully demonstrated for 10 mm thick tissue imaging. [47] Bionanostructured surfaces, such as those from microalgae cell walls or commercialized celite, have also been used as matrices in LDI-MS. [48] 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52…”

Section: Studies By Others For Small Molecule Matricesmentioning

confidence: 99%

High‐Spatial Resolution Mass Spectrometry Imaging: Toward Single Cell Metabolomics in Plant Tissues

Hansen

Lee

2017

The Chemical Record

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Mass spectrometry imaging (MSI) is a powerful tool that has advanced our understanding of complex biological processes by enabling unprecedented details of metabolic biology to be uncovered. Through the use of high-spatial resolution MSI, metabolite localizations can be obtained with high precision. Here we describe our recent progress to enhance the spatial resolution of matrix-assisted laser desorption/ionization (MALDI) MSI from ∼50 μm with the commercial configuration to ∼5 μm. Additionally, we describe our efforts to develop a 'multiplex MSI' data acquisition method to allow more chemical information to be obtained on a single tissue in a single instrument run, and the development of new matrices to improve the ionization efficiency for a variety of small molecule metabolites. In combination, these contributions, along with the efforts of others, will bring MSI experiments closer to achieving metabolomic scale.

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Molecular Imaging of Biological Samples on Nanophotonic Laser Desorption Ionization Platforms

Cited by 93 publications

References 47 publications

A Sensitive and Wide Coverage Ambient Mass Spectrometry Imaging Method for Functional Metabolites Based Molecular Histology

A Sensitive and Wide Coverage Ambient Mass Spectrometry Imaging Method for Functional Metabolites Based Molecular Histology

Plasmonic silver nanoshells for drug and metabolite detection

High‐Spatial Resolution Mass Spectrometry Imaging: Toward Single Cell Metabolomics in Plant Tissues

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