Molecular mapping of five soybean genes involved in male-sterility, female-sterility

Abstract

In soybean, asynaptic and desynaptic mutants lead to abnormal meiosis and fertility reduction. Several male-sterile, female-sterile mutants have been identified and studied in soybean, however, some of these mutants have not been mapped to locations on soybean chromosomes. The objectives of this study were to molecularly map five male-sterile, female-sterile genes (st2, st4, st5, st6, and st7) in soybean and compare the map locations of these genes with already mapped sterility genes. Microsatellite markers were used in bulked segregant analyses to locate all five male-sterile, female-sterile genes to soybean chromosomes, and markers from the corresponding chromosomes were used on F2 populations to generate genetic linkage maps. The st2, st4, st5, st6, and st7 genes were located on molecular linkage group (MLG) B1 (chromosome 11), MLG D1a (chromosome 01), MLG F (chromosome 13), MLG B2 (chromosome 14), and D1b (chromosome 02), respectively. The st2, st4, st5, st6, and st7 genes were flanked to 10.3 (∼ 399 kb), 6.3 (∼ 164 kb), 3.9 (∼ 11.8 Mb), 11.0 (∼ 409 kb), and 5.3 cM (∼ 224 kb), and the flanked regions contained 57, 17, 362, 52, and 17 predicted genes, respectively. Future characterization of candidate genes should facilitate identification of the male- and female-fertility genes, which may provide vital insights on structure and function of genes involved in the reproductive pathway in soybean.

Male sterility in soybean: Occurrence, molecular basis and utilization

“…Up to date, more than 30 soybean NMS systems have been identified which include the following: structural MS ( fs1fs2 ), partially MS ( msp ), male and female sterility ( St1 ), MS and female fertile ( ms1‐ms9 ), photosensitive MS ( 88‐428‐BY ) and MS controlled by a single‐dominant gene ( N7241S ). However, there is no evidence on the molecular cloning of a MS gene in soybean (Speth et al, ). Due to the numerous varieties of the restorer line and scarce maintainer line of NMS, it is quite difficult to achieve the production of three‐system breeding, which limits the practical application of NMS.…”

“…The MFMF gene from male‐sterile and female‐sterile soybean mutant was mapped to LG “D1a”, close to the marker, Satt531 with 9.4 cM distance from the gene (Baumbach et al, ). Speth et al () found that five NMS‐related genes ( st2 , st4 , st5 , st6 and st7 ) in soybean were located on LG “B1”, LG “D1a”, LG “F”, LG “B2” and LG “D1b”, respectively. The st2 , st4 , st5 , st6 and st7 genes were flanked to 10.3 cM (~399 kb), 6.3 cM (~164 kb), 3.9 cM (~11.8 Mb), 11.0 cM (~409 kb) and 5.3 cM (~224 kb), and that flanked regions comprised 57, 17, 362, 52 and 17 predicted genes, respectively.…”

“…Moreover, over the past few decades, extensive researches on the different aspects of soybean MS (CMS and NMS system) had been conducted based on traditional breeding and development of modern molecular technology, which mainly included hybrid seed production and population improvement (Ding, Gai, Cui, & Qiu, ; Gai & Fehr, ; Grayboach & Palmer, ; Jin, Horner, & Palmer, ; Lewers & Palmer, ; Sun, Zhao, & Huang, ; Sun, Zhao, Wang, Wang, & Li, ; Wang et al, ; Xie, ; Zhao et al, , ), three‐line breeding (Bai & Gai, ; Dai et al, ; Ding et al, ; Li, Ding, et al, ; Nie, Zhao, Yang, & Gai, ; Zhao & Gai, ) and recurrent selection (Lewers & Palmer, ; Si, Wang, Qiu, Gai, & Burton, ; Song, Wu, & Gai, ; Zhao et al, ). In addition, cytology and structural characteristics (Bione, Pagliarini, & Almeida, ; Chen, Albertsen, & Palmer, ; Chen & Palmer, ; Ding & Gai, ; Zhang, Sun, et al, ), genetics (Bai & Gai, ; Baumbach et al, ; Cervantes‐Martinez et al, , ; Kato & Palmer, , ; Ott et al, ; Perez‐Sackett & Palmer, ; Speth et al, ; Yang et al, ), tissue structure (chloroplasts and mitochondria) (Bione, Pagliarini, & Almeida, ; Chang et al, ; Horn et al, ; Lin et al, ), epigenetics and omics level (Ding et al, ; Ding, Wang, et al, ; Ding, Zhang, et al, ; Han et al, ; Li et al, , ; Li, Yang, & Gai, ; Li, Ding, et al, ), localization and function of fertility and restoring gene traits were also studied (Baumbach et al, ; Dong et al, ; Huang et al, ; Jiang, Yang, Yu, & Gai, ; Jiang, Yang, Yu, & Gai, ; Raval et al, …”

Identification and Mapping of a New Soybean Male-Sterile Gene, mst-M

“…Sterility is a common phenomenon among plants. On the basis of the mode of inheritance, two main types of sterility have been identified in plants: cytoplasmic sterility and nucleus-dependent sterility (Zhang et al, 2008; Chen and Liu, 2014; Yang et al, 2014; Speth et al, 2015; Bohra et al, 2016; Chang et al, 2016; Liu et al, 2018; Xie et al, 2018). In plant breeding, hybrid seeds/lines are advantageous because they produce high yields.…”

“…Yang et al (2010) mapped two independent Rf loci linked to Satt626 in molecular linkage group (MLG) M and Satt300 in MLG A1 at genetic distances of 9.75 and 11.18 cM, respectively. With regard to GMS, more than 20 male-sterility loci have been reported, including ms1 – ms9 , msMOS , msp , st1 – st8 , ASR-7-206 , A03-2137 , A05-133 , A06-204 , and st_A06-2/6 (Owen, 1928; Hadley and Starnes, 1964; Palmer, 1974, 2000; Palmer et al, 1978, 2008; Delannay and Palmer, 1982; Palmer and Kaul, 1983; Graybosch and Palmer, 1985, 1988; Graybosch et al, 1987; Horner and Palmer, 1995; Jin et al, 1997, 1998; Palmer and Horner, 2000; Cervantes-Martinez et al, 2007, 2009; Rebeccaa et al, 2011; Baumbach et al, 2012), the majority of which have been mapped to a linkage group (Yang et al, 2014; Speth et al, 2015). However, none of these loci have been successfully cloned or fine-mapped.…”