Molecular‐level pursuit of yeast mitosis by time‐ and space‐resolved Raman spectroscopy

Abstract

The process of eukaryotic cell division has been studied at the molecular level by Raman spectroscopy. Time-and space-resolved Raman spectra of a living fission yeast cell were successfully recorded with a 100 s time resolution and a 250 nm spatial resolution by using a confocal multi-channel Raman microspectrometer. Raman spectra characteristic of the nucleus, mitochondrion and septum were identified and time-dependent changes of major molecular components during the cell cycle were elucidated.

Raman spectroscopic signature of life in a living yeast cell

“…As shown previously, 9 . This coincidence is fully consistent with the fact that mitochondrion is an organelle that is highly rich in phospholipids.…”

“…We were able to measure the time-and space-resolved Raman spectra of organelles such as the nucleus, mitochondrion and septum of a dividing yeast cell. 9 It is now possible for us to obtain Raman spectroscopic signatures that are characteristic of a particular organelle Ł Correspondence to: Hiro-o Hamaguchi, Department of Chemistry, School of Science, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. E-mail: hhama@chem.s.u-tokyo.ac.jp at a particular cell stage of a living cell and hence to watch the growing and dying processes of the cell at the molecular level.…”

Chemotaxonomic Identification of Single Bacteria by Micro-Raman Spectroscopy: Application to Clean-Room-Relevant Biological Contaminations

“…Very recently, Maquelin et al (31) performed for the first time a clinical Fourier transform IR and near-IR-Raman study of bacterial contamination in blood cultures by using microcolonies obtained after 6 to 8 h of cultivation. Other papers have reported Raman and SERS investigations of single yeast cells, bacteria, or spores (1,10,21,(60)(61)(62). Various investigations of cell components of single bacteria or spores by means of Raman spectroscopy have also been reported (16,20,22,40,50,51).…”

Raman spectroscopic identification of single yeast cells

“…Xie and coworkers performed measurements on single yeast cells by means of Raman spectroscopy in combination with optical tweezers. 6,7 Further studies on Raman spectroscopic monitoring of yeast mitosis 8 5 From these Raman images, no dependence on the spatial position of measurement was observed, i.e. the bacterium shows spatial homogeneity.…”