Molecular Manipulations for Enhancing Luminescent Bioreporters Performance in the Detection of Toxic Chemicals

Yagur‐Kroll, Sharon; Belkin, Shimshon

doi:10.1007/978-3-662-43619-6_4

Cited by 9 publications

(5 citation statements)

References 38 publications

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“…Express methods based on microbial wholecell bioreporters are the most promising among them for use in drug toxicology. Microbial whole-cell bioreporters are genetically modified micro-organisms that produce a quantifiable output in response to the presence of toxic chemicals or other stress factors (14).…”

mentioning

confidence: 99%

Synthesis of New ‘Hybrid’ Compounds Based on Benzofuroxans and Aminoalkylnaphthalimides

et al. 2015

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Pathogenic bacteria and fungi eventually develop resistance to existing drugs, and therefore, we need constant development of new drugs. The research is aimed at addressing fundamental scientific problems-the search for new biologically active compounds among several benzofuroxan-containing 'hybrid' products. N-substituted naphthalimides were chosen as a second pharmacophore. Benzofuroxanes biological effects were studied by means of bacterial lux-biosensors. Compounds IIIa, IVa, IIIc, and IVc displayed more expressed bacteriotoxic action in comparison with the initial substances Ia-c and represent a certain interest for using as antibacterial substances.

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mentioning

confidence: 99%

Synthesis of New ‘Hybrid’ Compounds Based on Benzofuroxans and Aminoalkylnaphthalimides

et al. 2015

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“…The genotype of chassis strains can profoundly affect the performance of the sensing pathways carried (Yagur-Kroll and Belkin, 2014). For example, Julien et al observed a 10-fold increase in sensitivity of nickel-iron WCB when E. coli TD2158 was used as a chassis instead of E. coli K12 (Cayron et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

A smartphone-based genetically recombinant whole-cell biosensor for highly sensitive monitoring of polychlorinated biphenyls (PCBs)

Luo,

Zhang,

Zhang

et al. 2024

Preprint

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Polychlorinated biphenyls (PCBs) are highly carcinogenic and persistent pollutants commonly found in ecosystems. Their complex congeners pose a huge challenge to instrumental analysis and ELISA methods, which prefer single and known targets. To overcome this limitation, here we developed anEscherichia coliwhole-cell biosensor (WCB) for simultaneously detecting multiple PCB congeners. In this sensor, PCBs were firstly converted into hydroxylated PCBs (OH-PCBs) bybphABdegradation circuits, which then serve as high-affinity targets of transcriptional factor HbpRCBP6-based sensing pathways for sensitive response through extensive chassis screening. The resulting biosensor BL21(DE3)/HbpRCBP6-bphABshows the lowest detection limits for 2-CBP (2-chlorobiphenyl) to date and can recognize various PCB homologues, including 3-CBP, 4-CBP, 2,3-diCBP and 2,2’-diCBP, with detection limits of 0.06-1 μM. Further investigation of the docking structure and binding energy reveal that HbpRCBP6has a stronger affinity for OH-PCBs than for PCBs, indicating that the conversion of PCB by BphAB enzymes is a key step to improve the sensitivity of WCB. Subsequently, we developed an immobilized hydrogel WCB and a smartphone-based detection procedure to facilitate real-time and user-friendly PCB detection. This study will not only advance the biomonitoring of PCB contaminants but also provide an innovative strategy for developing metabolic pathway-sensing proteins combined biosensor.Graphical Abstract

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“…Therefore, based on the traditional luminescent bacteria bioassay, two new detection methods are proposed. One involves using genetic engineering to transfer the structural genes responsible for luminescence to other bacteria and assemble them into recombinant luminescent bacteria, which can realize the detection of specific pollutants [27,28]. The other is to use magnetic nanomaterials and luminescent bacteria to assemble magnetic nanoparticles (MNPs)-based biosensors, which can be separated from the soil solution and tested under an external magnetic field, without centrifugation and other operations, to realize the detection of undisturbed soil [29].…”

Section: Introductionmentioning

confidence: 99%

Application of Luminescent Bacteria Bioassay in the Detection of Pollutants in Soil

et al. 2023

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The luminescent bacteria bioassay has been commonly used in the detection of environmental pollutants. Compared with traditional chemical and other biological detection methods, the luminescent bacteria bioassay has many demonstrated advantages such as a sensitive response, low cost, high efficiency, and environmental friendliness. The traditional luminescent bacteria bioassay has poor reproducibility and cannot achieve undisturbed soil testing, and the use of leach liquor also affects the results. This paper reviews the research progress and existing issues for the traditional luminescent bacteria bioassay used in the detection of soil pollutants. The luminescence mechanisms and detection principles of three commonly used luminescent bacteria, i.e., Vibrio fischeri, Photobacterium phosphoreum, and Vibrio qinghaiensis, are discussed and compared. In addition, two new luminescent bacteria bioassays are introduced to detect soil pollutants. One method is based on recombinant luminescent bacteria obtained with a gene-modification technique. This method can realize specific detection and enhance sensitivity, but it still cannot achieve undisturbed soil detection. The other method involves using magnetic nanoparticle (MNP)-based biosensors made from luminescent bacteria and MNPs. It can realize the accurate detection of the biological toxicity of the combined pollutants in soil without disturbing the soil’s integrity. This study shows that MNP-based biosensors have good application prospects in soil pollution detection, but the mechanism behind their utility still needs to be investigated to realize their popularization and application.

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Molecular Manipulations for Enhancing Luminescent Bioreporters Performance in the Detection of Toxic Chemicals

Cited by 9 publications

References 38 publications

Synthesis of New ‘Hybrid’ Compounds Based on Benzofuroxans and Aminoalkylnaphthalimides

Synthesis of New ‘Hybrid’ Compounds Based on Benzofuroxans and Aminoalkylnaphthalimides

A smartphone-based genetically recombinant whole-cell biosensor for highly sensitive monitoring of polychlorinated biphenyls (PCBs)

Application of Luminescent Bacteria Bioassay in the Detection of Pollutants in Soil

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