Molecular mapping of bunchy top disease resistance in Gossypium hirsutum L.

Abstract

Cotton bunchy top (CBT) is an aphid transmitted Polerovirus disease and a significant threat to the Australian cotton industry. Symptoms include stunted plant growth, and leaves often display pale green angular patterns at the leaf margins and dark green centers with a leathery texture. Resistance to CBT was evaluated in 206 F 2 plants and 76 F 2:3 families derived from the resistant cultivar 'Delta Opal' crossed to the susceptible cultivar 'Sicot 70', and in 25 other cultivars the majority susceptible to CBT. CBT resistance in 'Delta Opal' was shown to be controlled by a single dominant locus designated Cbt. A combination of AFLP and single nucleotide polymorphism markers located Cbt on chromosome A10, close to the mapped resistance locus in 'Delta Opal' to another Polerovirus disease of cotton; cotton blue disease. The markers identified flanking CBT resistance will provide useful tools for breeders for markerassisted selection to alleviate the impact of this disease on cotton production.

Diversity analysis of cotton (Gossypium hirsutum L.) germplasm using the CottonSNP63K Array

“…A separate study targeting SNPs for genetic male sterility ( ms ) in cotton [ 70 ] successfully linked a SNP to the recessive gene ms5 on chromosome 12 (A12) and ms6 on chromosome 26 (D12). Similarly, Ellis et al [ 71 ] used the array to identify markers linked to a viral resistance gene for cotton bunchy top disease. This targeted SNP association analysis identified nearly as good an interval spanning the Cbd resistance locus as was obtained from screening an F 2:3 population.…”

P0 protein of cotton leafroll dwarf virus‐atypical isolate is a weak RNA silencing suppressor and the avirulence determinant that breaks the cotton Cbd gene‐based resistance

“…Moreover, CBD‐resistant cotton varieties are also resistant to cotton bunchy top virus (CBTV), a recombinant virus between the 5′ region of CLRDV and the 3′ of an uncharacterized polerovirus. The CBTV resistance gene was also mapped to the same telomeric region where 11 genes with NB‐LRR domains were identified (Ellis et al ., ).…”

Genetic dissection of the fuzzless seed trait in Gossypium barbadense

“…In cotton, a large number of single nucleotide polymorphism (SNP) markers have been reported based on whole genome re-sequencing of diverse cotton cultivars and transcriptome sequencing ( Byers et al , 2012 ; Zhu et al , 2014 b ; Hulse-Kemp et al , 2015 a ; Wang et al , 2015 ; Fang et al , 2017 ). A cotton SNP array containing ~63 000 SNPs, mainly from US and Australian cotton cultivars, was recently developed ( Hulse-Kemp et al , 2015 b ) and has been widely used by the cotton community for a diversity of studies ( Ellis et al , 2016 ; Li et al , 2016 ; Wang et al , 2016 ; Gapare et al , 2017 ; Hinze et al , 2017 ; Huang et al , 2017 a ), including identification of the gene responsible for okra leaf shape ( Zhu et al , 2016 ). One of the applications of next-generation sequencing in identification of genes underlying specific phenotypes is mapping-by-sequencing (MBS) where pools of segregating progeny are bulked according to their mutant phenotype and their genome sequenced to identify genomic regions inherited predominantly from the parent displaying the phenotype ( Zhu et al , 2017 ).…”