Molecular insights underlying the adverse effects of bisphenol A on gonadal somatic cells' steroidogenic activity

Palak, Ewelina; Lebiedzińska, Weronika; Lupu, Oana; Pulawska, Kamila; Anisimowicz, Sławomir; Mieczkowska, Aleksandra N.; Sztachelska, Maria; Niklińska, Gabriella Nicole; Milewska, Gabriela; Łukasiewicz, Monika; Ponikwicka‐Tyszko, Donata; Huhtaniemi, Ilpo; Wołczyński, S

doi:10.1016/j.repbio.2023.100766

Cited by 2 publications

(2 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…BPA is a ubiquitous environmental contaminant in Europe, America, Asia, and Australia, with concentrations ranging from 10 to >100,000 µg/kg dry weight [36]. The compound may induce adverse reproductive disorders in both men and women, although the data available do not converge in a single direction [26]. It is a common view that in females, BPA interferes with ovarian follicle development and steroidogenesis [13,43].…”

Section: Discussionmentioning

confidence: 99%

“…Currently available studies are mostly based on the effect of BPA on male and female steroidogenesis following long-term exposure (e.g., for 48 h [26]), to the compound at micromolar concentrations that are higher than the concentrations found in the environment. Very few data are available on the short-term effect of BPA on male and female reproduction in the presence of gonadotropins [24].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Short-Term Exposure to Bisphenol A Does Not Impact Gonadal Cell Steroidogenesis In Vitro

Roy

Lazzaretti

Paradiso

et al. 2023

Cells

View full text Add to dashboard Cite

Bisphenol A (BPA) is a ubiquitous, synthetic chemical proven to induce reproductive disorders in both men and women. The available studies investigated the effects of BPA on male and female steroidogenesis following long-term exposure to the compound at relatively high environmental concentrations. However, the impact of short-term exposure to BPA on reproduction is poorly studied. We evaluated if 8 and 24 h exposure to 1 nM and 1 µM BPA perturbs luteinizing hormone/choriogonadotropin (LH/hCG)-mediated signalling in two steroidogenic cell models, i.e., the mouse tumour Leydig cell line mLTC1, and human primary granulosa lutein cells (hGLC). Cell signalling studies were performed using a homogeneous time-resolved fluorescence (HTRF) assay and Western blotting, while gene expression analysis was carried out using real-time PCR. Immunostainings and an immunoassay were used for intracellular protein expression and steroidogenesis analyses, respectively. The presence of BPA leads to no significant changes in gonadotropin-induced cAMP accumulation, alongside phosphorylation of downstream molecules, such as ERK1/2, CREB and p38 MAPK, in both the cell models. BPA did not impact STARD1, CYP11A1 and CYP19A1 gene expression in hGLC, nor Stard1 and Cyp17a1 expression in mLTC1 treated with LH/hCG. Additionally, the StAR protein expression was unchanged upon exposure to BPA. Progesterone and oestradiol levels in the culture medium, measured by hGLC, as well as the testosterone and progesterone levels in the culture medium, measured by mLTC1, did not change in the presence of BPA combined with LH/hCG. These data suggest that short-term exposure to environmental concentrations of BPA does not compromise the LH/hCG-induced steroidogenic potential of either human granulosa or mouse Leydig cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Short-Term Exposure to Bisphenol A Does Not Impact Gonadal Cell Steroidogenesis In Vitro

Roy

Lazzaretti

Paradiso

et al. 2023

Cells

View full text Add to dashboard Cite

show abstract

The effects of bisphenol A and its analogs on steroidogenesis in MA-10 Leydig cells and KGN granulosa cells

Iskandarani,

Romanelli,

Hales

et al. 2024

Biology of Reproduction

View full text Add to dashboard Cite

Bisphenols are a family of chemicals used in the manufacture of consumer products containing polycarbonate plastics and epoxy resins. Studies have shown that exposure to bisphenol A (BPA) may disrupt steroidogenesis and induce adverse effects on male and female reproduction, but little is known about BPA replacements. We determined the effects of six bisphenols on the steroidogenic function of MA-10 Leydig cells and KGN granulosa cells by measuring the levels of progesterone and estradiol produced by these cells as well as the expression of transcripts involved in steroid and cholesterol biosynthesis. MA-10 and KGN cells were exposed for 48 hours to one of six bisphenols (0.01–50 μM): BPA, bisphenol F, bisphenol S, bisphenol AF, bisphenol M, or bisphenol TMC under both basal and dibutyryl cAMP (Bu2cAMP)-stimulated conditions. In MA-10 cells, most bisphenols increased the Bu2cAMP-stimulated production of progesterone. In KGN cells, there was a general decrease in progesterone production, while estradiol levels were increased following exposure to many bisphenols. qRT-PCR analyses revealed that all six bisphenols (≥1 μM) upregulated the expression of STAR, a cholesterol transporter, in both cell lines after stimulation. Key transcripts directly involved in steroid and cholesterol biosynthesis were significantly altered in a cell line, chemical, and concentration-dependent manner. The expression of upstream genes was also differentially affected. Thus, BPA and five of its analogs can disrupt steroid production in two steroidogenic cell lines and alter the levels of transcripts involved in this process. Importantly, BPA replacements do not appear to have fewer effects than BPA.

show abstract

Molecular insights underlying the adverse effects of bisphenol A on gonadal somatic cells' steroidogenic activity

Cited by 2 publications

References 60 publications

Short-Term Exposure to Bisphenol A Does Not Impact Gonadal Cell Steroidogenesis In Vitro

Short-Term Exposure to Bisphenol A Does Not Impact Gonadal Cell Steroidogenesis In Vitro

The effects of bisphenol A and its analogs on steroidogenesis in MA-10 Leydig cells and KGN granulosa cells

Contact Info

Product

Resources

About