Molecular investigation and diagnosis of three genetic diseases in Arabian horses from Middle East and North American populations

Khanshour, Anas M.; Juras, Rytis; Stelly, L.; Cothran, E. Gus

doi:10.1016/j.jevs.2013.03.102

Cited by 3 publications

(3 citation statements)

References 6 publications

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“…On the other hand, Ayad et al 17 analysed 177 samples obtained from three horse breeds belonging to the Middle East and North Africa regions and found none of them had this lethal condition. This genetic defect, however, has not been detected in the Arabian horse populations of Syria, 18 Poland 10 and Turkey, 11 suggesting that this deadly mutation has still not been introduced in some populations.…”

Section: Discussionmentioning

confidence: 95%

Evidence for origin of lavender foal syndrome among Egyptian Arabian horses in Egypt

Ela

Araby

Saleh

et al. 2022

Equine Veterinary Journal

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Background Lavender foal syndrome (LFS) is a fatal hereditary condition that is inherited in an autosomal recessive pattern. This detrimental mutation is more common in Arabian foals of Egyptian origin than foals from other bloodlines. Heterozygous horses are carriers of the LFS trait and appear normal, while recessive homozygous foals died shortly after birth due to serious complications. In Egypt, in 2014, an Egyptian foal died after manifestations of neurological signs and abnormal coat colour as LFS signs. Therefore, it is important to identify LFS carriers in the population of Arabian horses in Egypt and to encourage improvement of the Arabian horse industry in Egypt by constructing a breeding system based on genetic background in order to avoid mating between carriers and reduce financial losses from deaths of affected foals. Objectives To establish a PCR‐based test for detecting the MYO5A gene mutation causing LFS in the registered Arabian horse population in Egypt prior to breeding. Study design Cross sectional survey (n = 170) plus targeted sampling (n = 30). Methods A total of 200 samples were collected from an Arabian farm in Egypt and some of them were traced for LFS based on the farm records. The LFS genotypes were identified using the PCR‐RFLP technique, fragment analysis followed by sequence analysis. Results The overall mutated allele and genotype frequencies (N/L) were 0.08 and 16%, respectively. Conclusion The observed frequency of heterozygotes suggests foals affected with LFS will be produced among Arabian horses in Egypt. Therefore, screening of the entire population for this mutation should be undertaken in the breeding program.

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Section: Discussionmentioning

confidence: 95%

Evidence for origin of lavender foal syndrome among Egyptian Arabian horses in Egypt

Ela

Araby

Saleh

et al. 2022

Equine Veterinary Journal

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“…In addition to electrophoresis, sequencing analysis could be performed to identify the genotypes of a population in terms of the presence/absence of SCID mutant alleles, as in these studies [1,25]. The studies that investigated SCID in Arabian horses in the literature were summarized in Table. The SCID mutant allele was not observed in Arabian horse populations in Poland [18], Romania [16], Slovenia [17], Latvia [27], Iran [15], Syria [14], Ukraine [13], Egypt [1,28], or Turkey [19]. Conversely, the SCID mutant allele was identified in Arabian horse populations in the following prevalence: 8.4% in the USA [8], 2.8% in England [9], 1.5% in Brazil [10], 6.4% (in the year 2004) and 3.4% (in the year 2009) in South Africa [12], 5.6% in Morocco [11], 1.6% in Iran [26], and 0.4% in Poland [25].…”

Section: Discussionmentioning

confidence: 99%

“…Among Arabian horse populations tested using molecular methods, the SCID mutant gene was detected in the USA [8], the UK [9], Brazil [10], Morocco [11], Egypt [1], and South Africa [12], but could not be detected in Ukraine [13], Syria [14], Iran [15], Romania [16], Slovenia [17], Poland [18], or Turkey [19]. There was only one study on SCID in Turkish Arabian horses.…”

Section: Introductionmentioning

confidence: 99%

Investigation of the SCID mutant allele in Turkish Arabian horses reared in some private farms of the Eskişehir region in Turkey

Doğan¹,

Kaya²

2020

Turk J Vet Anim Sci

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There are several known hereditary diseases in Arabian horses. Many studies have been conducted on molecular diagnostic methods of the mutations that cause these hereditary defects in horses. A five-base deletion in the short arm (ECA9p12) of the equine chromosome 9, starting with codon 9480, which occurs in the catalytic subunit gene of the DNA-dependent protein kinase (DNA-PKcs) results in severe combined immunodeficiency (SCID) hereditary disorder. SCID is an autosomal recessive disorder wherein the immune system of the affected foal is very weak. Purebred Turkish Arabian horses reared in the Eskişehir region have contributed greatly to Turkish Arabian horse breeding and racing. The aim of this study was to detect the SCID mutant allele by using DNA sequencing analysis in Turkish Arabian horses, raised in nine different private farms in Eskişehir. It was determined that none of the horses evaluated in this study carried the SCID mutant allele.

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Determination of Sublethal Mutation Causing Lavender Foal Syndrome in Arabian Horses From Croatia

Efendić

Mačešić

Samardžija

et al. 2018

Journal of Equine Veterinary Science

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Molecular investigation and diagnosis of three genetic diseases in Arabian horses from Middle East and North American populations

Cited by 3 publications

References 6 publications

Evidence for origin of lavender foal syndrome among Egyptian Arabian horses in Egypt

Evidence for origin of lavender foal syndrome among Egyptian Arabian horses in Egypt

Investigation of the SCID mutant allele in Turkish Arabian horses reared in some private farms of the Eskişehir region in Turkey

Determination of Sublethal Mutation Causing Lavender Foal Syndrome in Arabian Horses From Croatia

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