Molecular insights into the plasma membrane intrinsic proteins roles for abiotic stress and metalloids tolerance and transport in plants

Kumar, Kundan; Mosa, Kareem A.; Meselhy, Ahmed G.; Dhankher, Om Parkash

doi:10.1007/s40502-018-0425-1

Cited by 30 publications

(9 citation statements)

References 84 publications

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“…PIPs could mediate a broad boron uptake and distribution (Fitzpatrick and Reid 2009), however their co-function as water channels subjects them to tight regulation (Chaumont and Tyerman 2014). Boron-permeable PIPs would also provide tolerance to boron toxicity by enabling efflux of excess boron from roots and shoots (Kumar et al 2014; Kumar et al 2018). Unlike the PIPs, water-tight NIP IIs enable highly targeted boron transport in boron-limiting conditions, and are down-regulated in boron sufficient concentrations (Takano et al 2006; Chaumont and Tyerman 2017).…”

Section: Discussionmentioning

confidence: 99%

Exploring the Plant Aquaporin Solute Transport Network: Functional characterisation ofNicotiana tabacumPIP, TIP and NIP isoforms

Evans

Groszmann

2021

Preprint

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Aquaporins (AQPs) are involved in a variety of vital plant physiological processes, including water relations, development, stress responses and photosynthesis. Nicotiana tabacum (tobacco) has 84 AQP genes due to its recent hybridisation and allotetraploid genome. We functionally characterised a diverse subset of tobacco AQPs spanning the 3 largest AQP subfamilies, selecting nine isoforms from the PIPs, TIPs, and NIPs. Using high-throughput yeast-based functional assays, we determined AQP permeability to water, hydrogen peroxide (H2O2), boric acid (BA) and urea. For each AQP, subcellular localisations in planta were established using GFP translational fusions. From 3D protein homology modelling, we found that the monomeric pore shape/size, selectivity filter region and NPA motifs is insufficient to comprehensively predict their transport capabilities. PIPs had the narrowest pore diameter and were permeable to water, H2O2 and BA. The pore in TIPs was wider and more cylindrical in shape than for the PIPs. TIP1;1s was permeable to all four substrates tested, and is highly expressed in leaves and flowers, suggesting it functions in multiple roles. By contrast, NIP5;1t, with a larger pore size than the NtTIPs, is only expressed in young flowers and enhanced permeability only to BA. Its homolog in Arabidopsis (AtNIP5;1) has the same substrate specificity and functions as a boron channel.

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Section: Discussionmentioning

confidence: 99%

Exploring the Plant Aquaporin Solute Transport Network: Functional characterisation ofNicotiana tabacumPIP, TIP and NIP isoforms

Evans

Groszmann

2021

Preprint

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“…Plasma membrane intrinsic proteins, located on the cytomembrane, are highly selective for transporting matrix, and plays an important role in maintaining cell water balance under various adversities [42]. Studies have shown that plants resist abiotic stress by regulating the density and activity of PIPs at cytomembrane [39,43,44,45]. Plants mainly regulate their response to stress through the expression or inhibition of PIPs genes of aquaporin family (Table 1).…”

Section: Discussionmentioning

confidence: 99%

“…Unlike other plants, in Ligustrum × vicaryi, the number of aquaporin gene in PIPs subfamily was nearly 2 times higher than that of TIPs subfamily and NIPs subfamily, while it was similar to that of TIPs subfamily and NIPs subfamily in other plants. PIPs located on the cytomembrane was highly selective to transport matrix, and was critical for maintaining the water balance of cells in plants [39]. Thus, it was speculated that the Ligustrum × vicaryi PIPs subfamily (LvPIPs) may play a major role in maintaining its own water balance of cells.…”

Section: Overview Of Stress Related-genes Of Aquaporin Gene Familymentioning

confidence: 99%

Identification of Aquaporin Gene Family in Response to Natural Cold Stress in Ligustrum × Vicaryi Rehd.

Dong

Niu

Zhou

et al. 2021

Preprint

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Background: Maintaining water balance in various adversities is a difficult and critical challenge for plants. Studies have shown that aquaporins located on cytomembrane play an important role in maintaining water homeostasis under various environmental stresses. Some studies have shown that aquaporins are involved in the tolerance mechanism of plant cells under cold stress, and the aquaporin gene family is closely related to the cold resistance of plants. Ligustrum × vicaryi Rehd. plays a significant role in urban landscaping with poor cold resistance at the seedling stage and early planting stage. Screening the target aquaporin genes of Ligustrum × vicaryi related to cold resistance during natural cold stress will provide a scientific theoretical basis for cold resistance breeding of Ligustrum × vicaryi.Results: In this study, the genome-wide identification of the aquaporin gene family was performed at four different overwintering periods in September, November, January and April, and finally 58 candidate Ligustrum × vicaryi aquaporin (LvAQP) genes were identified. The phylogenetic analysis revealed that four subfamilies of the LvAQP gene family: 32 PIPs, 11 TIPs, 11 NIPs, and 4 SIPs, among which there were more genes in the PIPs subfamily than that in other plants. The key LvAQP genes were found through analyzing aquaporin genes related to cold stress in other plants and LvAQP genes expression profiles. The up-regulated key LvAQP genes were Cluster-9981.114831, Cluster-9981.104986, and Cluster-9981.120365, and the down-regulated key LvAQP genes were Cluster-9981.112839, Cluster-9981.109034, Cluster-9981.89369, Cluster-9981.110451, Cluster-9981.107281, Cluster-9981.112777, Cluster-9981.112789, Cluster-9981.122691 and Cluster-9981.88037. These genes play a key role related to cold tolerance in the nature low temperature growth stage of Ligustrum × vicaryi. Conclusions: This study systematically identified the AQP gene family in Ligustrum × vicaryi and screened for 20 differential expression LvAQP genes related to cold stress, among which 11 genes belonged to PIPs subfamily. The results of this research will lay the foundation for further biological function verification of cold resistance-related aquaporin candidate genes in Ligustrum × vicaryi, especially PIPs subfamily, and provide theoretical basis and technical support for improving seedling quality and breeding.

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“…Unlike other plants, in Ligustrum × vicaryi, the number of aquaporin genes in the PIPs subfamily was nearly two times higher than that of TIPs subfamily and NIPs subfamily, while it was similar to that of the TIPs and NIPs subfamilies in other plants. PIPs located on the plasma membrane were highly selective to the transport matrix, and they are critical for maintaining the water balance of cells in plants [39]. Thus, it isspeculated that the Ligustrum × vicaryi PIPs subfamily (LvPIPs) may play a major role in maintaining its own water balance of cells.…”

Section: Phylogenetic Analysis Of Lvaqp Gene Familymentioning

confidence: 99%

Identification of Aquaporin Gene Family in Response to Natural Cold Stress in Ligustrum × vicaryi Rehd.

Dong

Niu

Qian

et al. 2022

Forests

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Plants are susceptible to a variety of abiotic stresses during the growing period, among which low temperature is one of the more frequent stress factors. Maintaining water balance under cold stress is a difficult and critical challenge for plants. Studies have shown that aquaporins located on the cytomembrane play an important role in controlling water homeostasis under cold stress, and are involved in the tolerance mechanism of plant cells to cold stress. In addition, the aquaporin gene family is closely related to the cold resistance of plants. As a major greening tree species in urban landscaping, Ligustrum× vicaryi Rehd. is more likely to be harmed by low temperature after a harsh winter and a spring with fluctuating temperatures. Screening the target aquaporin genes of Ligustrum × vicaryi responding to cold resistance under natural cold stress will provide a scientific theoretical basis for cold resistance breeding of Ligustrum × vicaryi. In this study, the genome-wide identification of the aquaporin gene family was performed at four different overwintering periods in September, November, January and April, and finally, 58 candidate Ligustrum × vicaryi aquaporin (LvAQP) genes were identified. The phylogenetic analysis revealed four subfamilies of the LvAQP gene family: 32 PIPs, 11 TIPs, 11 NIPs and 4 SIPs. The number of genes in PIPs subfamily was more than that in other plants. Through the analysis of aquaporin genes related to cold stress in other plants and LvAQP gene expression patterns identified 20 LvAQP genes in response to cold stress, and most of them belonged to the PIPs subfamily. The significantly upregulated LvAQP gene was Cluster-9981.114831, and the significantly downregulated LvAQP genes were Cluster-9981.112839, Cluster-9981.107281, and Cluster-9981.112777. These genes might play a key role in responding to cold tolerance in the natural low-temperature growth stage of Ligustrum × vicaryi.

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Molecular insights into the plasma membrane intrinsic proteins roles for abiotic stress and metalloids tolerance and transport in plants

Cited by 30 publications

References 84 publications

Exploring the Plant Aquaporin Solute Transport Network: Functional characterisation ofNicotiana tabacumPIP, TIP and NIP isoforms

Exploring the Plant Aquaporin Solute Transport Network: Functional characterisation ofNicotiana tabacumPIP, TIP and NIP isoforms

Identification of Aquaporin Gene Family in Response to Natural Cold Stress in Ligustrum × Vicaryi Rehd.

Identification of Aquaporin Gene Family in Response to Natural Cold Stress in Ligustrum × vicaryi Rehd.

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