Molecular Interaction Kinetics and Mechanism Study of Phytohormones and Plant Protein with Fluorescence and Synchronous Fluorescence Techniques

Abstract

Cytokinins (CTKs) are widely used in agriculture to enhance crop production. To understand the role of CTKs in plant growth is vital, however, molecular mechanisms underlying CTKs action particularly their binding kinetics with proteins are not clear. The study of these interactions can obtain important information on how these signal molecules regulate cellular processes in targeted cells locally or transport to other functional parts of plants. In this study, the interactions between two CTKs and soy protein were investigated. The binding constants determined at 30 o C were K KT-soy = 1.5 3 10 3 M À1 and K IP-soy = 0.67 3 10 2 M À1 and the binding stoichiometry was n KT-soy = 0.530 and n IP-soy = 0.5165 respectively. Moreover, the CTK-protein interaction is found to be a static quenching process via an energy transfer mechanism. The adduct formed through electrostatic interactions under the plant acidity conditions was thus transported to the action parts of the plant to control growth process.

One-Step Co-Electrodeposition of Copper Nanoparticles-Chitosan Film-Carbon Nanoparticles-Multiwalled Carbon Nanotubes Composite for Electroanalysis of Indole-3-Acetic Acid and Salicylic Acid

“…Therefore, it is necessary to establish a method for the determination of IAA and SA with high selectivity and sensitivity. The current detection methods include liquid chromatography-mass spectrometry [5,6], molecular imprinting method [7,8], capillary electrophoresis [9], fluorescence spectroscopy [10] and electrochemical method [11]. Among them, electrochemical method is favored for its advantages of simple operation, high sensitivity, and fast analysis compared with other methods.…”

“…Figure 10. LSV (A) curve of CuNPs-CSF-CNPs-MWCNTs/GCE in 0.1 M PBS (pH = 7.0) containing different concentrations of IAA (0.01, 0.1, 5, 10, 20, 30, 40, 50 μM) and SA(4,6,10,14,18,22,26,30 …”