Molecular Insights into Vesicle Tethering at the Golgi by the Conserved Oligomeric Golgi (COG) Complex and the Golgin TATA Element Modulatory Factor (TMF)

Miller, Victoria J.; Sharma, Prateek; Kudlyk, Tetyana; Frost, Laura S.; Rofe, Adam P.; Watson, Irene J.; Duden, Rainer; Lowe, Martin; Lupashin, Vladimir; Ungár, Dániel

doi:10.1074/jbc.m112.426767

Cited by 71 publications

(104 citation statements)

References 67 publications

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“…TMF was previously shown to be involved in vesicle tethering by bringing vesicle and target membranes into close apposition before fusion (14). Using TEM analysis on colon sections from WT and TMF −/− mice, we found that mucus-packed granules in goblet cells of TMF −/− mice do not fuse before mucus secretion, as is seen in WT mice, but, rather, are discharged of their cargo late in the secretion stage.…”

Section: Discussionmentioning

confidence: 92%

“…Tata Element Modulatory Factor (TMF/ARA160; TMF) is a Golgi-associated protein that is apically expressed in colonic enterocytes and goblet cells and has been shown to be involved in vesicle tethering to target membranes (13,14). We recently showed that KO mice lacking TMF/ARA160 (TMF −/− ) have a thicker, more robust colonic mucus layer, which significantly reduces adherent bacterial load and protects the animals from experimental acute colitis (13).…”

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confidence: 99%

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Reprogrammed and transmissible intestinal microbiota confer diminished susceptibility to induced colitis in TMF ^−/− mice

Bel

Elkis

Elifantz

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Significance Our data demonstrate that a knockout of a single gene (tmf1) leads to the beneficial reprogramming of the gut resident microbiota. This reprogramming results in a diminished susceptibility of the genetically modified animals to induced colitis. Notably, the reprogrammed bacterial profile is transmissible, thereby conferring altered microbiome and reduced susceptibility to induced colitis in wild-type mice, when cohoused. Our findings open previously unreported avenues for unraveling regulatory factors that affect the gut homeostasis and mammalian sensitivity to the onset of inflammatory bowel diseases.

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Section: Discussionmentioning

confidence: 92%

mentioning

confidence: 99%

Reprogrammed and transmissible intestinal microbiota confer diminished susceptibility to induced colitis in TMF ^−/− mice

Bel

Elkis

Elifantz

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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“…In yeast, the COG complex coordinates retrograde Golgi traffic and interacts with RAB, SNARE, and golgin proteins (reviewed in (88,91,92)). In plants, homologues of the COG complex have been predicted on the basis of sequence similarly (93), but there is little experimental evidence for the function and location of this complex in A. thaliana.…”

Section: The Yfp-got1 Enrichment Is Consistent With Previously Publismentioning

confidence: 99%

Identification of Regulatory and Cargo Proteins of Endosomal and Secretory Pathways in Arabidopsis thaliana by Proteomic Dissection*

Heard

Sklenář

Tomé

et al. 2015

Molecular & Cellular Proteomics

105

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The cell's endomembranes comprise an intricate, highly dynamic and well-organized system. In plants, the proteins that regulate function of the various endomembrane compartments and their cargo remain largely unknown. Our aim was to dissect subcellular trafficking routes by enriching for partially overlapping subpopulations of endosomal proteomes associated with endomembrane markers. We selected RABD2a/ARA5, RABF2b/ARA7, RABF1/ARA6, and RABG3f as markers for combinations of the Golgi, trans-Golgi network (TGN), early endosomes (EE), secretory vesicles, late endosomes (LE), multivesicular bodies (MVB), and the tonoplast. As comparisons we used Golgi transport 1 (GOT1), which localizes to the Golgi, clathrin light chain 2 (CLC2) labeling clathrincoated vesicles and pits and the vesicle-associated membrane protein 711 (VAMP711) present at the tonoplast. We developed an easy-to-use method by refining published protocols based on affinity purification of fluorescent fusion constructs to these seven subcellular marker proteins in Arabidopsis thaliana seedlings. We present a total of 433 proteins, only five of which were shared among all enrichments, while many proteins were common between endomembrane compartments of the same trafficking route. Approximately half, 251 proteins, were assigned to one enrichment only. Our dataset contains known regulators of endosome functions including small GTPases, SNAREs, and tethering complexes. We identify known cargo proteins such as PIN3, PEN3, CESA, and the recently defined TPLATE complex. The subcellular localization of two GTPase regulators predicted from our enrichments was validated using live-cell imaging. This is the first proteomic dataset to discriminate between such highly overlapping endomembrane compartments in plants and can be used as a general proteomic resource to predict the localization of proteins and identify the components of regulatory complexes and provides a useful tool for the identification of new protein markers of the endomembrane system. Molecular & Cellular

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“…Tethering can be mediated through direct interaction with the coat or through factors, such as SNAREs and Rab GTPases (Cai et al, 2007). Various tethers that mediate intraGolgi trafficking, such as p115 (Uso1) (Guo et al, 2008), the trafficking protein particle II (TRAPPII) complex (Yamasaki et al, 2009) and the conserved oligomeric Golgi (COG) complex (Miller et al, 2013) have been shown to bind the COPI coat. By recognizing specific elements that confer identity to these coated vesicles, possibly in combinations that allow for coincidence detection, these tethers target distinct types of COPI-coated vesicle for fusion with specific cisternae within the Golgi (Malsam et al, 2005).…”

Section: Uncoating Of Copi-coated Vesiclesmentioning

confidence: 99%

Formation of COPI-coated vesicles at a glance

Arakel

Schwappach

2018

Journal of Cell Science

103

106

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Molecular Insights into Vesicle Tethering at the Golgi by the Conserved Oligomeric Golgi (COG) Complex and the Golgin TATA Element Modulatory Factor (TMF)

Cited by 71 publications

References 67 publications

Reprogrammed and transmissible intestinal microbiota confer diminished susceptibility to induced colitis in TMF ^−/− mice

Reprogrammed and transmissible intestinal microbiota confer diminished susceptibility to induced colitis in TMF ^−/− mice

Identification of Regulatory and Cargo Proteins of Endosomal and Secretory Pathways in Arabidopsis thaliana by Proteomic Dissection*

Formation of COPI-coated vesicles at a glance

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Molecular Insights into Vesicle Tethering at the Golgi by the Conserved Oligomeric Golgi (COG) Complex and the Golgin TATA Element Modulatory Factor (TMF)

Cited by 71 publications

References 67 publications

Reprogrammed and transmissible intestinal microbiota confer diminished susceptibility to induced colitis in TMF −/− mice

Reprogrammed and transmissible intestinal microbiota confer diminished susceptibility to induced colitis in TMF −/− mice

Identification of Regulatory and Cargo Proteins of Endosomal and Secretory Pathways in Arabidopsis thaliana by Proteomic Dissection*

Formation of COPI-coated vesicles at a glance

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Reprogrammed and transmissible intestinal microbiota confer diminished susceptibility to induced colitis in TMF ^−/− mice

Reprogrammed and transmissible intestinal microbiota confer diminished susceptibility to induced colitis in TMF ^−/− mice