Molecular insights into the activity and mechanism of cyanide hydratase enzyme associated with cyanide biodegradation by Serratia marcescens

Kushwaha, Madhulika; Kumar, Virender; Mahajan, Rishi; Bhalla, Tek Chand; Chatterjee, Subhankar; Akhter, Yusuf

doi:10.1007/s00203-018-1524-0

Cited by 8 publications

(3 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of a CynH was also suspected in the bacterium Serratia marcescens [26] after a reaction product corresponding to formamide was found in HPLC. The putative CynH sequence had a shorter amino acid chain than typical CynHs (326 residues).…”

Section: Introductionmentioning

confidence: 99%

Application Potential of Cyanide Hydratase from Exidia glandulosa: Free Cyanide Removal from Simulated Industrial Effluents

et al. 2021

View full text Add to dashboard Cite

Industries such as mining, cokemaking, (petro)chemical and electroplating produce effluents that contain free cyanide (fCN = HCN + CN−). Currently, fCN is mainly removed by (physico)chemical methods or by biotreatment with activated sludge. Cyanide hydratases (CynHs) (EC 4.2.1.66), which convert fCN to the much less toxic formamide, have been considered for a mild approach to wastewater decyanation. However, few data are available to evaluate the application potential of CynHs. In this study, we used a new CynH from Exidia glandulosa (protein KZV92691.1 designated NitEg by us), which was overproduced in Escherichia coli. The purified NitEg was highly active for fCN with 784 U/mg protein, kcat 927/s and kcat/KM 42/s/mM. It exhibited optimal activities at pH approximately 6–9 and 40–45 °C. It was quite stable in this pH range, and retained approximately 40% activity at 37 °C after 1 day. Silver and copper ions (1 mM) decreased its activity by 30–40%. The removal of 98–100% fCN was achieved for 0.6–100 mM fCN. Moreover, thiocyanate, sulfide, ammonia or phenol added in amounts typical of industrial effluents did not significantly reduce the fCN conversion, while electroplating effluents may need to be diluted due to high fCN and metal content. The ease of preparation of NitEg, its high specific activity, robustness and long shelf life make it a promising biocatalyst for the detoxification of fCN.

show abstract

Section: Introductionmentioning

confidence: 99%

Application Potential of Cyanide Hydratase from Exidia glandulosa: Free Cyanide Removal from Simulated Industrial Effluents

et al. 2021

View full text Add to dashboard Cite

show abstract

“…To date, several reports have described the ability of microorganisms to grow on cyanide compounds; bacterial communities such as Pseudomonas, capable of degrading cyanide 10 mg L -1 [10] and two isolates of this genus, immobilized on activated charcoal, degrading concentrations up to 340 mg L -1 [10,11] . Arthrobacter sp., Zoogloearamigera, Acidovorax sp., Achromobacter sp., Janthinobacterium sp., Klebsiella sp., Bacillus pumillus, Burkhoderia cepacia, Alcaligenes sp., Serratia marcescen and Rhodococcus sp., among others, have been reported as cyanide degrading organisms [6,[12][13][14][15][16][17][18][19]. Besides bacteria, species of the archaeal genus Methanosarcina (under anaerobic conditions), some fungi like Fusarium solani and Trichoderma polysporum at pH 4 [20] and some algae like Scenedesmus obliquus [21] have been reported to degrade cyanide.…”

Section: Introductionmentioning

confidence: 99%

Characterization of bacterial diversity and assessing the cyanide biodegradation potential of bacterial isolates from gold processing plants

López-Ramírez

Márquez-Godoy

Herrera

2021

DYNA

View full text Add to dashboard Cite

Cyanide is the basic component of many industrial processes, among which is gold processing, being very toxic or even lethal. Treatment, with the help of microorganisms, can be used effectively to reduce the load of harmful chemicals into the environment. The combination of microbiological methods and molecular tools allowed inferring the presence of a dominant population and the composition varied both in the places of origin and in the method used. The dominant phylogenetic affiliations of the bacteria were determined by sequencing the 16S rRNA gene. The isolates identified, as Bacillus and Enterococcus were capable to degrade 41.9 and 27.5 mg CN- L-1 respectively. This study provides information about the presence of a diverse bacterial community associated with residual effluents from cyanidation processes in Colombia and suggests that their presence could play a role in the biological degradation of cyanide compounds, offering an alternative for mining wastewater treatment.

show abstract

“…Another study showed that cyanide degradation by Serratia marcescens species forms formamide at the end which evidences the cyanide hydratase enzymatic pathway (KUSHWAHA et al, 2018).…”

Section: % 56%mentioning

confidence: 91%

Biological degradation of cyanide using native bacteria isolated from a cassava-processing effluent.

Arévalo¹,

Tania²

View full text Add to dashboard Cite

Cyanide (CN) can be present in the environment due to human activities including gold mining, electroplating, and nylon production. During the industrial processing of cassava, cyanide is liberated in effluents due to the presence of cyanogenic glycosides in the pulp. Cyanide is toxic for most living organisms since it inhibits the action of the cytochrome c oxidase and stops the electron transport chain during cellular respiration. Because of this, cyanide-containing effluents need to be treated prior to discharge to the environment, which can be achieved by chemical, physical or biological methods. In this context, this work evaluates the cyanide-degradation capacity of native bacteria isolated from a cassavaprocessing effluent and the best conditions for the biological removal of cyanide. In total, sixteen cyanide-resistant bacteria were isolated, identified by mass spectrometry technique MALDI-TOF, and tested individually for their ability to degrade cyanide. Eight different species were identified among the isolated strains. Four of them, belonging to the Klebsiella oxytoca, Bacillus pumilus, Corynebacterium glutamicum, and Serratia marcescens species, were selected for further assays to assess their susceptibility to pH and cyanide initial concentration. Results showed that cyanide degradation was inhibited when pH was above 9 and that higher initial cyanide concentrations resulted in lower cyanide degradation percentage. However, strains could still keep their degradation capacity even in high initial cyanide concentrations of 1000mg.L-1. Isolated Klebsiella oxytoca and Serratia marcescens were thus selected for optimization of the degradation process by analyzing the influence of carbon source, temperature and rotation speed. Both strains showed greater degradation rates when using sodium acetate as a carbon source and incubated at 30°C. Agitation speed did not show an effect on cyanide degradation by Klebsiella oxytoca, but enhanced degradation by Serratia marcescens strain. Under optimal incubation conditions, isolated Klebsiella oxytoca and Serratia marcescens strains could degrade cyanide from a synthetic 150mg.L-1 freecyanide solution, pH 8, using sodium acetate as a carbon source by 43 and 41% after 24hour of incubation, respectively, showing their potential to be used in the biodegradation of cyanide-containing effluents.

show abstract

Molecular insights into the activity and mechanism of cyanide hydratase enzyme associated with cyanide biodegradation by Serratia marcescens

Cited by 8 publications

References 25 publications

Application Potential of Cyanide Hydratase from Exidia glandulosa: Free Cyanide Removal from Simulated Industrial Effluents

Application Potential of Cyanide Hydratase from Exidia glandulosa: Free Cyanide Removal from Simulated Industrial Effluents

Characterization of bacterial diversity and assessing the cyanide biodegradation potential of bacterial isolates from gold processing plants

Biological degradation of cyanide using native bacteria isolated from a cassava-processing effluent.

Contact Info

Product

Resources

About