Molecular imprinting of miR-559 on a peptide-immobilized poly L-DOPA/silica core–shell and in vitro investigating its effects on HER2-positive breast cancer cells

Mohammadzade, Hadi; Hashemi‐Moghaddam, Hamid; Beikzadeh, Leila; Ahmadieh-Yazdi, Amirhossein; Madanchi, Hamid; Fallah, Parviz

doi:10.1007/s13346-023-01330-x

Cited by 6 publications

(5 citation statements)

References 46 publications

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“…The selectivity of the nrCap18 to the cells to the cancer cell membrane (MDA-MB 231 cell line) was evaluated by the MTT assay with some modification called MTT after the washing method, which was previously explained by Madnachi et al 25 nrCap18 peptide has a high affinity to the negative charge cell membranes (cancer cells) due to its high cationic property, meanwhile, it has less affinity to normal cells. At first, the MDA-MB 231 and MCF-10A cells were cultured in a 96-well plate overnight.…”

Section: Methodsmentioning

confidence: 99%

“…After an hour of treatment, the wells were drained and washed twice with PBS. Next, the complete medium was added to wells, and the incubation continued for 24 h. Finally, other steps were performed as in a standard MTT method 25 .…”

Section: Methodsmentioning

confidence: 99%

“…A flow cytometer by BD FACSCalibur (BD Biosciences, California, USA) was used to analyze the samples. Annexin V + , 7-AAD − cells were considered in the early stage of apoptosis, while Annexin V + , 7-AAD + cells were supposed to be in the late stage of apoptosis 25 . At last, data was analyzed by FlowJo software (version 10, TreeStar, USA).…”

Section: Methodsmentioning

confidence: 99%

“…1 ml of PI staining solution at 50 μg/mL was added to the cell pellet. In the following, a final concentration of 0.5 μg/mL in 50 μL of RNase A stock solution was added to samples and were then incubated at 4 °C for at least 4 h. 106 events from stored samples kept at 4 °C were examined using flow cytometry 25 .…”

Section: Methodsmentioning

confidence: 99%

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Investigation of cytotoxic effect and action mechanism of a synthetic peptide derivative of rabbit cathelicidin against MDA-MB-231 breast cancer cell line

Bashi,

Madanchi,

Yousefi

2024

Sci Rep

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Antimicrobial peptides (AMPs) have sparked significant interest as potential anti-cancer agents, thereby becoming a focal point in pursuing novel cancer-fighting strategies. These peptides possess distinctive properties, underscoring the importance of developing more potent and selectively targeted versions with diverse mechanisms of action against human cancer cells. Such advancements would offer notable advantages compared to existing cancer therapies. This research aimed to examine the toxicity and selectivity of the nrCap18 peptide in both cancer and normal cell lines. Furthermore, the rate of cellular death was assessed using apoptosis and acridine orange/ethidium bromide (AO/EB) double staining at three distinct incubation times. Additionally, the impact of this peptide on the cancer cell cycle and migration was evaluated, and ultimately, the expression of cyclin-dependent kinase 4/6 (CDK4/6) genes was investigated. The results obtained from the study demonstrated significant toxicity and selectivity in cancer cells compared to normal cells. Moreover, a strong progressive increase in cell death was observed over time. Furthermore, the peptide exhibited the ability to halt the progression of cancer cells in the G1 phase of the cell cycle and impede their migration by suppressing the expression of CDK4/6 genes.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Investigation of cytotoxic effect and action mechanism of a synthetic peptide derivative of rabbit cathelicidin against MDA-MB-231 breast cancer cell line

Bashi,

Madanchi,

Yousefi

2024

Sci Rep

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“…Due to the excellent adhesive properties of polydopamine, it has been extensively used as a coating agent for various applications, including functionalization, sensing, and drug delivery 18 . Polymerization of L-DOPA on the surface of silica nanoparticles leads to drug retention, prevention of degradation, and controlled release 19 .…”

Section: Introductionmentioning

confidence: 99%

Bromelain-loaded nanocomposites decrease inflammatory and cytotoxicity effects of gliadin on Caco-2 cells and peripheral blood mononuclear cells of celiac patients

Mousavi Maleki,

Ebrahimi kiasari,

Seyed Mousavi

et al. 2023

Sci Rep

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Enzyme therapy can be an appropriate treatment option for celiac disease (CeD). Here, we developed Bromelain-Loaded Nanocomposites (BLNCs) to improve the stability and retention of bromelain enzyme activity. After the characterization of BLNCs, the cytotoxicity of BLNCs was determined on the Caco-2 cell line. The effect of BLNCs on gliadin degradation and the production of pro-inflammatory cytokines and anti-inflammatory molecules in peripheral blood mononuclear cells (PBMCs) obtained from celiac patients were assessed. Furthermore, the expression of CXCR3 and CCR5 genes was measured in CaCo-2 cells treated with gliadin, gliadin-digested with BLNCs, and bromelain. Our study demonstrated that the Bromelain entrapment efficiency in these nanoparticles was acceptable, and BLNCs have no toxic effect on cells. SDS-PAGE confirmed the digestion effect of bromelain released from nanocomposites. When Caco-2 cells were treated with gliadin digested by free bromelain and BLNCs, the expression of CXCR3 and CCR5 genes was significantly decreased. PBMCs of celiac patients treated with Bromelain and BLNCs decreased inflammatory cytokines (IL-1β, IL-6, TNF-α, and IFN-γ) production compared to untreated PBMCs. This treatment also increased IL-10 and CTLA-4 in PBMCs of CeD patients. According to the promising results of this study, we can hope for the therapeutic potential of BLNCs for CeD.

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Bromelain and ficin proteolytic effects on gliadin cytotoxicity and expression of genes involved in cell-tight junctions in Caco-2 cells

Mousavi Maleki,

Aghamirza Moghim Ali Abadi,

Vaziri

et al. 2023

Amino Acids

Self Cite

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Enzyme therapy for Celiac disease (CeD), which digests gliadin into non-immunogenic and non-toxic peptides, can be an appropriate treatment option for CeD. Here we have investigated the effectiveness of Bromelain and Ficin on gliadin digestion using in vitro such as SDS-PAGE, HPLC, and circular dichroism (CD). Furthermore, the cytotoxicity of gliadin and 19-mer peptide before and after digestion with these enzymes was evaluated using the MTT assay in the Caco-2 cell line. Finally, we examined the effect of these treatments along with Larazotide Acetate (LA) on the expression of genes involved in cell tight junctions such as Occludin (OCCL), Claudin 3 (CLDN), Tight junction protein-1 (TGP), and Zonulin (ZON) in the Caco-2 cell line. Our study demonstrated Bromelain and Ficin digestion effects on the commercial and wheat-extracted gliadin by SDS-PAGE, HPLC, and Circular Dichroism (CD). Also, the cytotoxicity results on Caco-2 showed that toxicity of the gliadin and synthetic 19-mer peptide was decreased by adding Bromelain and Ficin. Furthermore, the proteolytic effects of Bromelain and Ficin on gliadin indicated the expression of genes involved in cell-tight junctions was improved. This study con rms that Bromelain and Ficin mixture could be effective in improving the symptoms of CeD.

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Molecular imprinting of miR-559 on a peptide-immobilized poly L-DOPA/silica core–shell and in vitro investigating its effects on HER2-positive breast cancer cells

Cited by 6 publications

References 46 publications

Investigation of cytotoxic effect and action mechanism of a synthetic peptide derivative of rabbit cathelicidin against MDA-MB-231 breast cancer cell line

Investigation of cytotoxic effect and action mechanism of a synthetic peptide derivative of rabbit cathelicidin against MDA-MB-231 breast cancer cell line

Bromelain-loaded nanocomposites decrease inflammatory and cytotoxicity effects of gliadin on Caco-2 cells and peripheral blood mononuclear cells of celiac patients

Bromelain and ficin proteolytic effects on gliadin cytotoxicity and expression of genes involved in cell-tight junctions in Caco-2 cells

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