Molecular Interaction Between Cationic Polymer Polyethyleneimine and Rose Bengal Dye: A Spectroscopic Study

Bayraktutan, Tuğba

doi:10.18596/jotcsa.504528

Cited by 7 publications

(4 citation statements)

References 28 publications

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“…PEI is a polymer with a high concentration of amine groups in its structure. The amine groups provided a good environment for the formation of a CA-PEI complex, as reported in the literature (Bayraktutan, 2019). The redshift spectrum of approximately 52 nm observed in our experiment could be described by the complex formation between PEI and CA (see Figure S4, Supplementary Materials).…”

Section: Ca-pei Complexation Using the Dpad Approachsupporting

confidence: 84%

Development of distance paper-based devices and electrochemical sensors for food and cosmetic safety assessment

Katelakha

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This study aimed to integrate analytical techniques for supporting the safety assessment in food and cosmetics. The two analytical methods were proposed, including i) the development of the paper-based device for the screening detection of lead (Pb) in food matrices, ii) development of the disposable screen-printed electrode for the detection of non-permitted color in halal products. For the first approach, the assay principle was�based on competitive binding between carminic acid (CA) and polyethyleneimine (PEI) to Pb in food samples. The reduction of the PEI-CA color distance is proportional to the concentration of Pb. To categorize food which higher or lower the cut-off of the Pb concentration (2 ?g?mL?1), the standard addition technique combined with the concept of a drawing PAD was integrated using a series of standard addition (8.0, 9.0, and 10.0 ?g?mL?1). The results obtained from the dPAD are in accordance with the concentration measured by the atomic absorption spectroscopy (AAS). The results from the second approach demonstrated that carminic acid and carmoisine were simultaneously assayed under the acidic condition (100 mM sodium citrate buffer, pH 3.0). The electrode was scanned with differential pulse voltammetry (DPV) from ?1.5 V to +1.5 V, and by using a bare unmodified electrode, the detection limit was obtained at 33.68 ?M. The method was obtained with acceptable precision with 2.78? 9.52 %CVs. We demonstrate the proof-of-concept for the detection of carminic acid in lipsticks. The recovery of > 80% was comparable to those obtained by ultra-high performance liquid chromatography (ultra-HPLC). The recovery study of carminic acid in the beverage and raw material (cosmetic grade) ranged from 101.9% ? 123.6% and 93.2% ? 109.0%, respectively. Furthermore, to improve the sensitivity of this technique sufficient to detect the CA fortified in actual food samples, the electrode surface was modified with AuNP/PEI composite. Interestingly, the sensitivity was dramatically 24-times improved comparing to the bare electrode. A linear range of 10 100 ?M (R2�= 0.986) was achieved by using DPV and setting the potential at 0.297 V. In conclusion, an electrochemical method based on a screen-printed electrode modified with AuNP/PEI composite was a promising tool for sensitive detection of carminic acid for assisting the safety monitoring in halal products.

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Section: Ca-pei Complexation Using the Dpad Approachsupporting

confidence: 84%

Development of distance paper-based devices and electrochemical sensors for food and cosmetic safety assessment

Katelakha

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“…28 In contrast, under polycation concentration conditions where [Θ] T /[RB 2− ] ≥ 0.5, both the absorbance and fluorescence emission bands of the dye are red-shifted by about 14 nm, with a hyperchromic and narrowing effect of the absorbance band and a sharp increase in the Φ F value of RB 2− . This behavior has been extensively reported for dye binding to proteins 29 and polyelectrolytes, 26,30 due to the association of a single dye molecule per binding site, inducing the loss of the dye aggregation on the polycation by the presence of more available binding sites as the polycation concentration increases. 26 Under this condition, the ground-and excitedstate spectroscopic properties of the dye resemble those found for monomeric RB 2− in organic solvents sensing a less-polar local environment, 31 supporting the formation of a 1:1 adduct between a free dye molecule (RB 2− free ) and a free binding site (Θ) in the polycation as described by the following pseudophase equilibrium with a dissociation constant K d given by eq 1.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“On-Demand” Antimicrobial Photodynamic Activity through Supramolecular Photosensitizers Built with Rose Bengal and (p-Vinylbenzyl)triethylammomium Polycation Derivatives

et al. 2022

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The antimicrobial photodynamic activity (aPDA) in fungal and bacterial strains of supramolecular adducts formed between the anionic photosensitizer (PS) Rose Bengal (RB 2− ) and aromatic polycations derived from (p-vinylbenzyl)triethylammonium chloride was evaluated. Stable supramolecular adducts with dissociation constants K d ≈ 5 μM showed photosensitizing properties suitable for generating singlet oxygen (Φ Δ = 0.5 ± 0.1) with the added advantage of improving the photostability of the xanthenic dye. However, the aPDA of both free and supramolecular RB 2− was highly dependent on the type of microorganism treated, indicating the importance of specific interactions between the different cell wall structures of the microbe and the PSs. Indeed, in the case of Gram-positive Staphylococcus aureus, the aPDA of molecular and supramolecular PSs was highly effective. Instead, in the case of Gram-negative Escherichia coli, only the RB 2− :polycation adducts showed aPDA, while RB 2− alone was inefficient, but in the case of Candida tropicalis, the opposite behavior was observed. Therefore, the present results indicate the potential of supramolecular chemistry to obtain aPDA àla carte depending on the target microbe and the PS properties.

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“…PEI is a polymer with a high concentration of amine groups in its structure. The amine groups provided a good environment for the formation of a CA–PEI complex, as reported in the literature [ 48 ]. The redshift spectrum of approximately 52 nm observed in our experiment could be described by the complex formation between PEI and CA (see Figure S4, Supplementary Materials ).…”

Section: Discussionmentioning

confidence: 98%

A Simple Distance Paper-Based Analytical Device for the Screening of Lead in Food Matrices

et al. 2021

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A simple and rapid distance paper-based analytical device (dPAD) for the detection of lead (Pb) in foods is proposed herein. The assay principle is based on competitive binding between carminic acid (CA) and polyethyleneimine (PEI) to Pb in a food sample. The paper channels were pre-immobilized with PEI, before reacting with a mixture of the sample and CA. Pb can strongly bind to the CA; hence, the length of the red color deposition on the flow channel decreased as a lower amount of free CA bound to PEI. The dPAD exhibited good linear correlation, with ranges of 5–100 µg·mL−1 (R2 = 0.974) of Pb. Although, the limit of detection (LOD) of this platform was rather high, at 12.3 µg·mL−1, a series of standard additions (8.0, 9.0, and 10.0 µg·mL−1) can be used to interpret the cutoff of Pb concentrations at higher or lower than 2 µg·mL−1. The presence of common metal ions such as calcium, magnesium, nickel, and zinc did not interfere with the color distance readout. The validity of the developed dPAD was demonstrated by its applicability to screen the contamination of Pb in century egg samples. The results obtained from the dPAD are in accordance with the concentration measured by atomic absorption spectroscopy (AAS) (n = 9). In conclusion, this proposed dPAD, combined with the standard addition method, could be applied for screening Pb contamination in food matrices. This platform is, therefore, potentially applicable for field measurements of Pb in developing countries, because it is cheap and rapid, and it requires no significant laborious instruments.

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Molecular Interaction Between Cationic Polymer Polyethyleneimine and Rose Bengal Dye: A Spectroscopic Study

Cited by 7 publications

References 28 publications

Development of distance paper-based devices and electrochemical sensors for food and cosmetic safety assessment

Development of distance paper-based devices and electrochemical sensors for food and cosmetic safety assessment

“On-Demand” Antimicrobial Photodynamic Activity through Supramolecular Photosensitizers Built with Rose Bengal and (p-Vinylbenzyl)triethylammomium Polycation Derivatives

A Simple Distance Paper-Based Analytical Device for the Screening of Lead in Food Matrices

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