Molecular mapping of class II polymorphisms in the human major histocompatibility complex. I. DR beta.

Bell, J I; Denney, Dan W.; MacMurray, Armand J.; Foster, Luke; Watling, Diane; McDevitt, H O

doi:10.4049/jimmunol.139.2.562

The Journal of Immunology

1987

DOI: 10.4049/jimmunol.139.2.562

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Molecular mapping of class II polymorphisms in the human major histocompatibility complex. I. DR beta.

J I Bell¹,

Dan W. Denney²,

Armand J. MacMurray³

et al.

Abstract: We have studied 27 cell lines homozygous by consanguinity for the major histocompatibility complex to establish the restriction fragment length polymorphism (RFLP) patterns seen with six different restriction enzymes (Bam HI, Bg1 II, Eco RI, Hinc II, Hind III, Pvu II) and DR beta chain probes. The probes used were a full-length cDNA DR beta probe and a probe specific for the 3' untranslated region. The RFLP obtained represent the first standard patterns for the individual haplotypes DR1 through 7 and DR9 as de… Show more

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HLA-D Locus Associations in Alopecia Areata

et al. 1991

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Because predisposition to autoimmunity has been associated with HLA-D alleles and alopecia areata is hypothesized to be a T-cell mediated autoimmune hair loss, we determined DR and DQ alleles in 88 white and 10 American black patients with alopecia areata as well as controls with the use of restriction fragment length polymorphism typing with cDNA probes. White patients with alopecia areata have an increase in the phenotype frequencies of DR4 and DQw8 and an increase in genotype frequencies of DR4 and DR5 (now DRw11[5]). These associations are in agreement with those reported in two other studies but are not significant when corrected by the number of HLA antigens tested. Sixty-one percent of all patients with AA have DR4 and/or DRw11(5) specificities vs 40% of controls, with more DR4,DRw11(5) and DQw7(w3), DQw8(w3) heterozygotes among patients. DQw6(w1) phenotype frequencies and DRw52a phenotype and genotype frequencies are significantly decreased in patients with alopecia areata relative to controls. This highly significant negative association with the HLA DRB3 allele DRw52a in whites persisted even when DR4- or DRw11(5)-positive individuals were excluded from the patient and control groups. These data suggest that HLA-DR4 and DRw11(5) with their associated DQw7(w3) and DQw8(w3) specificities may confer susceptibility to alopecia areata, while DRw52a may confer resistance.

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HLA-D Locus Associations in Alopecia Areata

et al. 1991

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DNA analysis of HLA–DR and DQ genes in american blacks with systemic lupus erythematosus

Reveille

Schrohenloher

Acton

et al. 1989

Arthritis & Rheumatism

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We studied DNA polymorphisms of HLA-DR and DQ alleles in 63 American black patients with systemic lupus erythematosus (SLE). We found no HLA-DR beta, DQ alpha, or DQ beta restriction fragment length polymorphism (RFLP) or RFLP-determined DR or DQ specificity associated with SLE in either the patients or in 57 control subjects. DRw52b was positively associated with renal involvement and negatively associated with anti-nuclear RNP antibodies. Antibodies to Ro (SS-A) and La (SS-B) were associated with DR3(DRw17), DQw2.3. Early-onset SLE (less than or equal to 20 years of age) was associated with DRw8, and the frequency of neuropsychiatric involvement correlated negatively with a 3.7-kb Taq I DQ alpha RFLP. This suggests a role for DR and DQ genes in the clinical and serologic expression of SLE in American blacks.

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HLA‐D Region genes associated with autoantibody responses to histidyl‐transfer RNA synthetase (Jo‐1) and other translation‐related factors in myositis

Goldstein

Duvic

Targoff

et al. 1990

Arthritis & Rheumatism

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Myositis has been associated with HLA-B8 and DR3, especially in white patients with polymyositis and serum anti-Jo-1 antibodies. Twenty-eight patients with myositis and serum translation-related autoantibodies anti-Jo-1, anti-PL-7, anti-PL-12, anti-KJ, and anti-SRP were studied for HLA class I1 specificities by Southern blotting with HLA-DRP, DQP, and D Q a probes. The association of HLA-DR3 (DRwl7) with anti-Jo-1 antibodies in white myositis patients was confirmed (P = 0.003, relative risk 8.9). However, HLA-DRw52 haplotypes, regardless of subtype, were present in all of the white and black patients with serum anti-Jo-1 and other translation-related autoantibodies. Moreover, one anti-Jo-1 positive patient had HLADRw8, an HLA-DRw52 haplotype on which the DRp3 gene has been partially deleted. No HLA-DQ specificity or allele was common to all patients. The HLA-DR3, DRS, DRw6, and DRw8 haplotypes, which bear the HLA-DRw52 specificity, share the most homology in the DRPl first hypervariable region at amino acid positions 9-13. Thus, this DRPl region appears to be the most likely candidate "epitope" for translation-related autoimmune responses in inflammatory myositis.Inflammatory myositis, particularly polymyositis in adults and dermatomyositis in children, has been associated with HLA-B8 and DR3 in white individuals (1,2). In an earlier study using serologic HLA typing (3), the specific subset of myositis patients with serum anti-histidyl-transfer RNA (tRNA) synthetase (antiJo-1) autoantibodies (4) had an even higher frequency of HLA-DR3 than myositis patients overall; all 11 patients with serum anti-Jo-1 antibodies had either HLA-DR3 and/or HLA-DRw6. It was hypothesized that this autoimmune response in myositis may be

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Molecular mapping of class II polymorphisms in the human major histocompatibility complex. I. DR beta.

Cited by 50 publications

References 0 publications

HLA-D Locus Associations in Alopecia Areata

HLA-D Locus Associations in Alopecia Areata

DNA analysis of HLA–DR and DQ genes in american blacks with systemic lupus erythematosus

HLA‐D Region genes associated with autoantibody responses to histidyl‐transfer RNA synthetase (Jo‐1) and other translation‐related factors in myositis

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