Molecular Insights into Freezing Stress in Peach Based on Multi-Omics and Biotechnology: An Overview

Muthuramalingam, Pandiyan; Shin, Hyunsuk; Adarshan, Sivakumar; Jeyasri, Rajendran; Priya, Arumugam; Chen, Jen‐Tsung; Ramesh, Manikandan

doi:10.3390/plants11060812

Cited by 16 publications

(10 citation statements)

References 132 publications

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“…Cold conditions, including freezing, are among the major abiotic stressors encountered by deciduous fruit trees, particularly peach ( Prunus persica ) trees. Peach is a highly prized, important seasonal fruit grown worldwide, including in South Korea ( Muthuramalingam et al., 2022 ). Over the past decade, owing to climate change and global warming, the peach blooming period has advanced by approximately 13 days ( https://data.kma.go.kr ).…”

Section: Introductionmentioning

confidence: 99%

Physiological and metabolic analyses reveal the proline-mediated flowering delay mechanism in Prunus persica

Park,

Muthuramalingam,

Jeong

et al. 2024

Front. Plant Sci.

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Peaches are susceptible to various environmental stresses. Particularly in late spring, freezing temperatures can damage peaches and consequently, affect their productivity. Therefore, flowering delay is a prominent strategy for avoiding spring frost damage. Our previous study confirmed that treatment with 5% sodium alginate and 100 mM CaCl2 (5AG) to avoid frost damage during the blooming stage delays flowering. To reveal the flowering delay mechanism of peaches, this study systematically analyzed the modification of amino acid profiles in control and 5AG-treated peach plants at different day intervals. Our findings indicate that arginine (Arg), glutamate (Glu), and proline (Pro) levels differed between the control and 5AG-treated peach shoots throughout the phenological development of flower buds. Furthermore, two amino acids (Arg and Glu) are involved in the Pro pathway. Thus, using a computational metabolomics method, Pro biosynthesis and its characteristics, gene ontology, gene synteny, cis-regulatory elements, and gene organizations were examined to decipher the involvement of Pro metabolism in peach flowering delay. In addition, qRT-PCR analysis revealed the transcriptional regulation of Pro-related and flowering-responsive genes and their role in flowering delay. Overall, this pilot study provides new insights into the role of Pro in the flowering delay mechanisms in Prunus persica through 5AG treatment.

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Section: Introductionmentioning

confidence: 99%

Physiological and metabolic analyses reveal the proline-mediated flowering delay mechanism in Prunus persica

Park,

Muthuramalingam,

Jeong

et al. 2024

Front. Plant Sci.

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“…In particular, when the temperature drops below 10–12 ℃, young plants begin to show cold stress symptoms on their leaves [ 3 , 4 ]. Several studies have shown that cold can directly affect photosynthetic potential and energy production, chlorophyll content, carotenoid content, increase in reactive oxygen species (ROS), while amino acid content is significantly reduced [ 5 – 7 ]. In addition to these biochemical attributes, transcriptome level studies have shown that cold tolerant genotypes exhibit higher gene expression for phytohormone-related genes, including jasmonic acid (JA), brassinosteroids (BRs) and others, MAPK signaling, and secondary metabolite biosynthesis [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…Cold-induced photoinhibition can affect fruit development [ 9 ]. Mango fruits exhibit pitting, rooting, darkening of lenticel, changes in fruit peel color, and pulp quality traits [ 5 ]. Cold stress can increase abortion of embryos [ 10 ] and change fruit firmness by modifying cell-wall related polymers [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Cold-stress induced metabolomic and transcriptomic changes in leaves of three mango varieties with different cold tolerance

Kong,

Hou,

Liu

et al. 2024

BMC Plant Biol

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Background Mango (Mangifera indica L.) is grown in Hainan, Guangdong, Yunnan, Sichuan, and Fujian provinces and Guanxi autonomous region of China. However, trees growing in these areas suffer severe cold stress during winter, which affects the yield. To this regard, data on global metabolome and transcriptome profiles of leaves are limited. Here, we used combined metabolome and transcriptome analyses of leaves of three mango cultivars with different cold stress tolerance, i.e. Jinhuang (J)—tolerant, Tainung (T) and Guiremang No. 82 (G)—susceptible, after 24 (LF), 48 (MF) and 72 (HF) hours of cold. Results A total of 1,323 metabolites belonging to 12 compound classes were detected. Of these, amino acids and derivatives, nucleotides and derivatives, and lipids accumulated in higher quantities after cold stress exposure in the three cultivars. Notably, Jinhuang leaves showed increasing accumulation trends of flavonoids, terpenoids, lignans and coumarins, and alkaloids with exposure time. Among the phytohormones, jasmonic acid and abscisic acid levels decreased, while N6-isopentenyladenine increased with cold stress time. Transcriptome analysis led to the identification of 22,526 differentially expressed genes. Many genes enriched in photosynthesis, antenna proteins, flavonoid, terpenoid (di- and sesquiterpenoids) and alkaloid biosynthesis pathways were upregulated in Jihuang leaves. Moreover, expression changes related to phytohormones, MAPK (including calcium and H2O2), and the ICE-CBF-COR signalling cascade indicate involvement of these pathways in cold stress responses. Conclusion Cold stress tolerance in mango leaves is associated with regulation of primary and secondary metabolite biosynthesis pathways. Jasmonic acid, abscisic acid, and cytokinins are potential regulators of cold stress responses in mango leaves.

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“…Since the first peach genome was reported in 2010, omics approaches (e.g., genomics, transcriptomics, proteomics, and metabolomics) have been widely used to investigate the roles of cold tolerance associated genes in peach ( Muthuramalingam et al., 2022 ). Digital expression analyses of EST datasets identified two promoters – Ppbec1 encoding endochitinase (C2131) and Ppxero2 encoding dehydrin (C254) as cold-inducible promoters for peach and reported the heterologous regulation of these promoters in peach at low temperatures ( Tittarelli et al., 2009 ).…”

Section: Introductionmentioning

confidence: 99%

Integrated analysis of transcriptomics and metabolomics of peach under cold stress

Tian

Wang

et al. 2023

Front. Plant Sci.

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Low temperature is one of the environmental factors that restrict the growth and geographical distribution of peach (Prunus persica L. Batsch). To explore the molecular mechanisms of peach brunches in response to cold, we analyzed the metabolomics and transcriptomics of ‘Donghe No.1’ (cold-tolerant, CT) and ‘21st Century’ (cold-sensitive, CS) treated by different temperatures (-5 to -30°C) for 12 h. Some cold-responsive metabolites (e.g., saccharides, phenolic acids and flavones) were identified with upregulation only in CT. Further, we identified 1991 cold tolerance associated genes in these samples and they were significantly enriched in the pathways of ‘galactose metabolism’, ‘phenylpropanoid biosynthesis’ and ‘flavonoids biosynthesis’. Weighted gene correlation network analysis showed that soluble sugar, flavone, and lignin biosynthetic associated genes might play a key role in the cold tolerance of peach. In addition, several key genes (e.g., COMT, CCR, CAD, PER and F3’H) were substantially expressed more in CT than CS under cold stress, indicating that they might be major factors during the adaptation of peach to low temperature. This study will not only improve our understanding towards the molecular mechanisms of peach trees under cold stress but also contribute to the screening and breeding program of peach in the future.

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Molecular Insights into Freezing Stress in Peach Based on Multi-Omics and Biotechnology: An Overview

Cited by 16 publications

References 132 publications

Physiological and metabolic analyses reveal the proline-mediated flowering delay mechanism in Prunus persica

Physiological and metabolic analyses reveal the proline-mediated flowering delay mechanism in Prunus persica

Cold-stress induced metabolomic and transcriptomic changes in leaves of three mango varieties with different cold tolerance

Integrated analysis of transcriptomics and metabolomics of peach under cold stress

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