Molecular Investigation of Some Bacteria (Coxi̇ella Burneti̇i̇, Mycoplasma Haemocani̇s, Candi̇datus Mycoplasma Haematoparvum, Wolbachi̇a) in Rhipicephalus Sanguineus Ticks in Siirt Province, Turkey

Abstract

Ticks harbor the largest diversity of microorganisms, ranging from viruses, prokaryotes, and eukaryotes. Rhipicephalus sanguineus ticks are the most common ticks worldwide. Although dogs are the main host of this tick species, it has been reported that it also infests humans in various parts of the world. This study aimed to examine some bacteria (Coxiella burnetii, Mycoplasma haemocanis, Candidatus Mycoplasma haematoparvum, Wolbachia) in Rhipicephalus sanguineus ticks sampled from dogs. In this study, 350 tick samples collected from 85 dogs in Siirt province were determined to be Rhipicephalus sanguineus ticks. Coxiella DNA was detected in 3 (0.85%) out of 350 ticks using Nested PCR (687 base pairs). None of the samples were found to contain Mycoplasma haemocanis, Candidatus Mycoplasma haematoparvum, and Wolbachia DNA. A partial sequence of the IS1111 gene region was registered in GenBank with OM472143 accession numbers. Considering the zoonotic nature of the Q disease, it is very important for dog owners and related institutions to periodically spray animals against ticks, and to take any other necessary precautions. More samples are needed to determine the Mhc, CMhp, and Wolbachia prevalence.

Molecular detection and identification of hemotropic Mycoplasma species in dogs and their ectoparasites in Iran

“…ticks are found more commonly 33 hypothesizing that this tick may be a possible vector for the transmission of canine hemoplasma species. In contrast, none of 350 individual R. sanguineus ticks collected from dogs in a study in Turkey where brown dog tick is prevalent 34 ; and none of the tick specimens including 204 fully engorged nymphs removed from dogs, and 2100 nymphs and 85 adults collected from the grounds of the same shelter where both pathogens were present in examined dogs suggest that R. sanguineus s.l. may not be a suitable vector for canine hemoplasma species in the eld conditions 22 .…”