Molecular Imaging of Steroid-Induced Osteonecrosis of the Femoral Head through iRGD-Targeted Microbubbles

Zhao, Ping; Zhao, Shuai; Zhang, Jiaqi; Lai, Manlin; Sun, Litao; Yan, Fei

doi:10.3390/pharmaceutics14091898

Cited by 4 publications

(1 citation statement)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Early diagnosis and treatment can prevent the progression of joint destruction, reduce the implementation of traumatic surgery, and improve the probability of hip preservation, such as some lncRNA, ultrasound molecular imaging, core decompression, osteotomy and physical therapy, etc. However, these methods are usually not very satisfactory, approximately 40% of patients progressing to total hip arthroplasty [5][6][7][8]. MSCs are considered to be an ideal material for bone regeneration and vascular regeneration because of its differentiation potency [9,10].…”

Section: Introductionmentioning

confidence: 99%

Runx2 overexpression promotes bone repair of osteonecrosis of the femoral head (ONFH)

Liu

et al. 2023

Mol Biol Rep

View full text Add to dashboard Cite

Background Runt-related transcription factor-2 (Runx2) has been considered an inducer to improve bone repair ability of mesenchymal stem cells (MSCs). Methods and results Twenty-four rabbits were used to establish Osteonecrosis of the femoral head (ONFH) and randomly devided into four groups: Adenovirus Runx2 (Ad-Runx2) group, Runx2-siRNA group, MSCs group and Model group. At 1 week after model establishment, the Ad-Runx2 group was treated with 5 × 107 MSCs transfected through Ad-Runx2, the Runx2-siRNA group was treated with 5 × 107 MSCs transfected through Runx2-siRNA, the MSCs group was injected with 5 × 107 untreated MSCs, and the Model group was treated with saline. The injection was administered at 1 week and 3 weeks after model establishment. The expression of bone morphogenetic protein 2 (BMP-2), Runx2 and Osterix from the femoral head was detected at 3 and 6 weeks after MSCs being injected, and Masson Trichrome Staining, Gross Morphology, X-ray and CT images observation were used to evaluate the repair effect of ONFH. The data revealed that the expression of BMP-2, Runx2 and Osterix in the Runx2-siRNA group was reduced at 3 weeks compared with the MSCs group, and then the expression further reduced at 6 weeks, but was still higher than the Model group besides Osterix; The expression of these three genes in the Ad-Runx2 group was higher than in the MSCs group. Masson Trichrome Staining, Gross Morphology and X-ray and CT images observation revealed that necrotic femoral head of the MSCs group was more regular and smooth than the Runx2-siRNA group, which has a collapsed and irregular femoral head. In the Ad-Runx2 group, necrotic femoral head was basically completely repaired and covered by rich cartilage and bone tissue. Conclusions Overexpression of Runx2 can improve osteoblastic phenotype maintenance of MSCs and promote necrotic bone repair of ONFH.

show abstract

Section: Introductionmentioning

confidence: 99%

Runx2 overexpression promotes bone repair of osteonecrosis of the femoral head (ONFH)

Liu

et al. 2023

Mol Biol Rep

View full text Add to dashboard Cite

show abstract

Cancer therapy with iRGD as a tumor-penetrating peptide

Thirumalai,

Girigoswami,

Pallavi

et al. 2023

Bulletin du Cancer

View full text Add to dashboard Cite

Toward Ultrasound Molecular Imaging of Endothelial Dysfunction in Diabetes: Targets, Strategies, and Challenges

Liu,

Wang,

Qiu

et al. 2024

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

Diabetes vasculopathy is a significant complication of diabetes mellitus (DM), and early identification and timely intervention can effectively slow the progression. Accumulating studies have shown that diabetes causes vascular complications directly or indirectly through a variety of mechanisms. Direct imaging of the endothelial molecular changes not only identifies the early stage of diabetes vasculopathy but also sheds light on the precise treatment. Targeted ultrasound contrast agent (UCA)-based ultrasound molecular imaging (UMI) can noninvasively detect the expression status of molecular biomarkers overexpressed in the vasculature, thereby being a potential strategy for the diagnosis and treatment response evaluation of DM. Amounts of efforts have been focused on identification of the molecular targets expressed in the vasculature, manufacturing strategies of the targeted UCA, and the clinical translation for the diagnosis and evaluation of therapeutic efficacy in both micro-and macrovasculopathy in DM. This review summarizes the latest research progress on endothelium-targeted UCA and discusses their promising future and challenges in diabetes vasculopathy theranostics.

show abstract

Molecular Imaging of Steroid-Induced Osteonecrosis of the Femoral Head through iRGD-Targeted Microbubbles

Cited by 4 publications

References 50 publications

Runx2 overexpression promotes bone repair of osteonecrosis of the femoral head (ONFH)

Runx2 overexpression promotes bone repair of osteonecrosis of the femoral head (ONFH)

Cancer therapy with iRGD as a tumor-penetrating peptide

Toward Ultrasound Molecular Imaging of Endothelial Dysfunction in Diabetes: Targets, Strategies, and Challenges

Contact Info

Product

Resources

About