Molecular Localization of the Transforming and Secretory Properties of PDGF A and PDGF B

Abstract

Human platelet-derived growth factor (PDGF) is a connective tissue cell mitogen comprised of two related chains encoded by distinct genes. The B chain is the homolog of the v-sis oncogene product. Properties that distinguish these ligands include greater transforming potency of the B chain and more efficient secretion of the A chain. By a strategy involving the generation of PDGF A and B chimeras, these properties were mapped to distinct domains of the respective molecules. Increased transforming efficiency segregated with the ability to activate both alpha and beta PDGF receptors. These findings genetically map PDGF B residues 105 to 144 as responsible for conformational alterations critical to beta PDGF receptor interaction and provide a mechanistic basis for the greater transforming potency of the PDGF B chain.

Insect prothoracicotropic hormone: a new member of the vertebrate growth factor superfamily

“…The receptor binding sites offl-NGF [29,30] and PDGF-BB [31] have been located in the peripheral, conformationally variable regions outside the common fold that we defined, though 6-- Fig. 2.…”

Suppression of glioblastoma tumorigenicity by the Kruppel-like transcription factor KLF6

“…The pCIneo-KLF6 expression construct was created by fusing a full-length KLF6 cDNA in the EcoRI and XbaI sites of the pCIneo vector. The c-sis/PDGF-B expression plasmid has previously been described (LaRochelle et al, 1990). The pBabepuro-v-src (Osada et al, 1999), pCEV29-H-Ras R12 (Chan et al, 1994), and pLTR2-EGFR (Di Fiore et al, 1987) plasmids have been described previously.…”

Multiple Growth Factor Induction of a Murine Early Response Gene That Complements a Lethal Defect in Yeast Ribosome Biogenesis

“…PDGF and the FGFs are known to be potent fibroblast mitogens (31,32). Similarly, hepatocyte and keratinocyte growth factors induce growth and proliferation of epithelial cells (33,34).…”