Molecular insight into somaclonal variation phenomena from transcriptome profiling of cucumber (Cucumis sativus L.) lines

Pawełkowicz, Magdalena; Skarzyńska, Agnieszka; Mróz, Tomasz; Bystrzycki, Estelle; Pląder, Wojciech

doi:10.1007/s11240-020-02005-y

Cited by 16 publications

(15 citation statements)

References 68 publications

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“…Variations in gene expressions in tissue-culture-derived plants have also been documented in Phalaenopsis ‘Wedding Promenade’ [ 46 ] and rice [ 47 ]. When investigating the fruit transcriptome of cucumber somaclones, Pawe\lkowicz et al [ 48 ] documented a different differential gene expression in each clone. The authors attributed the results to variations in the genic region and the interactions among molecular networks, which initiate specific pathways.…”

Section: Discussionmentioning

confidence: 99%

Somaclonal Variation for Genetic Improvement of Starch Accumulation in Potato (Solanum tuberosum) Tubers

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Niedbała

El-Denary

et al. 2023

Plants

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Starch content is one of the major quality criteria targeted by potato breeding programs. Traditional potato breeding is a laborious duty due to the tetraploid nature and immense heterozygosity of potato genomes. In addition, screening for functional genetic variations in wild relatives is slow and strenuous. Moreover, genetic diversity, which is the raw material for breeding programs, is limited due to vegetative propagation used in the potato industry. Somaclonal variation provides a time-efficient tool to breeders for obtaining genetic variability, which is essential for breeding programs, at a reasonable cost and independent of sophisticated technology. The present investigation aimed to create potato somaclones with an improved potential for starch accumulation. Based on the weight and starch content of tubers, the somaclonal variant Ros 119, among 105 callus-sourced clones, recorded a higher tuberization potential than the parent cv Lady Rosetta in a field experiment. Although this somaclone was similar to the parent in the number of tubers produced, it exhibited tubers with 42 and 61% higher fresh and dry weights, respectively. Additionally, this clone recorded 10 and 75% increases in starch content based on the dry weight and average content per plant, respectively. The enhanced starch accumulation was associated with the upregulation of six starch-synthesis-related genes, namely, the AGPase, GBSS I, SBE I, SBE II, SS II and SS III genes. AGPase affords the glycosyl moieties required for the synthesis of amylose and amylopectin. GBSS is required for amylose elongation, while SBE I, SBE II, SS II and SS III are responsible for amylopectin.

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Section: Discussionmentioning

confidence: 99%

Somaclonal Variation for Genetic Improvement of Starch Accumulation in Potato (Solanum tuberosum) Tubers

Adly

Niedbała

El-Denary

et al. 2023

Plants

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“…The in vitro micropropagation technique has a lot of benefits; the most important one is represented in producing true-to-type plantlets, which are genetically and physiologically uniform. These plant materials can be used in the intensive production of several plants such as cucumber (Cucumis sativus L.) [24], olive trees (Olea europaea L.) [49], strawberry (Fragaria × ananassa Duchesne) [50], cardamom (Elettaria cardamomum Maton) [51], and Flemingia macrophylla (Willd.) Merr [52].…”

Section: Delay Of Subculture and Burned Plantletsmentioning

confidence: 99%

“…The somaclonal variation point to culture-induced, unexpected, and undesired variations or anomalies, which eventually is inherited by the clonal progenies, becomes genetic and happens during tissue cultures; this is, thus, noticed in the in vitro regenerated plants, and is an often an unwanted phenomenon, especially in mass micropropagation programs that are highly desirable for the production of plant material of the same type as the mother (genetically uniform) by maintaining the genetic stability of the in vitro regenerated plantlets [24,25]. Somaclonal variation is genotype-dependent, where some genotypes or species are more amenable to these changes than others when they are cultured in vitro [87].…”

Section: Problems Originated Due To Genetical Reasons Somaclonal Vari...mentioning

confidence: 99%

“…These nurseries have huge economic importance for many horticultural production systems, e.g., seedling production for tree-growing programs [17]; availability of high-quality tree planting materials [18]; and disease screening and discovery of new plant pathogens to maintain healthy nurseries [19], with the aim of producing high-quality, productivity as well as pathogenfree plants and/or trees. However, there are many obstacles, which may cause a loss for in vitro cultured plants in nurseries and in vitro culture laboratories, such as contamination of cultures [20], hyperhydricity phenomenon [21], browning of tissues or phenols exudation [22], shoot tip necrosis [23], delay of subculture, somaclonal variations [24,25], root hardening [20], and failure of acclimatization or limited planting [26].…”

Section: Introductionmentioning

confidence: 99%

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An Academic and Technical Overview on Plant Micropropagation Challenges

et al. 2022

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The production of micropropagated plants in plant-tissue-culture laboratories and nurseries is the most important method for propagation of many economic plants. Micropropagation based on tissue-culture technology involves large-scale propagation, as it allows multiplication of a huge number of true-to-type propagules in a very short time and in a very limited space, as well as all year round, regardless of the climate. However, applying plant-tissue-culture techniques for the commercial propagation of plants may face a lot of obstacles or troubles that could result from technical, biological, physiological, and/or genetical reasons, or due to overproduction or the lack of facilities and professional technicians, as shown in the current study. Moreover, several disorders and abnormalities are discussed in the present review. This study aims to show the most serious problems and obstacles of plant micropropagation, and their solutions from both scientific and technical sides. This review, as a first report, includes different challenges in plant micropropagation (i.e., contamination, delay of subculture, burned plantlets, browning, in vitro rooting difficulty, somaclonal variations, hyperhydricity, shoot tip necrosis, albino plantlets, recalcitrance, shoot abnormalities, in vitro habituation) in one paper. Most of these problems are related to scientific and/or technical reasons, and they could be avoided by following the micropropagation protocol suitable for each plant species. The others are dominant in plant-tissue-culture laboratories, in which facilities are often incomplete, or due to poor infrastructure and scarce funds.

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“…A condition for the successful use of in vitro techniques for mass production is the maintenance of the genetic stability of a given genotype. It is well known that micropropagation may be accompanied by somaclonal variations resulting from permanent changes (genetic variation, e.g., point mutations or changes in the number or structure of chromosomes) or transient changes (epigenetic variability, changes in DNA methylation pattern) (Miler & Zalewska, 2014;Pawełkowicz et al, 2021). e degree of variation depends on the regeneration system, culture conditions, and genotype.…”

Section: Introductionmentioning

confidence: 99%

Application and Improvement of In Vitro Culture Systems for Commercial Production of Ornamental, Fruit, and Industrial Plants in Poland

et al. 2022

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This work focuses on the achievements of Polish researchers in the field of vegetative reproduction of plants under in vitro conditions. For more than 50 years, micropropagation methods have been used in Poland whenever vegetative reproduction is necessary. Most perennial horticultural crops, such as fruit plants, the majority of ornamental geophytes, and some vegetables (e.g., rhubarb and horseradish), require clonal reproduction owing to their high heterozygosity, i.e., their offsprings when reproduced from seeds, do not repeat the parental characteristics. Various goals have been pursued in the development of regenerative and in vitro propagation systems for each of the aforementioned groups of plants, such as pathogen elimination, reproduction of healthy plants, rapid multiplication of newly obtained valuable breeding lines and cultivars, and breeding to obtain polyploids, haploids, and doubled haploids. Owing to the growing interest of researchers and plant producers in environmentally friendly technologies, one of the sections is devoted to the issue of biotization of micropropagated plants.

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Molecular insight into somaclonal variation phenomena from transcriptome profiling of cucumber (Cucumis sativus L.) lines

Cited by 16 publications

References 68 publications

Somaclonal Variation for Genetic Improvement of Starch Accumulation in Potato (Solanum tuberosum) Tubers

Somaclonal Variation for Genetic Improvement of Starch Accumulation in Potato (Solanum tuberosum) Tubers

An Academic and Technical Overview on Plant Micropropagation Challenges

Application and Improvement of In Vitro Culture Systems for Commercial Production of Ornamental, Fruit, and Industrial Plants in Poland

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