Molecular insights into AabZIP1-mediated regulation on artemisinin biosynthesis and drought tolerance in Artemisia annua

Shu, Guoping; Yuan, Mingyuan; Wei, Ning; Zhang, Fangyuan; Yang, Chunxian; Lan, Xiaozhong; Chen, Min; Tang, Kexuan; Xiang, Lan; Liao, Zhihua

doi:10.1016/j.apsb.2021.09.026

Cited by 25 publications

(21 citation statements)

References 56 publications

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“…Differential expression profiles of TFs were analyzed. TFs have been identified in regulating the biosynthesis of terpene-specialized metabolites in different plants, and include AaWRKY, AaERF1, AaMYB1, AabZIP1 and MsYABBY5 ( Shu et al, 2022 ; Q. Wang et al, 2016 ).…”

Section: Resultsmentioning

confidence: 99%

Transcriptome sequencing and gas chromatography–mass spectrometry analyses provide insights into β-caryophyllene biosynthesis in Brassica campestris

Wang

Zong

Bai

et al. 2022

Food Chemistry: Molecular Sciences

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Section: Resultsmentioning

confidence: 99%

Transcriptome sequencing and gas chromatography–mass spectrometry analyses provide insights into β-caryophyllene biosynthesis in Brassica campestris

Wang

Zong

Bai

et al. 2022

Food Chemistry: Molecular Sciences

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“…Abscisic acid‐responsive AabHLH1 directly activates the promoters of ADS and CYP71AV1 to positively regulate the biosynthesis of artemisinin (Ji et al ., 2014; Li et al ., 2019). Jasmonic acid‐induced AaMYC2 directly activates the transcription of CYP71AV1 , DBR2 , and ALDH1 (Shen et al ., 2016; Shu et al ., 2022). AabHLH112, which responses to cold and JA (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, AabZIP1 directly binds to and activates ADS and CYP71AV1 promoters (Zhang et al, 2015). However, Aab-ZIP1 does not bind to any ABRE cis-elements on DBR2 and ALDH1 promoters (Shu et al, 2022), indicating that AabZIP1 requires other proteins to activate DBR2 and ALDH1 promoters. Shu et al (2022) reported that AaMYC2, a direct activator of DBR2 and ALDH1, works as a bridge between the AabZIP1 and DBR2/ALDH1 promoters (Shen et al, 2016;Shu et al, 2022).…”

Section: New Phytologistmentioning

confidence: 96%

AabHLH113 integrates jasmonic acid and abscisic acid signaling to positively regulate artemisinin biosynthesis in Artemisia annua

et al. 2022

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Summary Artemisinin, a sesquiterpene lactone isolated from Artemisia annua, is in huge market demand due to its efficient antimalarial action, especially after the COVID‐19 pandemic. Many researchers have elucidated that phytohormones jasmonic acid (JA) and abscisic acid (ABA) positively regulate artemisinin biosynthesis via types of transcription factors (TFs). However, the crosstalk between JA and ABA in regulating artemisinin biosynthesis remains unclear. Here, we identified a novel ABA‐ and JA‐induced bHLH TF, AabHLH113, which positively regulated artemisinin biosynthesis by directly binding to the promoters of artemisinin biosynthetic genes, DBR2 and ALDH1. The contents of artemisinin and dihydroartemisinic acid increased by 1.71‐ to 2.06‐fold and 1.47‐ to 2.23‐fold, respectively, in AabHLH1113 overexpressed A. annua, whereas they decreased by 14–36% and 26–53%, respectively, in RNAi‐AabHLH113 plants. Furthermore, we demonstrated that AabZIP1 and AabHLH112, which, respectively, participate in ABA and JA signaling pathway to regulate artemisinin biosynthesis, directly bind to and activate the promoter of AabHLH113. Collectively, we revealed a complex network in which AabHLH113 plays a key interrelational role to integrate ABA‐ and JA‐mediated regulation of artemisinin biosynthesis.

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Section: Production Of Artemisinin In Genetically Engineered Plantsmentioning

confidence: 99%

“…A recent study reported that AabZIP1 can also directly bind to the promoter of the AaMYC2 gene and up-regulate the expression of AaMYC2, while AaMYC2 bound to the promoters of the AaDBR2 and AaALDH1 genes, which indicated that AabZIP1 indirectly activated the expression of the AaDBR2 and AaALDH1 genes through AaMYC2 and further improved artemisinin biosynthesis [ 85 ]. In addition, AabZIP1 can directly activate the transcription of cuticle wax biosynthesis genes AaCER1 and AaCYP86A1 and enhance the drought resistance of A. annua [ 85 ]. The results showed that the AabZIP1–AaMYC2 transcriptional module was a cross-talk between the ABA and JA signaling pathways in artemisinin biosynthesis, which provided a useful candidate gene for the genetic breeding of A. annua with a high artemisinin content and strong drought resistance.…”

Section: Production Of Artemisinin In Genetically Engineered Plantsmentioning

confidence: 99%

From Plant to Yeast—Advances in Biosynthesis of Artemisinin

et al. 2022

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Malaria is a life-threatening disease. Artemisinin-based combination therapy (ACT) is the preferred choice for malaria treatment recommended by the World Health Organization. At present, the main source of artemisinin is extracted from Artemisia annua; however, the artemisinin content in A. annua is only 0.1–1%, which cannot meet global demand. Meanwhile, the chemical synthesis of artemisinin has disadvantages such as complicated steps, high cost and low yield. Therefore, the application of the synthetic biology approach to produce artemisinin in vivo has magnificent prospects. In this review, the biosynthesis pathway of artemisinin was summarized. Then we discussed the advances in the heterologous biosynthesis of artemisinin using microorganisms (Escherichia coli and Saccharomyces cerevisiae) as chassis cells. With yeast as the cell factory, the production of artemisinin was transferred from plant to yeast. Through the optimization of the fermentation process, the yield of artemisinic acid reached 25 g/L, thereby producing the semi-synthesis of artemisinin. Moreover, we reviewed the genetic engineering in A. annua to improve the artemisinin content, which included overexpressing artemisinin biosynthesis pathway genes, blocking key genes in competitive pathways, and regulating the expression of transcription factors related to artemisinin biosynthesis. Finally, the research progress of artemisinin production in other plants (Nicotiana, Physcomitrella, etc.) was discussed. The current advances in artemisinin biosynthesis may help lay the foundation for the remarkable up-regulation of artemisinin production in A. annua through gene editing or molecular design breeding in the future.

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Molecular insights into AabZIP1-mediated regulation on artemisinin biosynthesis and drought tolerance in Artemisia annua

Cited by 25 publications

References 56 publications

Transcriptome sequencing and gas chromatography–mass spectrometry analyses provide insights into β-caryophyllene biosynthesis in Brassica campestris

Transcriptome sequencing and gas chromatography–mass spectrometry analyses provide insights into β-caryophyllene biosynthesis in Brassica campestris

AabHLH113 integrates jasmonic acid and abscisic acid signaling to positively regulate artemisinin biosynthesis in Artemisia annua

From Plant to Yeast—Advances in Biosynthesis of Artemisinin

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