Molecular mapping of a new gene in wild barley conferring complete resistance to leaf rust (Puccinia hordei Otth)

Ivandic, V.; Walther, Ursula; Graner, Andreas

doi:10.1007/s001220051015

Cited by 55 publications

(32 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…spontaneum as a donor for resistance genes, novel alleles have been introgressed into the gene pool of cultivated barley. The typical outcome of such introgressions has been analyzed elsewhere (Ivandic et al 1998;Pillen et al 2004). While it initially generates a spike in genetic diversity, strong selection for the exotic allele will gradually erode the frequencies of the ''old'' alleles, even leading to their complete loss (Russell et al 2000;Collins et al 2001;Bundock and Henry 2004).…”

Section: Discussionmentioning

confidence: 99%

Effects of Introgression and Recombination on Haplotype Structure and Linkage Disequilibrium Surrounding a Locus EncodingBymovirusResistance in Barley

Stracke

Presterl²,

Stein

et al. 2007

Genetics

View full text Add to dashboard Cite

We present a detailed analysis of linkage disequilibrium (LD) in the physical and genetic context of the barley gene Hv-eIF4E, which confers resistance to the barley yellow mosaic virus (BYMV) complex. Eightythree SNPs distributed over 132 kb of Hv-eIF4E and six additional fragments genetically mapped to its flanking region were used to derive haplotypes from 131 accessions. Three haplogroups were recognized, discriminating between the alleles rym4 and rym5, which each encode for a spectrum of resistance to BYMV. With increasing map distance, haplotypes of susceptible genotypes displayed diverse patterns driven mainly by recombination, whereas haplotype diversity within the subgroups of resistant genotypes was limited. We conclude that the breakdown of LD within 1 cM of the resistance gene was generated mainly by susceptible genotypes. Despite the LD decay, a significant association between haplotype and resistance to BYMV was detected up to a distance of 5.5 cM from the resistance gene. The LD pattern and the haplotype structure of the target chromosomal region are the result of interplay between low recombination and recent breeding history.T HE ever-increasing availability of nucleotide sequence, and the concomitant improvement that this brings to our understanding of the organization of complex crop plant genomes, has created the opportunity to identify trait-related genes and to analyze their allelic diversity. The association between phenotype and genotype in diverse populations represents a powerful approach, but the applicability and design of such analyses depend critically on the extent and pattern of linkage disequilibrium (LD) present in the study population. Cultivated barley (Hordeum vulgare ssp. vulgare) has many of the hallmarks known to be associated with a high level of LD. Its effective recombination rate is dramatically reduced by its predominantly inbreeding habit, with an estimated outcrossing rate of 5% in winter barley and ,0.5% in spring barley (Giles et al. 1974;Doll 1987;Abdel-Ghani et al. 2005). Domestication and intensive selection have introduced major bottlenecks in genetic variation, and these are thought to be largely responsible for the perceived narrowness of the modern gene pool (Badr et al. 2000;Russell et al. 2000;Matus and Hayes 2002). LD in modern spring barley, as estimated from a whole-genome survey, extends over distances of at least 10 cM, indicative of an extensive conservation of the genetic identity of barley chromosomes (Kraakman et al. 2004). However, estimates of genomewide LD conceal localized variation, which, as has been shown for a number of species, can be substantial and independent of the mating system (Gupta et al. 2005). In the self-pollinating species Arabidopsis thaliana LD varies from ,10 kb in global (Tian et al. 2002) to 50-250 kb in local populations (Nordborg et al. , 2005Aranzana et al. 2005). Within a given set of maize (an outbreeding species) accessions, the extent of LD has been documented to vary widely around different genes and chr...

show abstract

Section: Discussionmentioning

confidence: 99%

Effects of Introgression and Recombination on Haplotype Structure and Linkage Disequilibrium Surrounding a Locus EncodingBymovirusResistance in Barley

Stracke

Presterl²,

Stein

et al. 2007

Genetics

View full text Add to dashboard Cite

show abstract

“…These lines were used to determine the chromosomal location of molecular markers linked to the resistance gene. Preliminary mapping information suggested that Rph15 may reside near Rph16 (Ivandic et al 1998) on chromosome 2HS. To resolve the allelic relationship between the resistance genes, crosses were made between Bowman (Rph15) and HS084, the source of Rph16.…”

Section: Plant Materialsmentioning

confidence: 98%

“…spontaneum) collections have been evaluated by several research groups in an effort to identify leaf rust resistance with potentially greater durability (Manisterski et al 1986;Feuerstein et al 1990;Moseman et al 1990;Jin et al 1995). Several new resistance genes were identified from this research and designated Rph10, Rph11, Rph15 and Rph16 (Feuerstein et al 1990;Chicaiza 1996;Franckowiak et al 1997;Ivandic et al 1998). Unfortunately, leaf rust pathotypes with virulence for some of these recently identified Rph genes (e.g., Rph10 and Rph11) are fairly common (Fetch et al 1998;B.…”

Section: Introductionmentioning

confidence: 93%

“…In addition to the above markers, the previously mapped AFLP marker E4140-8 (Castiglioni et al 1998) was found to be linked at 2.6 cM proximal to Rph15. An additional marker was obtained from MWG2133, an RFLP marker that was amplified and sequenced based on primers from the MWG2133 sequence deposited in the NCBI database (Ivandic et al 1998; accession numbers AJ234791 and AJ234792). The MWG2133 sequence from Bowman (Rph15), deposited in the NCBI database under accession number AY217120, contains an indel of 25 bp, making it possible to use this as a sequence tagged site (STS) Ivandic et al (1998).…”

Section: Mapping Of Rph15 In the Bowman (Rph15)bowman Populationmentioning

confidence: 99%

“…The development of various molecular markers for barley has greatly facilitated the precise mapping of leaf rust resistance genes. Using molecular markers, Rph2 was mapped to chromosome 5H (Borovkova et al 1997), Rph5, Rph6 (now designated Rph5.f) and Rph7 to the telomeric region of chromosome 3H (Brunner et al 2000;Graner et al 2000;Mammadov et al 2003;Zhong et al 2003), Rph9.i and Rph9.z to chromosome 5H (Borovkova et al 1998), Rph16 to chromosome 2H (Ivandic et al 1998) and Rph19 to chromosome 7H (Park and Karakousis 2002).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Conversion of an amplified fragment length polymorphism marker into a co-dominant marker in the mapping of the Rph15 gene conferring resistance to barley leaf rust, Puccinia hordei Otth.

2003

View full text Add to dashboard Cite

Leaf rust, caused by Puccinia hordei, is an important disease afflicting barley ( Hordeum vulgare) in many production regions of the world. The leaf rust resistance gene Rph15 was identified in an accession of wild barley ( Hordeum vulgare subsp. spontaneum) and is one of the most broadly effective resistance genes known. Using amplified fragment length polymorphism (AFLP) and simple sequence repeat markers, Rph15 was mapped to chromosome 2HS in an F(2) population derived from a cross between Bowman ( Rph15), a Bowman backcross-derived line carrying Rph15, and the susceptible cultivar Bowman. AFLP marker P13M40 co-segregated with Rph15 in this mapping population and two others involving Bowman ( Rph15) and cultivars Proctor and Nudinka. The dominant AFLP marker P13M40 was converted to a co-dominant PCR-based marker that may be useful in breeding programs employing marker-assisted selection. The allelic relationship between Rph15 and the gene Rph16, also mapping to chromosome 2HS, was studied. The lack of segregation in F(2) progeny derived from the two resistance sources indicates that Rph15 and Rph16 are alleles of the same locus.

show abstract

Development of barley introgression lines carrying the leaf rust resistance genes Rph1 to Rph15

et al. 2020

View full text Add to dashboard Cite

In many production areas, barley (Hordeum vulgare L.) is attacked by the leaf rust pathogen (Puccinia hordei Otth), a basidiomycetous fungus that reduces both its yield and quality. Many leaf rust resistance genes, known as reaction to P. hordei (Rph) genes, have been described in barley. To differentiate genetic variants for virulence in pathogen populations, plant pathologists use differentials (i.e., sets of host lines carrying different resistance genes). The sources of Rph1–15 were derived from cultivars, landraces, and wild barley (H. vulgare ssp. spontaneum K. Koch) accessions with diverse geographic origins and agromorphological traits. Ideal differential sets comprise single‐gene lines backcrossed to a single adapted accession that is susceptible to all known races of a pathogen. In this study, sources of Rph1–15 and other Rph gene donors were backcrossed to the susceptible barley cultivar Bowman and then genotyped to characterize the chromosomal positions and sizes of introgressions. Overall, 95 Bowman introgression lines for leaf rust resistance were developed and characterized for their rust phenotypes and genotypes. A single line was selected to represent each of the 15 Rph genes for use as the new barley leaf rust differential set. The existence of possible new resistance genes in the studied germplasm was postulated. The new Bowman Rph1–15 differential lines will facilitate the efficient virulence phenotyping of P. hordei and serve as valuable genetic stocks for Rph gene stacking and cloning in barley.

show abstract

Molecular mapping of a new gene in wild barley conferring complete resistance to leaf rust (Puccinia hordei Otth)

Cited by 55 publications

References 22 publications

Effects of Introgression and Recombination on Haplotype Structure and Linkage Disequilibrium Surrounding a Locus EncodingBymovirusResistance in Barley

Effects of Introgression and Recombination on Haplotype Structure and Linkage Disequilibrium Surrounding a Locus EncodingBymovirusResistance in Barley

Conversion of an amplified fragment length polymorphism marker into a co-dominant marker in the mapping of the Rph15 gene conferring resistance to barley leaf rust, Puccinia hordei Otth.

Development of barley introgression lines carrying the leaf rust resistance genes Rph1 to Rph15

Contact Info

Product

Resources

About