Molecular Imaging of Cardiovascular Disease

Verjansr, J.; Hofstra, Leonard

doi:10.24170/4-2-2098

Cited by 1 publication

(2 citation statements)

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“…Molecular imaging can visualize cellular functions and track in vivo molecular processes by non-invasive methods [39,40]. Radionuclide reporter gene imaging is a relatively mature molecular imaging method that enables accurate and non-invasive monitoring of therapeutic genes and cells quantitatively and repetitively [14,16,17,21]. 18 F-FHBG/HSV1-TK PET imaging is currently the most common reporter gene system [14].…”

Section: Discussionmentioning

confidence: 99%

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Visualization of gene therapy with a liver cancer-targeted adeno-associated virus 3 vector

Liu¹,

Huang²,

Gao³

et al. 2020

J. Cancer

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Background: To evaluate the feasibility of a self-complementing recombinant adeno-associated virus 3 (scrAAV3) vector targeting liver cancer and non-invasively monitor gene therapy of liver cancer. Materials and methods: An scrAAV3-HSV1-TK-kallistatin (ATK) gene drug was constructed, which contained the herpes virus thymidine kinase (HSV1-TK) reporter gene and human endogenous angiogenesis inhibitor (kallistatin) gene for non-invasive imaging of gene expression. Subcutaneous xenografted tumors of hepatoma in nude mice were generated for positron emission tomography/computed tomography (PET/CT) imaging. The ATK group was injected with the ATK gene through the tail vein, and an imaging agent was injected 2 weeks later. PET/CT imaging was performed at 1 hour after injection of the imaging agent. The control group was injected with phosphate-buffered saline at the same volume as the ATK gene drug. HE staining is used for pathological observation of tumor sections. HSV1-TK and kallistatin expression was identified by immunofluorescence, real-time quantitative PCR, and western blotting. Results: Radioactivity on PET/CT images was significantly higher in the ATK group compared with the control group. 18F-FHBG uptake values of left forelegs in ATK and control groups were 0.591±0.151% and 0.017 ± 0.011% ID/g (n=5), respectively (P<0.05). After injection of the ATK gene drug, mRNA and protein expression of HSV1-TK and kallistatin in subcutaneous xenograft tumors was detected successfully. In vitro analysis demonstrated significant differences in the expression of HSV1-TK and kallistatin between ATK and control groups (P<0.05). Conclusions: The scrAAV3 vector has a strong liver cancer-targeting ability, and the ATK gene drug can be used for targeted and non-invasive monitoring of liver cancer gene therapy.

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Section: Discussionmentioning

confidence: 99%

“…Reporter gene imaging is one of the more mature molecular imaging methods [16,17]. It introduces an exogenous gene (reporter gene) into cells, and the reporter gene expresses specific products such as enzymes, receptor proteins, or transporters.…”

Section: Ivyspringmentioning

confidence: 99%