Molecular mapping of adult plant stripe rust resistance in wheat and identification of pyramided QTL genotypes

Abstract

The Puccinia striiformis f. sp. tritici (Pst) pathotype, 134 E16A?, detected in 2002 in Australia, produced relatively lower and higher adult plant stripe rust responses, respectively, on cultivars Kukri and Janz in comparison to the pre-2002 Pst pathotype 110 E143A?. Molecular mapping of adult plant stripe rust response variation among 180 Kukri/Janz-derived doubled haploid lines over 4 years, two each with Pst pathotypes 110 E143A? and 134 E16A?, was performed. QYr.sun-7B and QYr.sun-7D were consistently contributed by Kukri and Janz, respectively. QYr.sun-7D corresponded to the genomic location of Yr18 and QYr.sun-7B remains to be formally named. QYr.sun-1B, QYr.sun-5B, and QYr.sun-6B were detected during more than one season irrespective of the Pst pathotypes used, whereas QYr.sun-3B was identified only during the 2003 crop season. QYr.sun-1A contributed by Janz, and QYr.sun-2A from Kukri, were detected only against Pst pathotypes 110 E143A? and 134 E16A?, respectively. The DH lines showing better resistance than the either parent carried combinations of 4 to 6 QTL. These lines are currently being used as stripe rust resistance donors in wheat breeding programs.

Identification of adult plant resistance to stripe rust in the wheat cultivar Cappelle-Desprez

“…In the study of Boukhatem et al (2002), QYR2 accounted for only 15.4% of the phenotypic variation. Bariana et al (2010) identified an APR QTL proximal to the region defining QYr. Hao et al 2011).…”

“…Oligoculm (Suenaga et al 2003), the Australian cv. Janz (Bariana et al 2010) and the French cv. Flinor (Feng et al 2011).…”

Quantitative trait loci of stripe rust resistance in wheat

“…This had been identified in numerous studies and is described as group 3 on 1BL (QRYr1B.3) in Table 1 (Bariana et al 2001, 2010; Jagger et al 2011; Lillemo et al 2008; Melichar et al 2008; Rosewarne et al 2008, 2012; William et al 2006; Zwart et al 2010). There is a wide range in the LOD (2.8–23) and PEV (4.5–65) scores indicating the variability that this locus can contribute.…”

“…However, Yr29 is important as is demonstrated by the number of significant environments tested. Nearly every study finds this locus significant in every environment tested with the exception of Bariana et al (2010). Interestingly, Bariana et al (2010) also had Lr34/Yr18 (hereafter termed Yr18 ) segregating in that population and it is now becoming apparent that when those two loci are in the same genetic background, the Yr29 locus has lesser effect (Lillemo et al 2008; Suenaga et al 2003; Yang et al 2013).…”

“…1. Chromosome 1A had four QTLs described (Bariana et al 2010; Dedryver et al 2009; Ren et al 2012a; Rosewarne et al 2012). The flanking markers of these form two distinct groups although they are both on the long arm of chromosome 1A.…”