Molecular investigation on the presence of canine parvovirus in Egypt

Elbaz, Elzahara; El‐Tholoth, Mohamed; Elfadl, Eman A. Abo; Mosad, Samah M.

doi:10.1016/j.cimid.2020.101576

Cited by 8 publications

(12 citation statements)

References 24 publications

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“…Regarding breed disposition, the German shepherd breed was found to be the most predisposed breed in this study followed by Pit bull, Golden Pikinwa, Boxer and Griffon. This came in agreement with previous studies which reported that Pit bull and German shepherd are more susceptible to be infected with CPV-2 than other dog breeds (AL-Hosary, 2018;Elbaz et al, 2021). The immunochromatographic assay is a simple, inexpensive, and rapid CPV diagnostic method available in veterinary clinic practice.…”

Section: Discussionsupporting

confidence: 92%

“…Frontiers in Microbiology 08 frontiersin.org CPV-2, as it was able to detect 272/320 (85%) of infected cases based on the amplification of a highly conserved region of the VP2 gene, which is similar to previous research at the national level (Elbaz et al, 2021). Previous study revealed that negative IC test results do not exclude CPV-2 infection, but positive IC test result always indicates CPV-2 infection (Kantere et al, 2015).…”

Section: Discussionsupporting

confidence: 82%

“…While (Awad et al, 2018) reported Parvovirus isolates which were 100% related to Portugalian isolates. Serotypes CPV-2b/2c were reported by Elbaz et al (2021) and CPV-2a and CPV-2b were also reported in previous Egyptian studies (AL-Hosary, 2018;Soliman et al, 2018;El-Neshwy et al, 2019). At present (Zaghawa and Abualkhier, 2019) recorded that the three variants are circulating in dog population in Egypt.…”

Section: Discussionsupporting

confidence: 77%

“…This amino acid substitution tendency of (N426E) (CPV-2c carrying a Glu) on residue 426 of VP2 was first detected in 2000 in Italy (Jantafong et al, 2022). This mutation has been also emphasized by Elbaz et al (2021) who confirmed that the main residue to distinguish between the three strains was residue 426. Changing an asparagine (N) to glutamine (E) in a protein can have significant ramifications for its arrangement, operation, and interactions.…”

Section: Discussionmentioning

confidence: 71%

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Molecular typing of canine parvovirus type 2 by VP2 gene sequencing and restriction fragment length polymorphism in affected dogs from Egypt

Magouz,

El-Kon,

Raya-Álvarez

et al. 2023

Front. Microbiol.

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IntroductionCanine parvovirus-2 (CPV-2) is one of the most common infectious diseases in dogs characterized by severe gastroenteritis, vomiting, and bloody diarrhea. Little information is available about this topic in Egypt, particularly in the Delta region. This study reports the prevalence and molecular analysis of CPV-2 variants collected from El-Gharbia and Kafrelsheikh governorates in the Delta of Egypt.MethodsIn this study, 320 rectal swabs were collected from infected domestic dogs from two districts in delta Egypt. The samples were investigated by rapid immunochromatographic test and polymerase chain reaction for detection the prevalence of CPV-2 variants. The genetic characterization was performed using restriction fragment length polymorphism (RFLP) analysis and partial VP2 gene sequence.Results and discussionThe viral antigen was detected in (264/320, 82.5%) of samples by IC test, while PCR was found more sensitive by detecting (272/320, 85%) positive samples. The RFLP technique using MboII restriction enzyme was successfully used for the differentiation of CPV-2c antigenic variants from CPV-2a/2b strains. Interestingly, the molecular and phylogenetic analysis revealed that both CPV-2a and CPV-2c are circulating in the study area. Deduced amino acid sequence analysis showed changes at residue (N426E) and residue (T440A).: Our results indicated that CPV-2 is prevalent among dogs in Egypt, and therefore further molecular and epidemiological studies of CPV-2 are warranted.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 82%

Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 71%

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Molecular typing of canine parvovirus type 2 by VP2 gene sequencing and restriction fragment length polymorphism in affected dogs from Egypt

Magouz,

El-Kon,

Raya-Álvarez

et al. 2023

Front. Microbiol.

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“…The CPV was first found in Egyptian military police dogs in 1982 and manifested clinically and pathologically. FPV has been identified; the circulating genotypes in Egypt were PV-2a and CPV-2b (El-Neshwy et al, 2019;Zaher et al, 2020;Elbaz et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Feline Pan Leukopenia Molecular Detection and Viral Phylogeny in Egypt

Khattab¹,

Yanni²,

Abdelmegeed³

et al. 2023

AAVS

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Feline panleukopenia virus (FPV) causes severe disease in cats, as well as in a variety of wild carnivores including many endangered animals. Regular appearance of the virus increases the importance of evaluating the percentage of identity in relation to vaccine commercially used, detecting sequence changes related to virus virulence or tropism. In this study Fifty-seven fecal samples were collected from suspected cases of cats. 19 fecal samples were positive using Rapid FLV Ag test kits kit (VDRG FLV/FIV Ab rapid kit). Fifty-seven blood samples of the same animals were subjected for hematological examination to investigate the severity of leukopenia. Thirteen out of nineteen samples were positive with a polymerase chain reaction assay (PCR) targeting VP2 gene. Three positive fecal samples in animal showing sever and moderate leukopenia were successfully sequenced Partially. Sequence analysis results showed no variation in their sequence across all isolates when compared to the published FPV genome, which could imply that FPV appears to be in genomic stasis as compared to other Parvoviruses. Genomic stasis appears among the three Egyptian samples in this study under the GenBank accession number OP594200, OP574201, and OP574202, in addition to the high similarity of the isolates the vaccine strains of EU498680 and EU498681.No amino acid substitution was detected.

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