Molecular Imprinting Techniques Used for the Preparation of Biosensors

Ertürk, Gizem; Mattìasson, Bo

doi:10.3390/s17020288

Cited by 219 publications

(114 citation statements)

References 66 publications

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“…Micro-contact imprinting is considered as one of the promising techniques for protein imprinting [61] that can address its common associated problems such as solubility, conformational stability, and aggregation during the polymerization process [62]. This approach allows rapid fabrication of MIPs using small amounts of template, and monomer solution with a possibility to polymerize dozens of samples at the same time applying the same polymerization batch [62][63][64][65]. In a typical procedure, the template is firstly immobilized or adsorbed on a support (glass) to create the protein stamp.…”

Section: Imprinting Of Proteinsmentioning

confidence: 99%

Strategies for Molecular Imprinting and the Evolution of MIP Nanoparticles as Plastic Antibodies—Synthesis and Applications

Refaat

Aggour

Farghali

et al. 2019

IJMS

143

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Materials that can mimic the molecular recognition-based functions found in biology are a significant goal for science and technology. Molecular imprinting is a technology that addresses this challenge by providing polymeric materials with antibody-like recognition characteristics. Recently, significant progress has been achieved in solving many of the practical problems traditionally associated with molecularly imprinted polymers (MIPs), such as difficulties with imprinting of proteins, poor compatibility with aqueous environments, template leakage, and the presence of heterogeneous populations of binding sites in the polymers that contribute to high levels of non-specific binding. This success is closely related to the technology-driven shift in MIP research from traditional bulk polymer formats into the nanomaterial domain. The aim of this article is to throw light on recent developments in this field and to present a critical discussion of the current state of molecular imprinting and its potential in real world applications.

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Section: Imprinting Of Proteinsmentioning

confidence: 99%

Strategies for Molecular Imprinting and the Evolution of MIP Nanoparticles as Plastic Antibodies—Synthesis and Applications

Refaat

Aggour

Farghali

et al. 2019

IJMS

143

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“…Bulk imprinting is a method of preparing a MIP via bulk polymerization to form a polymer followed by pulverization to form small particles . As early as the 1990s, there was related report on the bulk imprinting for amino acids.…”

Section: Biological Molecular Imprinting With Different Scalementioning

confidence: 99%

“…[114] Bulk Imprinting: Bulk imprinting is a method of preparing a MIP via bulk polymerization to form a polymer followed by pulverization to form small particles. [115] As early as the 1990s, there was related report on the bulk imprinting for amino acids. Although bulk imprinting is a simple and straight forward method, it inevitably has some drawbacks like lim ited mobility and low specificity to template molecules even for amino acids with simple mole cular structure.…”

Section: Amino Acids Imprintingmentioning

confidence: 99%

Molecularly Imprinted Materials for Selective Biological Recognition

Zhang

et al. 2019

Macromol. Rapid Commun.

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Molecular imprinting is an approach of generating imprinting cavities in polymer structures that are compatible with the target molecules. The cavities have memory for shape and chemical recognition, similar to the recognition mechanism of antigen–antibody in organisms. Their structures are also called biomimetic receptors or synthetic receptors. Owing to the excellent selectivity and unique structural predictability of molecularly imprinted materials (MIMs), practical MIMs have become a rapidly evolving research area providing key factors for understanding separation, recognition, and regenerative properties toward biological small molecules to biomacromolecules, even cell and microorganism. In this review, the characteristics, morphologies, and applicability of currently popular carrier materials for molecular imprinting, especially the fundamental role of hydrogels, porous materials, hierarchical nanoparticles, and 2D materials in the separation and recognition of biological templates are discussed. Moreover, through a series of case studies, emphasis is given on introducing imprinting strategies for biological templates with different molecular scales. In particular, the differences and connections between small molecular imprinting (bulk imprinting, “dummy” template imprinting, etc.), large molecular imprinting (surface imprinting, interfacial imprinting, etc.), and cell imprinting strategies are demonstrated in detail. Finally, future research directions are provided.

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“…Molecularly imprinted polymers (MIPs) are popular synthetic receptors that are widely used in designing selective sensors for targeted application . Some of the attractive advantages of using MIPs is that they are easy to prepare, cost effective, and have high mechanical and chemical stability, and can be tailored towards an analyte of interest.…”

Section: Introductionmentioning

confidence: 99%

Development of a quantum dot molecularly imprinted polymer sensor for fluorescence detection of atrazine

Nsibande

Forbes

2019

Luminescence

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Atrazine is a common agricultural pesticide which has been reported to occur widely in surface drinking water, making it an environmental pollutant of concern. In the quest for developing sensitive detection methods for pesticides, the use of quantum dots (QDs) as sensitive fluorescence probes has gained momentum in recent years.QDs have attractive and unique optical properties whilst coupling of QDs to molecularly imprinted polymers (MIPs) has been shown to offer excellent selectivity. Thus, the development of QD@MIPs based fluorescence sensors could provide an alternative for monitoring herbicides like atrazine in water. In this work, highly fluorescent CdSeTe/ZnS QDs were fabricated using the conventional organometallic synthesis approach and were then encapsulated with MIPs. The CdSeTe/ZnS@MIP sensor was characterized and applied for selective detection of atrazine. The sensor showed a fast response time (5 min) upon interaction with atrazine and the fluorescence intensity was linearly quenched within the 2-20 mol L −1 atrazine range. The detection limit of 0.80 × 10 −7 mol L −1 is comparable to reported environmental levels. Lastly, the sensor was applied in real water samples and showed satisfactory recoveries (92-118%) in spiked samples, hence it is a promising candidate for use in water monitoring. KEYWORDS atrazine, fluorescence sensor, molecularly imprinted polymer, quantum dot

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Molecular Imprinting Techniques Used for the Preparation of Biosensors

Cited by 219 publications

References 66 publications

Strategies for Molecular Imprinting and the Evolution of MIP Nanoparticles as Plastic Antibodies—Synthesis and Applications

Strategies for Molecular Imprinting and the Evolution of MIP Nanoparticles as Plastic Antibodies—Synthesis and Applications

Molecularly Imprinted Materials for Selective Biological Recognition

Development of a quantum dot molecularly imprinted polymer sensor for fluorescence detection of atrazine

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